CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deweindt, C.
Right arrow Articles by Leprince, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deweindt, C.
Right arrow Articles by Leprince, D.

Cell Growth & Differentiation, Vol 6, Issue 12 1495-1503, Copyright © 1995 by American Association of Cancer Research

ARTICLES

The LAZ3/BCL6 oncogene encodes a sequence-specific transcriptional inhibitor: a novel function for the BTB/POZ domain as an autonomous repressing domain

C Deweindt, O Albagli, F Bernardin, P Dhordain, S Quief, D Lantoine, JP Kerckaert and D Leprince
U124 INSERM, Institut de Recherches Sur le Cancer de Lille, France.

Rearrangements and mutations of the LAZ3/BCL6 gene are the most frequent events associated with diffuse large-cell lymphoma, a particular class of non-Hodgkin's lymphomas. This gene encodes a putative regulatory protein with six COOH-terminal Kruppel-like zinc fingers and a NH2-terminal hydrophobic region, the so-called BTB/POZ domain, which mediates homo- as well as heterotypic interactions in other related proteins. Recently, a consensus binding sequence has been defined using the isolated LAZ3/BCL6 zinc finger region produced in bacteria. To understand the normal and oncogenic functions of LAZ3/BCL6, we examined its properties as a transcription factor. We thus demonstrated that its full-length product binds to the same consensus sequence, although the BTB/POZ domain decreases this activity, at least in vitro. In transient transfection experiments, the LAZ3/BCL6 protein exerts a repressive effect, both as a wild-type protein on its own target sequence and as a GAL-4 fusion protein. Furthermore, our results indicate that the BTB/POZ domain plays a prominent role in the mediation of this activity. Indeed, on the LAZ3/BCL6 cognate sequence, deletion of the BTB/POZ domain diminishes the repressive function. Conversely, as a GAL-4 chimera, the isolated LAZ3/BCL6 BTB/POZ domain appears nearly as efficient as the entire protein at inducing transcriptional repression. Taken together, these findings demonstrate that the LAZ3/BCL6 is a sequence-specific transcriptional repressor and point to a novel function for the BTB/POZ region, at least in LAZ3/BCL6, as an autonomous transcriptional inhibitory domain.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
B. W. Baron, N. Zeleznik-Le, M. J. Baron, C. Theisler, D. Huo, M. D. Krasowski, M. J. Thirman, R. M. Baron, and J. M. Baron
Repression of the PDCD2 gene by BCL6 and the implications for the pathogenesis of human B and T cell lymphomas
PNAS, May 1, 2007; 104(18): 7449 - 7454.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
H. Ichii, A. Sakamoto, M. Arima, M. Hatano, Y. Kuroda, and T. Tokuhisa
Bcl6 is essential for the generation of long-term memory CD4+ T cells
Int. Immunol., April 1, 2007; 19(4): 427 - 433.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A.-J. Rodrigues, G. Coppola, C. Santos, M. d. C. Costa, M. Ailion, J. Sequeiros, D. H. Geschwind, and P. Maciel
Functional genomics and biochemical characterization of the C. elegans orthologue of the Machado-Joseph disease protein ataxin-3
FASEB J, April 1, 2007; 21(4): 1126 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. D. Gearhart, C. M. Corcoran, J. A. Wamstad, and V. J. Bardwell
Polycomb Group and SCF Ubiquitin Ligases Are Found in a Novel BCOR Complex That Is Recruited to BCL6 Targets.
Mol. Cell. Biol., September 1, 2006; 26(18): 6880 - 6889.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
E. Arguni, M. Arima, N. Tsuruoka, A. Sakamoto, M. Hatano, and T. Tokuhisa
JunD/AP-1 and STAT3 are the major enhancer molecules for high Bcl6 expression in germinal center B cells
Int. Immunol., July 1, 2006; 18(7): 1079 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
O. A. O'Connor
Targeting Histones and Proteasomes: New Strategies for the Treatment of Lymphoma
J. Clin. Oncol., September 10, 2005; 23(26): 6429 - 6436.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Pinte, N. Stankovic-Valentin, S. Deltour, B. R. Rood, C. Guerardel, and D. Leprince
