CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, B.
Right arrow Articles by Rosen, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, B.
Right arrow Articles by Rosen, J. M.

Cell Growth & Differentiation, Vol 5, Issue 7 711-721, Copyright © 1994 by American Association of Cancer Research


ARTICLES

Preferential overexpression of a 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development

B Li, N Greenberg, LC Stephens, R Meyn, D Medina and JM Rosen
Department of Cell Biology, Baylor College of Medicine, Houston, Texas.

Regulatory sequences derived from the rat whey acidic protein gene have been used to preferentially overexpress a murine 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice. Several different lines of mice expressing the 172Arg-->Leu mutant p53 displayed an impaired ability to lactate, and the mice expressing the highest levels of mutant p53 were unable to nurse their young. This failure was related to the inhibition of normal lobuloalveolar development that occurred during late pregnancy and a marked decrease in milk protein gene expression at early lactation. Interestingly, immunohistochemical analysis revealed that the mutant p53 was localized predominantly in the cytoplasm of alveolar cells. Ductal development was not overtly impaired in these mice. Expression of the 172Arg-->Leu mutant p53 resulted in radiation-induced apoptosis, and transactivation or repression of the expression of a number of genes, including mdm-2 and proliferating cell nuclear antigen, known properties of wild-type p53. The availability of lines of mice preferentially expressing specific p53 mutants in the mammary gland should facilitate evaluation of the roles of other factors, such as hormones, oncogenes and chemical carcinogens, in the etiology of breast cancer.


This article has been cited by other articles:


Home page
Cold Spring Harb. Perspect. Biol.Home page
A. D. Borowsky
Choosing a Mouse Model: Experimental Biology in Context--The Utility and Limitations of Mouse Models of Breast Cancer
Cold Spring Harb Perspect Biol, September 1, 2011; 3(9): a009670 - a009670.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
I. Hernandez, L. A. Maddison, Y. Wei, F. DeMayo, T. Petras, B. Li, J. R. Gingrich, J. M. Rosen, and N. M. Greenberg
Prostate-Specific Expression of p53R172L Differentially Regulates p21, Bax, and mdm2 to Inhibit Prostate Cancer Progression and Prolong Survival
Mol. Cancer Res., December 1, 2003; 1(14): 1036 - 1047.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
S. B. Tepera, P. D. McCrea, and J. M. Rosen
A {beta}-catenin survival signal is required for normal lobular development in the mammary gland
J. Cell Sci., March 15, 2003; 116(6): 1137 - 1149.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
C. A. Zahnow, R. D. Cardiff, R. Laucirica, D. Medina, and J. M. Rosen
A Role for CCAAT/Enhancer Binding Protein {beta}-Liver-enriched Inhibitory Protein in Mammary Epithelial Cell Proliferation
Cancer Res., January 1, 2001; 61(1): 261 - 269.
[Abstract] [Full Text] [PDF]


Home page
FASEB J.Home page
C. ALBANESE, A. T. REUTENS, B. BOUZAHZAH, M. FU, M. D'AMICO, T. LINK, R. NICHOLSON, R. A. DEPINHO, and R. G. PESTELL
Sustained mammary gland-directed, ponasterone A-inducible expression in transgenic mice
FASEB J, May 1, 2000; 14(7): 877 - 884.
[Abstract] [Full Text]


Home page
BloodHome page
J. C. Byrd, C. Shinn, J. K. Waselenko, E. J. Fuchs, T. A. Lehman, P. L. Nguyen, I. W. Flinn, L. F. Diehl, E. Sausville, and M. R. Grever
Flavopiridol Induces Apoptosis in Chronic Lymphocytic Leukemia Cells Via Activation of Caspase-3 Without Evidence of bcl-2 Modulation or Dependence on Functional p53
Blood, November 15, 1998; 92(10): 3804 - 3816.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1994 by the American Association of Cancer Research.