CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiess, M.
Right arrow Articles by Hamel, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiess, M.
Right arrow Articles by Hamel, P. A.

Cell Growth & Differentiation, Vol 6, Issue 10 1287-1298, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Expression and activity of the retinoblastoma protein (pRB)-family proteins, p107 and p130, during L6 myoblast differentiation

M Kiess, RM Gill and PA Hamel
Department of Cellular and Molecular Pathology, University of Toronto, Ontario, Canada.

The activity of the E2 F-family of transcription factors is tightly linked to control of the cell cycle. p107 and p130, two closely related members of the retinoblastoma protein-family of negative cell cycle regulators, modulate the activity of the E2f-family proteins by direct interaction with these factors. To understand the role of p107 and p130 in progression through or exit from the cell cycle, we have characterized the expression, phosphorylation state, cyclin-binding, and E2f-binding activity of p107 and p130 during terminal differentiation of rat myoblast cells into immature skeletal muscle (myotubes). In exponentially growing L6 myoblasts, p107 is phosphorylated in a cell cycle-dependent manner, and E2f-site binding complexes containing p107 is phosphorylated in a cell cycle-dependent manner, and E2f-site binding complexes containing p107 can be observed throughout the cell cycle. During differentiation of L6 cells, p107 levels are reduced, while p130 protein levels are increased 8-fold. Despite both p107 and p130 becoming hypophosphorylated during myogenesis, the E2F-site DNA-binding complexes containing p107 observed in exponentially growing myoblasts are quantitatively replaced in myotubes with complexes containing only p130. In myotubes, p107 is not associated with E2f-family proteins that are capable of binding DNA. The failure to observe p107-containing complexes in myotubes appears to be due to the differentiation-specific induction of both p130 and cyclin D3, p107 is found in complexes with cyclin D3 in myotubes, and the addition of exogenous cyclin D3 or p130 to lysates from undifferentiated L6 cells was able to disrupt p107-containing E2F-site binding complexes. In myotubes, p130 also forms complexes with cyclin D3 as well as cyclin E, cdk2, and cdk4. We are able to copurify cyclin D3 with cyclin E from myotubes, indicating the presence of a macromolecular complex containing both cyclin E and cyclin D3 simultaneously bound to p130. Thus, in myoblasts, p107 is normally involved in regulation of E2f-family proteins during cell cycle progression, while p130 is a differentiation-specific regulator of E2f activity. Our results also provide evidence that the apparent positive regulator of cell cycle progression, cyclin D3, has a function in terminally differentiated muscle cells.


This article has been cited by other articles:


Home page
Mol. Cell. Biol.Home page
A. Cerezo, M. C. Guadamillas, J. G. Goetz, S. Sanchez-Perales, E. Klein, R. K. Assoian, and M. A. del Pozo
The Absence of Caveolin-1 Increases Proliferation and Anchorage- Independent Growth by a Rac-Dependent, Erk-Independent Mechanism
Mol. Cell. Biol., September 15, 2009; 29(18): 5046 - 5059.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. Parakati and J. X. DiMario
Dynamic Transcriptional Regulatory Complexes, Including E2F4, p107, p130, and Sp1, Control Fibroblast Growth Factor Receptor 1 Gene Expression during Myogenesis
J. Biol. Chem., June 3, 2005; 280(22): 21284 - 21294.
[Abstract] [Full Text] [PDF]


Home page
Sci SignalHome page
N. Olashaw and W. J. Pledger
Paradigms of Growth Control: Relation to Cdk Activation
Sci. Signal., May 28, 2002; 2002(134): re7 - re7.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
N. A. Timchenko, P. Iakova, Z.-J. Cai, J. R. Smith, and L. T. Timchenko
Molecular Basis for Impaired Muscle Differentiation in Myotonic Dystrophy
Mol. Cell. Biol., October 15, 2001; 21(20): 6927 - 6938.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Murai, Y. Nakagawa, H. Maeda, and K. Terada
Altered Regulation of Cell Cycle Machinery Involved in Interleukin-1-induced G1 and G2 Phase Growth Arrest of A375S2 Human Melanoma Cells
J. Biol. Chem., March 2, 2001; 276(9): 6797 - 6806.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
J. W. Harbour and D. C. Dean
The Rb/E2F pathway: expanding roles and emerging paradigms
Genes & Dev., October 1, 2000; 14(19): 2393 - 2409.
[Full Text]