The Tumor Suppressor Gene HIC1 (Hypermethylated in Cancer 1) Is a Sequence-specific Transcriptional Repressor: DEFINITION OF ITS CONSENSUS BINDING SEQUENCE AND ANALYSIS OF ITS DNA BINDING AND REPRESSIVE PROPERTIES
J. Biol. Chem., September 10, 2004; 279(37): 38313 - 38324.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Chevallier, C. M. Corcoran, C. Lennon, E. Hyjek, A. Chadburn, V. J. Bardwell, J. D. Licht, and A. Melnick
ETO protein of t(8;21) AML is a corepressor for Bcl-6 B-cell lymphoma oncoprotein
Blood, February 15, 2004; 103(4): 1454 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Lours, O. Bardot, D. Godt, F. A. Laski, and J.-L. Couderc
The Drosophila melanogaster BTB proteins bric a brac bind DNA through a composite DNA binding domain containing a pipsqueak and an AT-Hook motif
Nucleic Acids Res., September 15, 2003; 31(18): 5389 - 5398.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. Takeda, M. Arima, N. Tsuruoka, S. Okada, M. Hatano, A. Sakamoto, Y. Kohno, and T. Tokuhisa
Bcl6 Is a Transcriptional Repressor for the IL-18 Gene
J. Immunol., July 1, 2003; 171(1): 426 - 431.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Sanchez-Beato, A. Sanchez-Aguilera, and M. A. Piris
Cell cycle deregulation in B-cell lymphomas
Blood, February 15, 2003; 101(4): 1220 - 1235.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
A. WINDEMUTH, M. KUMAR, K. NANDABALAN, B. KOSHY, C. XU, M. PUNGLIYA, and R. JUDSON
Genome-wide Association of Haplotype Markers to Gene Expression Levels
Cold Spring Harb Symp Quant Biol, January 1, 2003; 68(0): 89 - 108.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Wang, Z. Li, A. Naganuma, and B. H. Ye
Negative autoregulation of BCL-6 is bypassed by genetic alterations in diffuse large B cell lymphomas
PNAS, November 12, 2002; 99(23): 15018 - 15023.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. P. Butler, S. Iida, D. Capello, D. Rossi, P. H. Rao, P. Nallasivam, D. C. Louie, S. Chaganti, T. Au, R. D. Gascoyne, et al.
Alternative Translocation Breakpoint Cluster Region 5' to BCL-6 in B-cell Non-Hodgkin's Lymphoma
Cancer Res., July 15, 2002; 62(14): 4089 - 4094.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Arima, H. Toyama, H. Ichii, S. Kojima, S. Okada, M. Hatano, G. Cheng, M. Kubo, T. Fukuda, and T. Tokuhisa
A Putative Silencer Element in the IL-5 Gene Recognized by Bcl6
J. Immunol., July 15, 2002; 169(2): 829 - 836.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M.-J. Artiga, A.-I. Saez, C. Romero, M. Sanchez-Beato, M.-S. Mateo, C. Navas, M. Mollejo, and M. A. Piris
A Short Mutational Hot Spot in the First Intron of BCL-6 Is Associated with Increased BCL-6 Expression and with Longer Overall Survival in Large B-Cell Lymphomas
Am. J. Pathol., April 1, 2002; 160(4): 1371 - 1380.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Nili, G. S. Cojocaru, Y. Kalma, D. Ginsberg, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, R. Berger, S. Shaklai, N. Amariglio, et al.
Nuclear membrane protein LAP2{beta} mediates transcriptional repression alone and together with its binding partner GCL (germ-cell-less)
J. Cell Sci., March 11, 2002; 114(18): 3297 - 3307.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Kim, X. Fang, H. Ji, A. F. Paulson, J. M. Daniel, M. Ciesiolka, F. van Roy, and P. D. McCrea
Isolation and Characterization of XKaiso, a Transcriptional Repressor That Associates with the Catenin Xp120ctn in Xenopus laevis
J. Biol. Chem., March 1, 2002; 277(10): 8202 - 8208.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. W. Baron, J. Anastasi, M. J. Thirman, Y. Furukawa, S. Fears, D. C. Kim, F. Simone, M. Birkenbach, A. Montag, A. Sadhu, et al.
The human programmed cell death-2 (PDCD2) gene is a target of BCL6 repression: Implications for a role of BCL6 in the down-regulation of apoptosis
PNAS, February 14, 2002; (2002) 42702599.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. T. Fearon, P. Manders, and S. D. Wagner
Arrested Differentiation, the Self-Renewing Memory Lymphocyte, and Vaccination
Science, July 13, 2001; 293(5528): 248 - 250.
[Abstract] [Full Text]

