CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Atkins, K. B.
Right arrow Articles by Troen, B. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atkins, K. B.
Right arrow Articles by Troen, B. R.

Cell Growth & Differentiation, Vol 6, Issue 6 713-718, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Phorbol ester stimulated cathepsin L expression in U937 cells

KB Atkins and BR Troen
Department of Internal Medicine, Veterans Administration Medical Center, University of Michigan, Ann Arbor 48109, USA.

Cathepsin L (ctsl) is a lysosomal cysteine proteinase, the synthesis and secretion of which is induced by transformation, growth factors, and tumor promoters. We studied the effect and the mechanism of action of phorbol ester (TPA) on the expression of ctsl mRNA in U937 histiocytic leukemia cells. TPA treatment induces ctsl mRNA in a manner that is dose-dependent, occurs at the level of transcription, and is ablated by cotreatment with cycloheximide but is unaffected by dexamethasone. Treatment with TPA plus staurosporine, a potent protein kinase C inhibitor, results in greater expression of ctsl mRNA than does treatment with TPA alone. Similar to TPA, staurosporine alone increases ctsl transcription, an effect that is inhibited by cycloheximide. Another PKC inhibitor, H7, exerted no effect upon the induction of ctsl mRNA by either TPA or staurosporine. Staurosporine and H7, however, inhibit the increase in c-jun mRNA by TPA. In contrast, the tyrosine kinase inhibitors herbimycin A and genistein inhibit the effect of TPA and staurosporine upon ctsl mRNA with little or no effect on c-jun expression. Pretreatment with sodium orthovanadate enhances the induction of ctsl expression by TPA and staurosporine. These data suggest that, in U937 cells, TPA-stimulated ctsl gene transcription is apparently activated by a protein kinase C-independent signal transduction pathway involving tyrosine kinase activation.


This article has been cited by other articles:


Home page
EMBO J.Home page
G. Amuthan, G. Biswas, S.-Y. Zhang, A. Klein-Szanto, C. Vijayasarathy, and N. G. Avadhani
Mitochondria-to-nucleus stress signaling induces phenotypic changes, tumor progression and cell invasion
EMBO J., April 17, 2001; 20(8): 1910 - 1920.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
Q. Li, S. M. Vaingankar, H. M. Green, and M. Martins-Green
Activation of the 9E3/cCAF Chemokine by Phorbol Esters Occurs via Multiple Signal Transduction Pathways That Converge to MEK1/ERK2 and Activate the Elk1 Transcription Factor
J. Biol. Chem., May 28, 1999; 274(22): 15454 - 15465.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Nagaya, Y. Murata, S. Yamaguchi, Y. Nomura, S. Ohmori, M. Fujieda, N. Katunuma, P. M. Yen, W. W. Chin, and H. Seo
Intracellular Proteolytic Cleavage of 9-cis-Retinoic Acid Receptor {alpha} by Cathepsin L-type Protease Is a Potential Mechanism for Modulating Thyroid Hormone Action
J. Biol. Chem., December 11, 1998; 273(50): 33166 - 33173.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
S. Ries, C. Buchler, T. Langmann, P. Fehringer, C. Aslanidis, and G. Schmitz
Transcriptional regulation of lysosomal acid lipase in differentiating monocytes is mediated by transcription factors Sp1 and AP-2
J. Lipid Res., November 1, 1998; 39(11): 2125 - 2134.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.