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Cell Growth & Differentiation, Vol 6, Issue 12 1609-1617, Copyright © 1995 by American Association of Cancer Research

ARTICLES

WT1 expression alters tumorigenicity of the G401 kidney-derived cell line

ML McMaster, M Gessler, EJ Stanbridge and BE Weissman
Department of Pathology, University of North Carolina, Chapel Hill 27599, USA.

Recent studies have implicated a loss of WT1 tumor suppressor gene function in the development of Wilms' tumor (WT). To determine the potential biological consequences of WT1 inactivation in these tumors, we transfected two different splice variant forms of this gene into the pediatric kidney-derived cell line G401. Introduction of this gene caused no detectable effects on the population doubling times of the cell line; proliferative capacity in soft agar was not significantly affected. However, the expression of this gene altered the morphology of the cells in culture and caused a significant suppression of tumorigenicity in the cells. Thus, the expression of WT1 in a pediatric kidney-derived cell line lacking endogenous WT1 production caused demonstrable effects on its in vitro and in vivo growth properties. These data strengthen the concept for a central role for WT1 inactivation in the etiology of this disease.


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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.