CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marthinuss, J.
Right arrow Articles by Seiberg, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marthinuss, J.
Right arrow Articles by Seiberg, M.

Cell Growth & Differentiation, Vol 6, Issue 3 239-250, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Apoptosis in Pam212, an epidermal keratinocyte cell line: a possible role for bcl-2 in epidermal differentiation

J Marthinuss, L Lawrence and M Seiberg
Skin Biology Research Center, R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 08869, USA.

Programmed cell death is a controlled process that leads to the elimination of single cells via apoptosis, a mode of cell death with a characteristic morphology. During epidermal differentiation, keratinocytes migrate outward to become terminally differentiated cornified cells in a process involving programmed cell death pathway(s) and apoptosis. The molecular mechanisms regulating epidermal differentiation and apoptosis have not yet been elucidated. Here we show that a mouse keratinocyte cell line, Pam212, undergoes spontaneous apoptosis in culture. Apoptosis of Pam212 cells is demonstrated by both morphology and DNA oligonucleosomal degradation. The expression of bcl-2, a gene implicated in the negative control of apoptosis, was down-regulated in these cells by transfecting a bcl-2-antisense expression vector. The cells that down-regulate bcl-2 expression exhibit enhanced apoptosis and further progress in the epidermal differentiation pathway. We analyzed the expression patterns of several genes that have been implicated in apoptosis in other systems. We show that the mRNA levels of c-myc, c-myb, c-fos, tumor necrosis factors (TNF) alpha and beta, TNF receptors I and II, interleukin 1 alpha, IFN-gamma, and transforming growth factor beta increase in the antisense-transfected cells. We suggest that bcl-2 influences epidermal differentiation in Pam212 keratinocyte cells, and maybe in vivo, by negatively regulating several genes that are involved in apoptosis.


This article has been cited by other articles:


Home page
J. Virol.Home page
J. Webster-Cyriaque, J. Middeldorp, and N. Raab-Traub
Hairy Leukoplakia: an Unusual Combination of Transforming and Permissive Epstein-Barr Virus Infections
J. Virol., August 15, 2000; 74(16): 7610 - 7618.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
C. W. Dawson, J. Dawson, R. Jones, K. Ward, and L. S. Young
Functional Differences between BHRF1, the Epstein-Barr Virus-Encoded Bcl-2 Homologue, and Bcl-2 in Human Epithelial Cells
J. Virol., November 1, 1998; 72(11): 9016 - 9024.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
A. Kocikova, A. Kolesaric, F. Koszik, G. Stingl, and A. Elbe-Burger
Murine Langerhans Cells Cultured Under Serum-Free Conditions Mature into Potent Stimulators of Primary Immune Responses In Vitro and In Vivo
J. Immunol., October 15, 1998; 161(8): 4033 - 4041.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
P. Mehlen, A. Mehlen, J. Godet, and A.-P. Arrigo
hsp27 as a Switch between Differentiation and Apoptosis in Murine Embryonic Stem Cells
J. Biol. Chem., December 12, 1997; 272(50): 31657 - 31665.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. D. Hines and B. L. Allen-Hoffmann
Keratinocyte Growth Factor Inhibits Cross-linked Envelope Formation and Nucleosomal Fragmentation in Cultured Human Keratinocytes
J. Biol. Chem., March 15, 1996; 271(11): 6245 - 6251.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.