CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Agrawal, D.
Right arrow Articles by Pledger, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Agrawal, D.
Right arrow Articles by Pledger, W. J.

Cell Growth & Differentiation, Vol 6, Issue 10 1199-1205, Copyright © 1995 by American Association of Cancer Research


ARTICLES

Regulation of cyclin E and p27kip during mitosis in BALB/c 3T3 cells

D Agrawal, F Dong, YZ Wang, D Kayda and WJ Pledger
H. Lee Moffitt Cancer Center and Research Institute, University of South Florida College of Medicine, Tampa 33612, USA.

We have examined the level of cyclin E, a G1 cyclin, and p27kip, a cyclin-dependent kinase inhibitor, in BALB/c 3T3 cells. Cell populations stimulated to undergo cell cycle traverse displayed little change in the level of cyclin E, while p27kip was found to be reduced 50-80% in proliferating cells. Analysis of mitotic cells, however, revealed that cyclin E is virtually absent and begins to reaccumulate soon after mitosis is complete, as does p27kip. Immunoprecipitation experiments revealed that p27kip is associated with cyclin E in quiescent but not proliferating cells, indicating that it may function to prevent development of cyclin E activity in the absence of growth factors. Based on our characterization of cyclin E and p27kip in BALB/c 3T3 cells during progression of the cell cycle, we propose a model of growth regulation controlling the G1 phase of growing and quiescent cells involving mitotic degradation of cyclin E and recovery of p27kip inhibitory activity in a late or postmitotic interval.


This article has been cited by other articles:


Home page
Am. J. Physiol. Gastrointest. Liver Physiol.Home page
G. P. Ilyin, D. Glaise, D. Gilot, G. Baffet, and C. Guguen-Guillouzo
Regulation and role of p21 and p27 cyclin-dependent kinase inhibitors during hepatocyte differentiation and growth
Am J Physiol Gastrointest Liver Physiol, July 1, 2003; 285(1): G115 - G127.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
X. Zhang, W. Wharton, M. Donovan, D. Coppola, R. Croxton, W. D. Cress, and W. J. Pledger
Density-dependent Growth Inhibition of Fibroblasts Ectopically Expressing p27kip1
Mol. Biol. Cell, June 1, 2000; 11(6): 2117 - 2130.
[Abstract] [Full Text]


Home page
Cancer Res.Home page
H. Drissi, D. Hushka, F. Aslam, Q. Nguyen, E. Buffone, A. Koff, A. J. van Wijnen, J. B. Lian, J. L. Stein, and G. S. Stein
The Cell Cycle Regulator p27kip1 Contributes to Growth and Differentiation of Osteoblasts
Cancer Res., August 1, 1999; 59(15): 3705 - 3711.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. W. Ashton, G. Watanabe, C. Albanese, E. O. Harrington, J. A. Ware, and R. G. Pestell
Protein Kinase C{delta} Inhibition of S-Phase Transition in Capillary Endothelial Cells Involves the Cyclin-dependent Kinase Inhibitor p27Kip1
J. Biol. Chem., July 23, 1999; 274(30): 20805 - 20811.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
F. Dong, D. Agrawal, T. Bagui, and W.J. Pledger
Cyclin D3-associated Kinase Activity Is Regulated by p27kip1 in BALB/c 3T3 Cells
Mol. Biol. Cell, August 1, 1998; 9(8): 2081 - 2092.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
F. Dong, W. D. Cress Jr., D. Agrawal, and W. J. Pledger
The Role of Cyclin D3-dependent Kinase in the Phosphorylation of p130 in Mouse BALB/c 3T3 Fibroblasts
J. Biol. Chem., March 13, 1998; 273(11): 6190 - 6195.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
E. R. Fernandes, J. Y. Zhang, and R. J. Rooney
Adenovirus E1A-Regulated Transcription Factor p120E4F Inhibits Cell Growth and Induces the Stabilization of the cdk Inhibitor p21WAF1
Mol. Cell. Biol., January 1, 1998; 18(1): 459 - 467.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1995 by the American Association of Cancer Research.