CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denning, M. F.
Right arrow Articles by Yuspa, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denning, M. F.
Right arrow Articles by Yuspa, S. H.

Cell Growth & Differentiation, Vol 6, Issue 2 149-157, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Specific protein kinase C isozymes mediate the induction of keratinocyte differentiation markers by calcium

MF Denning, AA Dlugosz, EK Williams, Z Szallasi, PM Blumberg and SH Yuspa
Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892, USA.

The maturation of epidermal keratinocytes is a tightly regulated, stepwise process which requires protein kinase C (PKC) activation. We investigated the effect of elevated extracellular Ca2+, a potent differentiation signal which increases cellular sn-1,2-diacylglycerol levels, on the PKC isozyme profile of cultured murine keratinocytes. Five PKC isozymes (alpha, delta, epsilon, zeta, and eta) were detected by immunoblotting. During Ca(2+)-induced differentiation, total cellular PKC alpha decreased, PKC epsilon and eta increased 3-5-fold, and the level of other PKC isozymes was relatively unchanged. PKC alpha underwent a progressive translocation from the soluble to the particulate fraction following elevation of extracellular Ca2+. The kinetics of PKC alpha translocation corresponded with the induction of keratinocyte differentiation markers. Both PKC delta and epsilon were selectively lost from the soluble fraction of keratinocytes exposed to elevated extracellular Ca2+, resulting in an increase in the proportion of these isoforms in the particulate fraction. PKC eta increased in both the soluble and particulate fractions, while PKC zeta did not change in amount or distribution during keratinocyte differentiation. Selective down-regulation of PKC isoforms by either 12-deoxyphorbol-13-phenylacetate or bryostatin 1 inhibited Ca(2+)-induced expression of differentiation markers at doses most specific for the down-regulation of PKC alpha. Taken together, these observations suggest that the induction of keratinocyte differentiation by Ca2+ results in the activation of specific PKC isozymes.


This article has been cited by other articles:


Home page
J. Lipid Res.Home page
M. Chae, J.-Y. Jung, I.-H. Bae, H.-J. Kim, T. R. Lee, and D. W. Shin
Lipin-1 expression is critical for keratinocyte differentiation
J. Lipid Res., April 1, 2016; 57(4): 563 - 573.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
Y. C. Chew, G. Adhikary, W. Xu, G. M. Wilson, and R. L. Eckert
Protein Kinase C {delta} Increases Kruppel-like Factor 4 Protein, which Drives Involucrin Gene Transcription in Differentiating Keratinocytes
J. Biol. Chem., June 14, 2013; 288(24): 17759 - 17768.
[Abstract] [Full Text] [PDF]


