CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adler, V.
Right arrow Articles by Ronai, Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adler, V.
Right arrow Articles by Ronai, Z.

Cell Growth & Differentiation, Vol 6, Issue 11 1437-1446, Copyright © 1995 by American Association of Cancer Research


ARTICLES

jun-NH2-terminal kinase activation mediated by UV-induced DNA lesions in melanoma and fibroblast cells

V Adler, SY Fuchs, J Kim, A Kraft, MP King, J Pelling and Z Ronai
Molecular Carcinogenesis Program, American Health Foundation, Valhalla, New York 10595, USA.

jun-NH2-terminal kinase (JNK) belongs to a family of protein kinases that phosphorylates c-Jun, ATF2, and Elk1 in response to various forms of stress including UV irradiation and heat shock. Although in previous studies we have demonstrated the importance of membrane components for JNK activation by UV irradiation, here we have elucidated the role of DNA damage in this response. We show that in vitro-irradiated or sonicated DNA that is added to proteins prepared from UV-treated cells can further induce JNK activation in a dose-dependent manner. When compared with UV-B (300 nm), UV-C (254 nm), which is better absorbed by the DNA, is significantly more potent in activating JNK. Furthermore, when wavelengths lower than 300 nm were filtered out, UV-B was no longer able to activate JNK. With the aid of melanoma and fibroblast cells, which exhibit different resistances to irradiation and require different UV doses to generate the same number of DNA lesions, we demonstrate that above a threshold level of 0.45 lesions and up to 0.75 lesions per 1875 bp, the degree of JNK activation correlates with the amount of lesions induced by UV-C irradiation. Finally, to explore the role of nuclear and mitochondrial DNA (mtDNA) in mediating JNK activation after UV irradiation, we have used cells that lacks mtDNA. Although the lack of mtDNA did not impair the ability of UV to activate JNK, when enucleated, these cells had lost the ability to activate JNK in response to UV irradiation. Overall, our results suggest that DNA damage in the nuclear compartment is an essential component that acts in concert with membrane-anchored proteins to mediate c-Jun phosphorylation by JNK.


This article has been cited by other articles:


Home page
FASEB J.Home page
E. Harja, J. S. Chang, Y. Lu, M. Leitges, Y. S. Zou, A. M. Schmidt, and S.-F. Yan
Mice deficient in PKC{beta} and apolipoprotein E display decreased atherosclerosis
FASEB J, April 1, 2009; 23(4): 1081 - 1091.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
P. A. Gillis, L. H. Okagaki, and S. A. Rice
Herpes Simplex Virus Type 1 ICP27 Induces p38 Mitogen-Activated Protein Kinase Signaling and Apoptosis in HeLa Cells
J. Virol., February 15, 2009; 83(4): 1767 - 1777.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
D. Kitagawa, H. Kajiho, T. Negishi, S. Ura, T. Watanabe, T. Wada, H. Ichijo, T. Katada, and H. Nishina
Release of RASSF1C from the nucleus by Daxx degradation links DNA damage and SAPK/JNK activation
EMBO J., July 26, 2006; 25(14): 3286 - 3297.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
T. Tonini, C. Gabellini, L. Bagella, G. D'Andrilli, V. Masciullo, G. Romano, G. Scambia, G. Zupi, and A. Giordano
pRb2/p130 Decreases Sensitivity to Apoptosis Induced by Camptothecin and Doxorubicin but not by Taxol
Clin. Cancer Res., December 1, 2004; 10(23): 8085 - 8093.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
G. Mathonnet, C. Leger, J. Desnoyers, R. Drouin, J.-P. Therrien, and E. A. Drobetsky
UV wavelength-dependent regulation of transcription-coupled nucleotide excision repair in p53-deficient human cells
PNAS, June 10, 2003; 100(12): 7219 - 7224.
[Abstract] [Full Text] [PDF]


