CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kindas-Mugge, I.
Right arrow Articles by Trautinger, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kindas-Mugge, I.
Right arrow Articles by Trautinger, F.

Cell Growth & Differentiation, Vol 5, Issue 7 777-781, Copyright © 1994 by American Association of Cancer Research


ARTICLES

Increased expression of the M(r) 27,000 heat shock protein (hsp27) in in vitro differentiated normal human keratinocytes

I Kindas-Mugge and F Trautinger
Institute of Tumorbiology, Cancer Research, University of Vienna, Austria.

The M(r) 27,000 heat shock protein (hsp27) is a member of the small heat shock protein family. Cell differentiation is a process in which a role for small heat shock proteins has been suggested. The ability to control the state of differentiation in normal human keratinocytes by modification of extracellular calcium concentration makes it an ideal in vitro system for exploration of the specific components and steps in differentiation. We have focused on the in vitro expression of hsp27 in undifferentiated and differentiated human normal keratinocytes (HNK) as a marker for differentiation. Immunological methods (immunohistochemistry and immunoblotting) as well as Northern blotting were used. Cells of the breast cancer line MCF-7 served as a positive control. We demonstrated that hsp27 was expressed at low levels in normal human keratinocytes, kept under calcium concentrations where cells formed discrete colonies of undifferentiated, noncornified cuboidal cells (0.03 mM Ca2+), and linked cuboidal cells with a noncornified appearance (0.15 mM Ca2+). Upon cultivation in high calcium (1.00 mM Ca2+) where a more morphological state of differentiation was reached, more spindle shaped with cornification of individual cells, a 2-fold increase in hsp27 expression was observed. A somewhat weaker increase in hsp27 mRNA was shown by Northern blot analysis. Our studies provide evidence that hsp27 is accumulated in a differentiation-dependent manner in human normal keratinocytes grown under conditions inducing terminal differentiation (0.03-1.00 mM Ca2+). Therefore, hsp27 can be regarded as a marker of differentiation in human normal keratinocytes.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1994 by the American Association of Cancer Research.