Cell Growth & Differentiation, Vol 6, Issue 2 199-210, Copyright © 1995 by American Association of Cancer Research

ARTICLES

Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner

PL Chen, YC Ueng, T Durfee, KC Chen, T Yang-Feng and WH Lee
Center for Molecular Medicine/Institute of Biotechnology, University of Texas Health Science Center at San Antonio 78245, USA.

The full-length cDNA clone which encodes a novel 824-amino acid protein was characterized. The predicted protein contains ten 34-amino acid repeats characteristic of the tetratricopeptide repeat protein family. The sequence homology and organization of the 10 repeats are similar to those of the nuc2 protein of fission yeast and bimA protein of Aspergillus, which suggests that the newly identified protein could be the human homologue of nuc2 (H-NUC). Consistent with this notion, the M(r) 95,000 H-NUC is a nuclear protein with DNA binding activity. This protein binds to hypophosphorylated Rb protein in a region indistinguishable from that to which SV40 large T antigen binds. However, Rb also binds to H-NUC at the tetratricopeptide repeat motif, a region which contains sequences different from the binding motifs of either T-antigen or E2F-1. To mimic the temperature-sensitive mutant of yeast nuc2, an H-NUC mutant was made in which the highly conserved glycine 640 residue was changed to aspartic acid. In contrast to wild-type H-NUC, the mutant was temperature sensitive in binding to Rb protein. These results, taken together, suggest that the interaction between H-NUC and Rb may be significant.

This article has been cited by other articles:

				G. J. Poggioli, R. L. DeBiasi, R. Bickel, R. Jotte, A. Spalding, G. L. Johnson, and K. L. Tyler Reovirus-Induced Alterations in Gene Expression Related to Cell Cycle Regulation J. Virol., February 22, 2002; 76(6): 2585 - 2594. [Abstract] [Full Text] [PDF]

				L. Zheng, Y. Chen, D. J. Riley, P.-L. Chen, and W.-H. Lee Retinoblastoma Protein Enhances the Fidelity of Chromosome Segregation Mediated by hsHec1p Mol. Cell. Biol., May 15, 2000; 20(10): 3529 - 3537. [Abstract] [Full Text]

				K. L. van Golen, S. Davies, Z. Fen Wu, Y. Wang, C. D. Bucana, H. Root, S. Chandrasekharappa, M. Strawderman, S. P. Ethier, and S. D. Merajver A Novel Putative Low-Affinity Insulin-like Growth Factor-binding Protein, LIBC (Lost in Inflammatory Breast Cancer), and RhoC GTPase Correlate with the Inflammatory Breast Cancer Phenotype Clin. Cancer Res., September 1, 1999; 5(9): 2511 - 2519. [Abstract] [Full Text] [PDF]

				L. Zheng, Y. Chen, and W.-H. Lee Hec1p, an Evolutionarily Conserved Coiled-Coil Protein, Modulates Chromosome Segregation through Interaction with SMC Proteins Mol. Cell. Biol., August 1, 1999; 19(8): 5417 - 5428. [Abstract] [Full Text] [PDF]

				R. Wang, X. Wang, A. C. Atwood, S. L. Topalian, and S. A. Rosenberg Cloning Genes Encoding MHC Class II-Restricted Antigens: Mutated CDC27 as a Tumor Antigen Science, May 21, 1999; 284(5418): 1351 - 1354. [Abstract] [Full Text]

				S. Li, C.-Y. Ku, A. A. Farmer, Y.-S. Cong, C.-F. Chen, and W.-H. Lee Identification of a Novel Cytoplasmic Protein That Specifically Binds to Nuclear Localization Signal Motifs J. Biol. Chem., March 13, 1998; 273(11): 6183 - 6189. [Abstract] [Full Text] [PDF]

				V. Ollendorff and D. J. Donoghue The Serine/Threonine Phosphatase PP5 Interacts with CDC16 and CDC27, Two Tetratricopeptide Repeat-containing Subunits of the Anaphase-promoting Complex J. Biol. Chem., December 19, 1997; 272(51): 32011 - 32018. [Abstract] [Full Text] [PDF]

				Y. Chen, Z. D. Sharp, and W.-H. Lee HEC Binds to the Seventh Regulatory Subunit of the 26 S Proteasome and Modulates the Proteolysis of Mitotic Cyclins J. Biol. Chem., September 19, 1997; 272(38): 24081 - 24087. [Abstract] [Full Text] [PDF]

				P L Chen, D J Riley, Y Chen, and W H Lee Retinoblastoma protein positively regulates terminal adipocyte differentiation through direct interaction with C/EBPs. Genes & Dev., November 1, 1996; 10(21): 2794 - 2804. [Abstract] [PDF]

				R L Smith, M J Redd, and A D Johnson The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2. Genes & Dev., December 1, 1995; 9(23): 2903 - 2910. [Abstract] [PDF]