Home page
Sci SignalHome page
A. M. Bode and Z. Dong
Mitogen-Activated Protein Kinase Activation in UV-Induced Signal Transduction
Sci. Signal., January 28, 2003; 2003(167): re2 - re2.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
A. E. Lawson, H. Bao, A. Wickrema, S. M. Jacobs-Helber, and S. T. Sawyer
Phosphatase inhibition promotes antiapoptotic but not proliferative signaling pathways in erythropoietin-dependent HCD57 cells
Blood, September 15, 2000; 96(6): 2084 - 2092.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Buschmann, Z. Yin, A. Bhoumik, and Z. Ronai
Amino-terminal-derived JNK Fragment Alters Expression and Activity of c-Jun, ATF2, and p53 and Increases H2O2-induced Cell Death
J. Biol. Chem., June 2, 2000; 275(22): 16590 - 16596.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
C. Blattner, P. Kannouche, M. Litfin, K. Bender, H. J. Rahmsdorf, J. F. Angulo, and P. Herrlich
UV-Induced Stabilization of c-fos and Other Short-Lived mRNAs
Mol. Cell. Biol., May 15, 2000; 20(10): 3616 - 3625.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
C. Huang, P. Mattjus, W.-y. Ma, M. Rincon, N.-y. Chen, R. E. Brown, and Z. Dong
Involvement of Nuclear Factor of Activated T Cells Activation in UV Response: EVIDENCE FROM CELL CULTURE AND TRANSGENIC MICE
J. Biol. Chem., March 31, 2000; 275(13): 9143 - 9149.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. Hayakawa, M. Ohmichi, H. Kurachi, H. Ikegami, A. Kimura, T. Matsuoka, H. Jikihara, D. Mercola, and Y. Murata
Inhibition of Extracellular Signal-regulated Protein Kinase or c-Jun N-terminal Protein Kinase Cascade, Differentially Activated by Cisplatin, Sensitizes Human Ovarian Cancer Cell Line
J. Biol. Chem., October 29, 1999; 274(44): 31648 - 31654.
[Abstract] [Full Text] [PDF]


Home page
Cell Growth Differ.Home page
R. A. Gjerset, S. Lebedeva, A. Haghighi, S. T. Turla, and D. Mercola
Inhibition of the Jun Kinase Pathway Blocks DNA Repair, Enhances p53-mediated Apoptosis and Promotes Gene Amplification
Cell Growth Differ., August 1, 1999; 10(8): 545 - 554.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
C. Huang, W.-Y. Ma, A. Maxiner, Y. Sun, and Z. Dong
p38 Kinase Mediates UV-induced Phosphorylation of p53 Protein at Serine 389
J. Biol. Chem., April 30, 1999; 274(18): 12229 - 12235.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
V. Adler, Z. Yin, S. Y. Fuchs, M. Benezra, L. Rosario, K. D. Tew, M. R. Pincus, M. Sardana, C. J. Henderson, C. R. Wolf, et al.
Regulation of JNK signaling by GSTp
EMBO J., March 1, 1999; 18(5): 1321 - 1334.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
F. Miralles, M. Parra, C. Caelles, Y. Nagamine, J. Felez, and P. Munoz-Canoves
UV Irradiation Induces the Murine Urokinase-Type Plasminogen Activator Gene via the c-Jun N-Terminal Kinase Signaling Pathway: Requirement of an AP1 Enhancer Element
Mol. Cell. Biol., August 1, 1998; 18(8): 4537 - 4547.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
S. M. Jacobs-Helber, A. Wickrema, M. J. Birrer, and S. T. Sawyer
AP1 Regulation of Proliferation and Initiation of Apoptosis in Erythropoietin-Dependent Erythroid Cells
Mol. Cell. Biol., July 1, 1998; 18(7): 3699 - 3707.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Y. Fuchs, B. Xie, V. Adler, V. A. Fried, R. J. Davis, and Z. Ronai
c-Jun NH2-terminal Kinases Target the Ubiquitination of Their Associated Transcription Factors
J. Biol. Chem., December 19, 1997; 272(51): 32163 - 32168.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. L. Smith and A. J. Fornace Jr.
p53-mediated protective responses to UV irradiation
PNAS, November 11, 1997; 94(23): 12255 - 12257.
[Full Text] [PDF]


Home page
J Biol ChemHome page
O. Potapova, A. Haghighi, F. Bost, C. Liu, M. J. Birrer, R. Gjerset, and D. Mercola
The Jun Kinase/Stress-activated Protein Kinase Pathway Functions to Regulate DNA Repair and Inhibition of the Pathway Sensitizes Tumor Cells to Cisplatin
J. Biol. Chem., May 30, 1997; 272(22): 14041 - 14044.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
X.-L. Cui and J. G. Douglas
Arachidonic acid activates c-jun N-terminal kinase through NADPH oxidase in rabbit proximal tubular epithelial cells
PNAS, April 15, 1997; 94(8): 3771 - 3776.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
P. Pandey, J. Raingeaud, M. Kaneki, R. Weichselbaum, R. J. Davis, D. Kufe, and S. Kharbanda
Activation of p38 Mitogen-activated Protein Kinase by c-Abl-dependent and -independent Mechanisms
J. Biol. Chem., September 27, 1996; 271(39): 23775 - 23779.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
V. Adler, M. R. Pincus, A. Polotskaya, X. Montano, F. K. Friedman, and Z. Ronai
Activation of c-Jun-NH2-Kinase by UV Irradiation Is Dependent on p21ras
J. Biol. Chem., September 20, 1996; 271(38): 23304 - 23309.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Buschmann, Z. Yin, A. Bhoumik, and Z. Ronai
Amino-terminal-derived JNK Fragment Alters Expression and Activity of c-Jun, ATF2, and p53 and Increases H2O2-induced Cell Death
J. Biol. Chem., June 2, 2000; 275(22): 16590 - 16596.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.