Cell Growth & Differentiation, Vol 5, Issue 4 419-429, Copyright © 1994 by American Association of Cancer Research

ARTICLES

Bcl-2 expressed using a retroviral vector is localized primarily in the nuclear membrane and the endoplasmic reticulum of chicken embryo fibroblasts

I Givol, I Tsarfaty, J Resau, S Rulong, PP da Silva, G Nasioulas, J DuHadaway, SH Hughes and DL Ewert
ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201.

A complementary DNA for human bcl-2 was cloned into the replication competent avian retrovirus vector RCASBP, and the resulting virus was used to express human Bcl-2 protein at high levels in chicken embryo fibroblasts. The expression of Bcl-2 did not transform or significantly alter the longevity of the chicken embryo fibroblasts in the presence of normal amounts of serum. However, the expression of Bcl-2 blocked c-Myc-induced apoptosis in these cells. Fractionation of the infected chicken embryo fibroblasts indicated that the protein was distributed equally between nuclear and high density cytoplasmic membranes. Immunofluorescence analysis by confocal microscopy and immunoelectron microscopy showed that the Bcl-2 protein was primarily associated with the nuclear membrane and with the endoplasmic reticulum. Reduced amounts of the protein were associated with other membranes in the cytoplasm. These data show that, in this system, the Bcl-2 protein associates with the nuclear membrane and intracytoplasmic membranes but is not preferentially associated with mitochondria.

This article has been cited by other articles:

				F. Kubo, M. Takeichi, and S. Nakagawa Wnt2b inhibits differentiation of retinal progenitor cells in the absence of Notch activity by downregulating the expression of proneural genes Development, June 15, 2005; 132(12): 2759 - 2770. [Abstract] [Full Text] [PDF]

				M. A. I. Abou El Hassan, I. van der Meulen-Muileman, S. Abbas, and F. A. E. Kruyt Conditionally Replicating Adenoviruses Kill Tumor Cells via a Basic Apoptotic Machinery-Independent Mechanism That Resembles Necrosis-Like Programmed Cell Death J. Virol., November 15, 2004; 78(22): 12243 - 12251. [Abstract] [Full Text] [PDF]

				T. Kaufmann, S. Schlipf, J. Sanz, K. Neubert, R. Stein, and C. Borner Characterization of the signal that directs Bcl-xL, but not Bcl-2, to the mitochondrial outer membrane J. Cell Biol., January 2, 2003; 160(1): 53 - 64. [Abstract] [Full Text] [PDF]

				N. Sato, K. Matsuda, C. Sakuma, D. N. Foster, R. W. Oppenheim, and H. Yaginuma Regulated Gene Expression in the Chicken Embryo by Using Replication-Competent Retroviral Vectors J. Virol., February 15, 2002; 76(4): 1980 - 1985. [Abstract] [Full Text] [PDF]

				J. B. DuHadaway, D. Sakamuro, D. L. Ewert, and G. C. Prendergast Bin1 Mediates Apoptosis by c-Myc in Transformed Primary Cells Cancer Res., April 1, 2001; 61(7): 3151 - 3156. [Abstract] [Full Text]

				J. Sanz-Ezquerro and C Tickle Autoregulation of Shh expression and Shh induction of cell death suggest a mechanism for modulating polarising activity during chick limb development Development, January 11, 2000; 127(22): 4811 - 4823. [Abstract] [PDF]

				S. L. Holmen, D. W. Salter, W. S. Payne, J. B. Dodgson, S. H. Hughes, and M. J. Federspiel Soluble Forms of the Subgroup A Avian Leukosis Virus [ALV(A)] Receptor Tva Significantly Inhibit ALV(A) Infection In Vitro and In Vivo J. Virol., December 1, 1999; 73(12): 10051 - 10060. [Abstract] [Full Text]

				D. Fekete, S. Homburger, M. Waring, A. Riedl, and L. Garcia Involvement of programmed cell death in morphogenesis of the vertebrate inner ear Development, January 6, 1997; 124(12): 2451 - 2461. [Abstract] [PDF]