Home page
 Pharmacol. Rev.Home page
S. C. Dixon, K. B. Knopf, and W. D. Figg
The Control of Prostate-Specific Antigen Expression and Gene Regulation by Pharmacological Agents
Pharmacol. Rev., March 1, 2001; 53(1): 73 - 92.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
R. Reljic, S. D. Wagner, L. J. Peakman, and D. T. Fearon
Suppression of Signal Transducer and Activator of Transcription 3-dependent B Lymphocyte Terminal Differentiation by BCL-6
J. Exp. Med., December 18, 2000; 192(12): 1841 - 1848.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
O. Albagli, C. Lindon, D. Lantoine, S. Quief, E. Puvion, C. Pinset, and F. Puvion-Dutilleul
DNA Replication Progresses on the Periphery of Nuclear Aggregates Formed by the BCL6 Transcription Factor
Mol. Cell. Biol., November 15, 2000; 20(22): 8560 - 8570.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
L. Pasqualucci, A. Neri, L. Baldini, R. Dalla-Favera, and A. Migliazza
BCL-6 Mutations Are Associated with Immunoglobulin Variable Heavy Chain Mutations in B-Cell Chronic Lymphocytic Leukemia
Cancer Res., October 1, 2000; 60(20): 5644 - 5648.
[Abstract] [Full Text]

