CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quincoces, A. F.
Right arrow Articles by Leon, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quincoces, A. F.
Right arrow Articles by Leon, J.

Cell Growth & Differentiation, Vol 6, Issue 3 271-279, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Serum growth factors up-regulate H-ras, K-ras, and N-ras proto-oncogenes in fibroblasts

AF Quincoces and J Leon
Departamento de Biologia Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.

We have analyzed the expression of the ras gene family during the transit through quiescence-proliferation. H-, K- and N-ras steady-state mRNA levels and total Ras protein levels did not change significantly when NIH3T3 cells were made quiescent by density arrest in the presence of 10% calf serum. By contrast, levels of ras mRNAs in cells that had been made quiescent by serum deprivation were lower than those in growing cells. An induction of H-, K- and N-ras mRNA levels (3- to 5-fold) was detected in these cells after serum addition. This induction was maximal around 8 h after serum addition for the three ras genes. Like the early-response genes, ras induction was not dependent on protein synthesis; but in contrast to these genes, ras mRNAs showed long half-lives (5-7 h) in NIH3T3 cells. Up-regulation of ras genes by serum was also observed in human primary fibroblasts, indicating that this may be a general effect in mammal cells. We obtained stable transfectants in NIH3T3 cells with the oncogenic N-ras. In these cells, expression of the transforming gene is also induced by serum, and the expression of the transfected N-ras gene did not modify the response to serum of endogenous H-, K-, and N-ras genes. The regions of murine N-ras gene responsible for serum inducibility seem to be intragenic because N-ras up-regulation occurred in cells transfected with a gene construct lacking the sequences upstream from the first exon.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
Mol Cancer ResHome page
J. P. Vaque, B. Fernandez-Garcia, P. Garcia-Sanz, N. Ferrandiz, G. Bretones, F. Calvo, P. Crespo, M. C. Marin, and J. Leon
c-Myc Inhibits Ras-Mediated Differentiation of Pheochromocytoma Cells by Blocking c-Jun Up-Regulation
Mol. Cancer Res., February 1, 2008; 6(2): 325 - 339.
[Abstract] [Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
A. M. Taylor and C. A. McNamara
Regulation of Vascular Smooth Muscle Cell Growth: Targeting the Final Common Pathway
Arterioscler Thromb Vasc Biol, October 1, 2003; 23(10): 1717 - 1720.
[Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.