Cell Growth & Differentiation, Vol 5, Issue 6 557-561, Copyright © 1994 by American Association of Cancer Research

ARTICLES

Constitutive expression of the putative transcription factor SCL associated with proviral insertion in the myeloid leukemic cell line WEHI-3BD-

T Tanigawa, L Robb, AR Green and CG Begley
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.

The murine myeloid leukemia WEHI-3B contains an intracisternal A-particle insertion in a growth factor gene (IL-3) and a homeobox gene (Hox 2.4). Southern blot analysis of the differentiation unresponsive subline WEHI-3BD- demonstrated a rearrangement of the SCL gene, a gene that encodes a protein of the helix-loop-helix class of transcription factors. Northern blot analysis demonstrated an abnormal SCL transcript in the WEHI-3BD- cells, although SCL expression was not detected in the parental cells. The rearrangement was cloned and confirmed as an insertion of an intracisternal A-particle in the 3' untranslated region of the SCL gene. Although SCL is implicated in up to 25% of human T-cell acute lymphoblastic leukemia, this is the first example of SCL involvement in a myeloid leukemia.

This article has been cited by other articles:

				Y. Zhang, K. J. Payne, Y. Zhu, M. A. Price, Y. K. Parrish, E. Zielinska, L. W. Barsky, and G. M. Crooks SCL Expression at Critical Points in Human Hematopoietic Lineage Commitment Stem Cells, June 1, 2005; 23(6): 852 - 860. [Abstract] [Full Text] [PDF]

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