CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Noguchi, K.
Right arrow Articles by Tsuruo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Noguchi, K.
Right arrow Articles by Tsuruo, T.

Cell Growth & Differentiation, Vol 6, Issue 10 1271-1277, Copyright © 1995 by American Association of Cancer Research


ARTICLES

A recessive mutant of the U937 cell line acquired resistance to anti-Fas and anti-p55 tumor necrosis factor receptor antibody-induced apoptosis

K Noguchi, M Naito, S Kataoka, S Yonehara and T Tsuruo
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.

Human monocytic leukemia U937 cells readily undergo apoptosis when cells are treated with various stimuli including antitumor agents, tumor necrosis factor (TNF)-alpha and anti-Fas antibody. However, the signal transduction mechanism resulting in apoptosis is unclear. To study the mechanism of apoptosis, we isolated and characterized a mutant, UK110, from U937 cells, which was resistant to TNF-alpha and anti-Fas antibody-induced apoptosis but was less resistant to etoposide-induced apoptosis. TNF-alpha induced signals are mediated by two types of TNF receptors (TNFR), p55- and p75-TNFR, and p55-TNFR is homologous to the Fas antigen. Interestingly, UK110 cells showed resistance to apoptosis by agonistic anti-p55-TNFR antibody, indicating that UK110 cells were resistant to Fas- and p55-TNFR-mediated apoptosis. Because expression of apoptosis-associated molecules, such as c-Myc, Bcl-2, and Bax, was similar between U937 and UK110 cells an undetermined pathway for apoptosis through Fas and p55-TNFR could be mutated in UK110 cells. To clarify the genetic phenotype of UK110 cells, we performed somatic cell hybridization with parental U937 and the UK110 cells. All of the hybrid clones were as sensitive as the parental U937 cells to apoptosis by both anti-Fas and anti-p55-TNFR antibodies, indicating that the apoptosis resistance in UK110 cells resulted from recessive genotype.


This article has been cited by other articles:


Home page
Infect. Immun.Home page
T. Suzuki, M. Kobayashi, K. Isatsu, T. Nishihara, T. Aiuchi, K. Nakaya, and K. Hasegawa
Mechanisms Involved in Apoptosis of Human Macrophages Induced by Lipopolysaccharide from Actinobacillus actinomycetemcomitans in the Presence of Cycloheximide
Infect. Immun., April 1, 2004; 72(4): 1856 - 1865.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
C. Munoz-Pinedo, C. Ruiz-Ruiz, C. Ruiz de Almodovar, C. Palacios, and A. Lopez-Rivas
Inhibition of Glucose Metabolism Sensitizes Tumor Cells to Death Receptor-triggered Apoptosis through Enhancement of Death-inducing Signaling Complex Formation and Apical Procaspase-8 Processing
J. Biol. Chem., April 11, 2003; 278(15): 12759 - 12768.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Varela, C. Munoz-Pinedo, C. Ruiz-Ruiz, G. Robledo, M. Pedroso, and A. Lopez-Rivas
Interferon-{gamma} Sensitizes Human Myeloid Leukemia Cells to Death Receptor-mediated Apoptosis by a Pleiotropic Mechanism
J. Biol. Chem., May 25, 2001; 276(21): 17779 - 17787.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
H. Sakamoto, T. Mashima, A. Kizaki, S. Dan, Y. Hashimoto, M. Naito, and T. Tsuruo
Glyoxalase I is involved in resistance of human leukemia cells to antitumor agent-induced apoptosis
Blood, May 15, 2000; 95(10): 3214 - 3218.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
L. Gabriele, J. Phung, J. Fukumoto, D. Segal, I-M. Wang, P. Giannakakou, N. A. Giese, K. Ozato, and H. C. Morse III
Regulation of Apoptosis in Myeloid Cells by Interferon Consensus Sequence-Binding Protein
J. Exp. Med., August 2, 1999; 190(3): 411 - 422.
[Abstract] [Full Text] [PDF]


Home page
GutHome page
J Shibata, H Goto, T Arisawa, Y Niwa, T Hayakawa, A Nakayama, and N Mori
Regulation of tumour necrosis factor (TNF) induced apoptosis by soluble TNF receptors in Helicobacter pylori infection
Gut, July 1, 1999; 45(1): 24 - 31.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
B. Rayet, J.-A. Lopez-Guerrero, J. Rommelaere, and C. Dinsart
Induction of Programmed Cell Death by Parvovirus H-1 in U937 Cells: Connection with the Tumor Necrosis Factor Alpha Signalling Pathway
J. Virol., November 1, 1998; 72(11): 8893 - 8903.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
R. Caricchio, E. A. Reap, and P. L. Cohen
Fas/Fas Ligand Interactions Are Involved in Ultraviolet-B-Induced Human Lymphocyte Apoptosis
J. Immunol., July 1, 1998; 161(1): 241 - 251.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Torii, M. Naito, and T. Tsuruo
Apoxin I, a Novel Apoptosis-inducing Factor with L-Amino Acid Oxidase Activity Purified from Western Diamondback Rattlesnake Venom
J. Biol. Chem., April 4, 1997; 272(14): 9539 - 9542.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. Naito, K. Nagashima, T. Mashima, and T. Tsuruo
Phosphatidylserine Externalization Is a Downstream Event of Interleukin-1beta -Converting Enzyme Family Protease Activation During Apoptosis
Blood, March 15, 1997; 89(6): 2060 - 2066.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Varela, C. Munoz-Pinedo, C. Ruiz-Ruiz, G. Robledo, M. Pedroso, and A. Lopez-Rivas
Interferon-{gamma} Sensitizes Human Myeloid Leukemia Cells to Death Receptor-mediated Apoptosis by a Pleiotropic Mechanism
J. Biol. Chem., May 25, 2001; 276(21): 17779 - 17787.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.