Home page
Anticancer ResHome page
E. KRASNITSKY, Y. BAUMFELD, J. FREEDMAN, N. SION-VARDY, S. ARIAD, V. NOVACK, and E. LIVNEH
PKC{eta} Is a Novel Prognostic Marker in Non-small Cell Lung Cancer
Anticancer Res, April 1, 2012; 32(4): 1507 - 1513.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
H. Raveh-Amit, A. Maissel, J. Poller, L. Marom, O. Elroy-Stein, M. Shapira, and E. Livneh
Translational Control of Protein Kinase C{eta} by Two Upstream Open Reading Frames
Mol. Cell. Biol., November 15, 2009; 29(22): 6140 - 6148.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
S. Getsios, C. L. Simpson, S.-i. Kojima, R. Harmon, L. J. Sheu, R. L. Dusek, M. Cornwell, and K. J. Green
Desmoglein 1-dependent suppression of EGFR signaling promotes epidermal differentiation and morphogenesis
J. Cell Biol., June 29, 2009; 185(7): 1243 - 1258.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
P. M. Elias, M. L. Williams, W. M. Holleran, Y. J. Jiang, and M. Schmuth
Thematic review series: Skin Lipids. Pathogenesis of permeability barrier abnormalities in the ichthyoses: inherited disorders of lipid metabolism
J. Lipid Res., April 1, 2008; 49(4): 697 - 714.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
K. S. Suh, M. Mutoh, T. Mutoh, L. Li, A. Ryscavage, J. M. Crutchley, R. A. Dumont, C. Cheng, and S. H. Yuspa
CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation
J. Cell Sci., August 1, 2007; 120(15): 2631 - 2640.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Balasubramanian, L. Zhu, and R. L. Eckert
Apigenin Inhibition of Involucrin Gene Expression Is Associated with a Specific Reduction in Phosphorylation of Protein Kinase C{delta} Tyr311
J. Biol. Chem., November 24, 2006; 281(47): 36162 - 36172.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
M. Maslowska, H. Legakis, F. Assadi, and K. Cianflone
Targeting the signaling pathway of acylation stimulating protein
J. Lipid Res., March 1, 2006; 47(3): 643 - 652.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
L. Li, K. Sampat, N. Hu, J. Zakari, and S. H. Yuspa
Protein Kinase C Negatively Regulates Akt Activity and Modifies UVC-induced Apoptosis in Mouse Keratinocytes
J. Biol. Chem., February 10, 2006; 281(6): 3237 - 3243.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. A. Rambaratsingh, J. C. Stone, P. M. Blumberg, and P. S. Lorenzo
RasGRP1 Represents a Novel Non-protein Kinase C Phorbol Ester Signaling Pathway in Mouse Epidermal Keratinocytes
J. Biol. Chem., December 26, 2003; 278(52): 52792 - 52801.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
C. Cataisson, E. Joseloff, R. Murillas, A. Wang, C. Atwell, S. Torgerson, M. Gerdes, J. Subleski, J.-L. Gao, P. M. Murphy, et al.
Activation of Cutaneous Protein Kinase C{alpha} Induces Keratinocyte Apoptosis and Intraepidermal Inflammation by Independent Signaling Pathways
J. Immunol., September 1, 2003; 171(5): 2703 - 2713.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
B. A. Shapiro, S. Ray, E. Jung, W. T. Allred, and W. B. Bollag
Putative Conventional Protein Kinase C Inhibitor Godecke 6976 [12-(2-Cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole] Stimulates Transglutaminase Activity in Primary Mouse Epidermal Keratinocytes
J. Pharmacol. Exp. Ther., July 1, 2002; 302(1): 352 - 358.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. Deucher, T. Efimova, and R. L. Eckert
Calcium-dependent Involucrin Expression Is Inversely Regulated by Protein Kinase C (PKC){alpha} and PKC{delta}
J. Biol. Chem., May 10, 2002; 277(19): 17032 - 17040.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
E. Joseloff, C. Cataisson, H. Aamodt, H. Ocheni, P. Blumberg, A. J. Kraker, and S. H. Yuspa
Src Family Kinases Phosphorylate Protein Kinase C {delta} on Tyrosine Residues and Modify the Neoplastic Phenotype of Skin Keratinocytes
J. Biol. Chem., April 5, 2002; 277(14): 12318 - 12323.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Kanke, S. R. Macfarlane, M. J. Seatter, E. Davenport, A. Paul, R. C. McKenzie, and R. Plevin
Proteinase-activated Receptor-2-mediated Activation of Stress-activated Protein Kinases and Inhibitory {kappa}B Kinases in NCTC 2544 Keratinocytes
J. Biol. Chem., August 24, 2001; 276(34): 31657 - 31666.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
F. Noya, W.-M. Chien, T. R. Broker, and L. T. Chow
p21cip1 Degradation in Differentiated Keratinocytes Is Abrogated by Costabilization with Cyclin E Induced by Human Papillomavirus E7
J. Virol., July 1, 2001; 75(13): 6121 - 6134.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
A. Alt, M. Ohba, L. Li, M. Gartsbein, A. Belanger, Mitchell. F. Denning, T. Kuroki, S. H. Yuspa, and T. Tennenbaum
Protein Kinase C{{delta}}-mediated Phosphorylation of {{alpha}}6{beta}4 Is Associated with Reduced Integrin Localization to the Hemidesmosome and Decreased Keratinocyte Attachment
Cancer Res., June 1, 2001; 61(11): 4591 - 4598.
[Abstract] [Full Text] [PDF]


