CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rosenberg, M. P.
Right arrow Articles by Propst, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rosenberg, M. P.
Right arrow Articles by Propst, F.

Cell Growth & Differentiation, Vol 6, Issue 3 325-336, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Expression of the v-Mos oncogene in male meiotic germ cells of transgenic mice results in metaphase arrest

MP Rosenberg, CR Aversa, R Wallace and F Propst
Glaxo Research Institute, Department of Pharmacology, Research Triangle Park, North Carolina 27709, USA.

To explore the role of pp39mos in male germ cell meiosis, we have constructed transgenic mice carrying either the c-Mos or v-Mos genes linked to the human male germ cell-specific phosphoglycerate kinase-2 promoter. All male transgenic mice bearing the v-Mos but not the c-Mos construct were sterile due to arrest of germ cells at metaphase I. Immunocytochemistry performed on sections from control and c-Mos transgenic testes with eight different monoclonal and polyclonal antisera against either alpha-, beta- or gamma-tubulins demonstrated that all could recognize MI spermatocyte spindles from control and c-Mos transgenics, but only one monoclonal anti-microtubule sera decorated the spindles of v-Mos-arrested meiotic figures. Western blot analyses with this one serum revealed a change in proteins in the v-Mos samples. Immunocytochemistry with the MPM-2 monoclonal antibody, which is specific for epitopes phosphorylated during mitosis, demonstrated an increase in cytoplasmic and spindle-associated phosphoproteins in arrested v-Mos spermatocytes. Western analysis with MPM-2 showed an increase in a M(r) 50,000-55,000 and a M(r) 25,000-29,000 protein in Mos transgenic testes when compared to controls. An anti-MAP kinase antibody demonstrated an increase in all four MAP kinases in testes of transgenic mice. Thus, overexpression of pp39v-mos during male germ cell meiosis resulted in an alteration of various cell cycle related kinases and cytostatic factor-like arrest at MI.


This article has been cited by other articles:


Home page
ReproductionHome page
S. Kimmins, N. Kotaja, I. Davidson, and P. Sassone-Corsi
Testis-specific transcription mechanisms promoting male germ-cell differentiation
Reproduction, July 1, 2004; 128(1): 5 - 12.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
Y. T. Kwon, Z. Xia, J. Y. An, T. Tasaki, I. V. Davydov, J. W. Seo, J. Sheng, Y. Xie, and A. Varshavsky
Female Lethality and Apoptosis of Spermatocytes in Mice Lacking the UBR2 Ubiquitin Ligase of the N-End Rule Pathway
Mol. Cell. Biol., November 15, 2003; 23(22): 8255 - 8271.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
X. Meng, H. Akutsu, K. Schoene, C. Reifsteck, E. P. Fox, S. Olson, H. Sariola, R. Yanagimachi, and M. Baetscher
Transgene Insertion Induced Dominant Male Sterility and Rescue of Male Fertility Using Round Spermatid Injection
Biol Reprod, March 1, 2002; 66(3): 726 - 734.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.