Home page
 GeneticsHome page
Y. Wen, D. Nguyen, Y. Li, and Z.-C. Lai
The N-terminal BTB/POZ Domain and C-Terminal Sequences Are Essential for Tramtrack69 to Specify Cell Fate in the Developing Drosophila Eye
Genetics, September 1, 2000; 156(1): 195 - 203.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
S. A. Mackler, L. Korutla, X.-Y. Cha, M. J. Koebbe, K. M. Fournier, M. S. Bowers, and P. W. Kalivas
NAC-1 Is a Brain POZ/BTB Protein That Can Prevent Cocaine-Induced Sensitization in the Rat
J. Neurosci., August 15, 2000; 20(16): 6210 - 6217.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. G. Willis and M. J. S. Dyer
The role of immunoglobulin translocations in the pathogenesis of B-cell malignancies
Blood, August 1, 2000; 96(3): 808 - 822.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
K. D. Huynh, W. Fischle, E. Verdin, and V. J. Bardwell
BCoR, a novel corepressor involved in BCL-6 repression
Genes & Dev., July 15, 2000; 14(14): 1810 - 1823.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
T. L. Sander, A. L. Haas, M. J. Peterson, and J. F. Morris
Identification of a Novel SCAN Box-related Protein That Interacts with MZF1B. THE LEUCINE-RICH SCAN BOX MEDIATES HETERO- AND HOMOPROTEIN ASSOCIATIONS
J. Biol. Chem., April 21, 2000; 275(17): 12857 - 12867.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Fedele, G. Benvenuto, R. Pero, B. Majello, S. Battista, F. Lembo, E. Vollono, P. M. Day, M. Santoro, L. Lania, et al.
A Novel Member of the BTB/POZ Family, PATZ, Associates with the RNF4 RING Finger Protein and Acts as a Transcriptional Repressor
J. Biol. Chem., March 10, 2000; 275(11): 7894 - 7901.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Deltour, C. Guerardel, and D. Leprince
Recruitment of SMRT/N-CoR-mSin3A-HDAC-repressing complexes is not a general mechanism for BTB/POZ transcriptional repressors: The case of HIC-1 and gamma FBP-B
PNAS, December 21, 1999; 96(26): 14831 - 14836.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Li, H. Peng, D. C. Schultz, J. M. Lopez-Guisa, F. J. Rauscher III, and R. Marmorstein
Structure-Function Studies of the BTB/POZ Transcriptional Repression Domain from the Promyelocytic Leukemia Zinc Finger Oncoprotein
Cancer Res., October 1, 1999; 59(20): 5275 - 5282.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Kukita, T. Kukita, M. Ouchida, H. Maeda, H. Yatsuki, and O. Kohashi
Osteoclast-Derived Zinc Finger (OCZF) Protein With POZ Domain, a Possible Transcriptional Repressor, Is Involved in Osteoclastogenesis
Blood, September 15, 1999; 94(6): 1987 - 1997.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
T. Yoshida, T. Fukuda, M. Hatano, H. Koseki, S.-i. Okabe, K. Ishibashi, S. Kojima, M. Arima, I. Komuro, G. Ishii, et al.
The role of Bcl6 in mature cardiac myocytes
Cardiovasc Res, June 1, 1999; 42(3): 670 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. M. Daniel and A. B. Reynolds
The Catenin p120ctn Interacts with Kaiso, a Novel BTB/POZ Domain Zinc Finger Transcription Factor
Mol. Cell. Biol., May 1, 1999; 19(5): 3614 - 3623.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C.-M. Tang, J. Westling, and E. Seto
trans Repression of the Human Metallothionein IIA Gene Promoter by PZ120, a Novel 120-Kilodalton Zinc Finger Protein
Mol. Cell. Biol., January 1, 1999; 19(1): 680 - 689.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Cesarman, A. Chadburn, Y.-F. Liu, A. Migliazza, R. Dalla-Favera, and D. M. Knowles
BCL-6 Gene Mutations in Posttransplantation Lymphoproliferative Disorders Predict Response to Therapy and Clinical Outcome
Blood, October 1, 1998; 92(7): 2294 - 2302.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Pasqualucci, A. Migliazza, N. Fracchiolla, C. William, A. Neri, L. Baldini, R. S. K. Chaganti, U. Klein, R. Kuppers, K. Rajewsky, et al.
BCL-6 mutations in normal germinal center B cells: Evidence of somatic hypermutation acting outside Ig loci
PNAS, September 29, 1998; 95(20): 11816 - 11821.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Okabe, T. Fukuda, K. Ishibashi, S. Kojima, S. Okada, M. Hatano, M. Ebara, H. Saisho, and T. Tokuhisa
BAZF, a Novel Bcl6 Homolog, Functions as a Transcriptional Repressor
Mol. Cell. Biol., July 1, 1998; 18(7): 4235 - 4244.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
H. Niu, B. H. Ye, and R. Dalla-Favera
Antigen receptor signaling induces MAP kinase-mediated phosphorylation and degradation of the BCL-6 transcription factor
Genes & Dev., July 1, 1998; 12(13): 1953 - 1961.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Dhordain, O. Albagli, R. J. Lin, S. Ansieau, S. Quief, A. Leutz, J.-P. Kerckaert, R. M. Evans, and D. Leprince
Corepressor SMRT binds the BTB/POZ repressing domain of the LAZ3/BCL6 oncoprotein
PNAS, September 30, 1997; 94(20): 10762 - 10767.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-H. Hong, G. David, C.-W. Wong, A. Dejean, and M. L. Privalsky
SMRT corepressor interacts with PLZF and with the PML-retinoic acid receptor alpha  (RARalpha ) and PLZF-RARalpha oncoproteins associated with acute promyelocytic leukemia
PNAS, August 19, 1997; 94(17): 9028 - 9033.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
T. Fukuda, T. Yoshida, S. Okada, M. Hatano, T. Miki, K. Ishibashi, S. Okabe, H. Koseki, S. Hirosawa, M. Taniguchi, et al.
Disruption of the Bcl6 Gene Results in an Impaired Germinal Center Formation
J. Exp. Med., August 4, 1997; 186(3): 439 - 448.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. W. Baron, J. Anastasi, M. J. Thirman, Y. Furukawa, S. Fears, D. C. Kim, F. Simone, M. Birkenbach, A. Montag, A. Sadhu, et al.
The human programmed cell death-2 (PDCD2) gene is a target of BCL6 repression: Implications for a role of BCL6 in the down-regulation of apoptosis
PNAS, March 5, 2002; 99(5): 2860 - 2865.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.