Home page
J. Lipid Res.Home page
K. Hanley, L. Wood, D. C. Ng, S. S. He, P. Lau, A. Moser, P. M. Elias, D. D. Bikle, M. L. Williams, and K. R. Feingold
Cholesterol sulfate stimulates involucrin transcription in keratinocytes by increasing Fra-1, Fra-2, and Jun D
J. Lipid Res., March 1, 2001; 42(3): 390 - 398.
[Abstract] [Full Text]


Home page
DiabetesHome page
S. Shen, A. Alt, E. Wertheimer, M. Gartsbein, T. Kuroki, M. Ohba, L. Braiman, S. R. Sampson, and T. Tennenbaum
PKC{delta} Activation: A Divergence Point in the Signaling of Insulin and IGF-1--Induced Proliferation of Skin Keratinocytes
Diabetes, February 1, 2001; 50(2): 255 - 264.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
M. Schmidt, M. Goebeler, G. Posern, S. M. Feller, C. S. Seitz, E.-B. Brocker, U. R. Rapp, and S. Ludwig
Ras-independent Activation of the Raf/MEK/ERK Pathway upon Calcium-induced Differentiation of Keratinocytes
J. Biol. Chem., December 29, 2000; 275(52): 41011 - 41017.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
I. Kronfeld, G. Kazimirsky, P. S. Lorenzo, S. H. Garfield, P. M. Blumberg, and C. Brodie
Phosphorylation of Protein Kinase C{delta} on Distinct Tyrosine Residues Regulates Specific Cellular Functions
J. Biol. Chem., November 10, 2000; 275(45): 35491 - 35498.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
M. A. Otieno and T. W. Kensler
A Role for Protein Kinase C-{{delta}} in the Regulation of Ornithine Decarboxylase Expression by Oxidative Stress
Cancer Res., August 1, 2000; 60(16): 4391 - 4396.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
I. M. Hussaini, L. R. Karns, G. Vinton, J. E. Carpenter, G. T. Redpath, J. J. Sando, and S. R. VandenBerg
Phorbol 12-Myristate 13-Acetate Induces Protein Kinase C{eta}-specific Proliferative Response in Astrocytic Tumor Cells
J. Biol. Chem., July 21, 2000; 275(29): 22348 - 22354.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
P. J. Reddig, N. E. Dreckschmidt, J. Zou, S. E. Bourguignon, T. D. Oberley, and A. K. Verma
Transgenic Mice Overexpressing Protein Kinase C{{epsilon}} in Their Epidermis Exhibit Reduced Papilloma Burden but Enhanced Carcinoma Formation after Tumor Promotion
Cancer Res., February 1, 2000; 60(3): 595 - 602.
[Abstract] [Full Text]


Home page
J. Cell Sci.Home page
M. D. Hines, H. C. Jin, M. J. Wheelock, and P. J. Jensen
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes
J. Cell Sci., December 15, 1999; 112(24): 4569 - 4579.
[Abstract] [PDF]