Home page
Clin. Cancer Res.Home page
Q. M. Wang, R. Feinman, F. Kashanchi, J. M. Houghton, G. P. Studzinski, and L. E. Harrison
Changes in E2F Binding after Phenylbutyrate-induced Differentiation of Caco-2 Colon Cancer Cells
Clin. Cancer Res., July 1, 2000; 6(7): 2951 - 2958.
[Abstract] [Full Text]


Home page
Genes Dev.Home page
S. L. Chen, D. H. Dowhan, B. M. Hosking, and G. E.O. Muscat
The steroid receptor coactivator, GRIP-1, is necessary for MEF-2C-dependent gene expression and skeletal muscle differentiation
Genes & Dev., May 15, 2000; 14(10): 1209 - 1228.
[Abstract] [Full Text]


Home page
IOVSHome page
C. M. Francesconi, A. E. K. Hutcheon, E.-H. Chung, A. C. Dalbone, N. C. Joyce, and J. D. Zieske
Expression Patterns of Retinoblastoma and E2F Family Proteins during Corneal Development
, April 1, 2000; 41(5): 1054 - 1062.
[Abstract] [Full Text]


Home page
JCBHome page
R. M. Gill and P. A. Hamel
Subcellular Compartmentalization of E2f Family Members Is Required for Maintenance of the Postmitotic State in Terminally Differentiated Muscle
J. Cell Biol., March 20, 2000; 148(6): 1187 - 1202.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
Q. Ding, Q. Wang, Z. Dong, and B. M. Evers
Characterization and regulation of E2F activity during Caco-2 cell differentiation
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C110 - C117.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
C. Cenciarelli, F. De Santa, P. L. Puri, E. Mattei, L. Ricci, F. Bucci, A. Felsani, and M. Caruso
Critical Role Played by Cyclin D3 in the MyoD-Mediated Arrest of Cell Cycle during Myoblast Differentiation
Mol. Cell. Biol., July 1, 1999; 19(7): 5203 - 5217.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
K. Smith-McCune, D. Kalman, C. Robbins, S. Shivakumar, L. Yuschenkoff, and J. M. Bishop
Intranuclear localization of human papillomavirus 16 E7 during transformation and preferential binding of E7 to the Rb family member p130
PNAS, June 8, 1999; 96(12): 6999 - 7004.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Heart Circ. Physiol.Home page
N. Saadane, L. Alpert, and L. E. Chalifour
TAFII250, Egr-1, and D-type cyclin expression in mice and neonatal rat cardiomyocytes treated with doxorubicin
Am J Physiol Heart Circ Physiol, March 1, 1999; 276(3): H803 - H814.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
B. L. Harvat, A. Wang, P. Seth, and A. M. Jetten
Up-regulation of p27Kip1, p21WAF1/Cip1 and p16Ink4a is associated with, but not sufficient for, induction of squamous differentiation
J. Cell Sci., May 1, 1998; 111(9): 1185 - 1196.
[Abstract] [PDF]


Home page
J Biol ChemHome page
A. De Luca, T. K. MacLachlan, L. Bagella, C. Dean, C. M. Howard, P. P. Claudio, A. Baldi, K. Khalili, and A. Giordano
A Unique Domain of pRb2/p130 Acts as an Inhibitor of Cdk2 Kinase Activity
J. Biol. Chem., August 22, 1997; 272(34): 20971 - 20974.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
K. Helin, K. Holm, A. Niebuhr, H. Eiberg, N. Tommerup, S. Hougaard, H. S. Poulsen, M. Spang-Thomsen, and P. Norgaard
Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma
PNAS, June 24, 1997; 94(13): 6933 - 6938.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
V. M. Richon, R. E. Lyle, and R. E. McGehee Jr.
Regulation and Expression of Retinoblastoma Proteins p107 and p130 during 3T3-L1 Adipocyte Differentiation
J. Biol. Chem., April 11, 1997; 272(15): 10117 - 10124.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
P. L. Puri, M. L. Avantaggiati, C. Balsano, N. Sang, A. Graessmann, A. Giordano, and M. Levrero
p300 is required for MyoD-dependent cell cycle arrest and muscle-specific gene transcription
EMBO J., January 15, 1997; 16(2): 369 - 383.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Murai, Y. Nakagawa, H. Maeda, and K. Terada
Altered Regulation of Cell Cycle Machinery Involved in Interleukin-1-induced G1 and G2 Phase Growth Arrest of A375S2 Human Melanoma Cells
J. Biol. Chem., March 2, 2001; 276(9): 6797 - 6806.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.