Home page
Mol. Cell. Biol.Home page
L. Li, P. S. Lorenzo, K. Bogi, P. M. Blumberg, and S. H. Yuspa
Protein Kinase Cdelta Targets Mitochondria, Alters Mitochondrial Membrane Potential, and Induces Apoptosis in Normal and Neoplastic Keratinocytes When Overexpressed by an Adenoviral Vector
Mol. Cell. Biol., December 1, 1999; 19(12): 8547 - 8558.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. D. Griner, F. Qin, E. Jung, C. K. Sue-Ling, K. B. Crawford, R. Mann-Blakeney, R. J. Bollag, and W. B. Bollag
1,25-Dihydroxyvitamin D3 Induces Phospholipase D-1 Expression in Primary Mouse Epidermal Keratinocytes
J. Biol. Chem., February 19, 1999; 274(8): 4663 - 4670.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. F. Denning, Y. Wang, B. J. Nickoloff, and T. Wrone-Smith
Protein Kinase C{delta} Is Activated by Caspase-dependent Proteolysis during Ultraviolet Radiation-induced Apoptosis of Human Keratinocytes
J. Biol. Chem., November 6, 1998; 273(45): 29995 - 30002.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. W. J. Sark, D. F. Fischer, E. de Meijer, P. van de Putte, and C. Backendorf
AP-1 and Ets Transcription Factors Regulate the Expression of the Human SPRR1A Keratinocyte Terminal Differentiation Marker
J. Biol. Chem., September 18, 1998; 273(38): 24683 - 24692.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. D. Jenkins, H. Nakagawa, and A. K. Rustgi
The Keratinocyte-specific Epstein-Barr Virus ED-L2 Promoter Is Regulated by Phorbol 12-Myristate 13-Acetate through Twocis-Regulatory Elements Containing E-box and Kruppel-like Factor Motifs
J. Biol. Chem., September 26, 1997; 272(39): 24433 - 24442.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. G. Thompson, S. C. Mackie, A. Thom, and R. M. Palmer
Regulation of Phospholipase D in L6 Skeletal Muscle Myoblasts: ROLE OF PROTEIN KINASE C AND RELATIONSHIP TO PROTEIN SYNTHESIS
J. Biol. Chem., April 18, 1997; 272(16): 10910 - 10916.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. Franchi-Gazzola, R. Visigalli, O. Bussolati, and G. C. Gazzola
Involvement of Protein Kinase C{epsilon} in the Stimulation of Anionic Amino Acid Transport in Cultured Human Fibroblasts
J. Biol. Chem., October 18, 1996; 271(42): 26124 - 26130.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
J L Brissette, J Li, J Kamimura, D Lee, and G P Dotto
The product of the mouse nude locus, Whn, regulates the balance between epithelial cell growth and differentiation.
Genes & Dev., September 1, 1996; 10(17): 2212 - 2221.
[Abstract] [PDF]


Home page
J Biol ChemHome page
M. F. Denning, A. A. Dlugosz, D. W. Threadgill, T. Magnuson, and S. H. Yuspa
Activation of the Epidermal Growth Factor Receptor Signal Transduction Pathway Stimulates Tyrosine Phosphorylation of Protein Kinase C
J. Biol. Chem., March 8, 1996; 271(10): 5325 - 5331.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
I. M. Hussaini, L. R. Karns, G. Vinton, J. E. Carpenter, G. T. Redpath, J. J. Sando, and S. R. VandenBerg
Phorbol 12-Myristate 13-Acetate Induces Protein Kinase C{eta}-specific Proliferative Response in Astrocytic Tumor Cells
J. Biol. Chem., July 21, 2000; 275(29): 22348 - 22354.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. Schmidt, M. Goebeler, G. Posern, S. M. Feller, C. S. Seitz, E.-B. Brocker, U. R. Rapp, and S. Ludwig
Ras-independent Activation of the Raf/MEK/ERK Pathway upon Calcium-induced Differentiation of Keratinocytes
J. Biol. Chem., December 29, 2000; 275(52): 41011 - 41017.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
I. Kronfeld, G. Kazimirsky, P. S. Lorenzo, S. H. Garfield, P. M. Blumberg, and C. Brodie
Phosphorylation of Protein Kinase C{delta} on Distinct Tyrosine Residues Regulates Specific Cellular Functions
J. Biol. Chem., November 10, 2000; 275(45): 35491 - 35498.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Kanke, S. R. Macfarlane, M. J. Seatter, E. Davenport, A. Paul, R. C. McKenzie, and R. Plevin
Proteinase-activated Receptor-2-mediated Activation of Stress-activated Protein Kinases and Inhibitory {kappa}B Kinases in NCTC 2544 Keratinocytes
J. Biol. Chem., August 24, 2001; 276(34): 31657 - 31666.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.