Cell Growth & Differentiation, Vol 5, Issue 2 231-237, Copyright © 1994 by American Association of Cancer Research

ARTICLES

Retinoblastoma antioncogene is involved in the inhibition of myogenesis by polyomavirus large T antigen

R Maione, GM Fimia, P Holman, B Schaffhausen and P Amati
Dipartimento di Biopatologia Umana, Universita di Roma La Sapienza, Italy.

The expression of polyomavirus large T antigen in stably transfected C2 myoblast cells inhibits terminal differentiation without inducing a transformed phenotype. In the present work, we report on the lifting of this inhibition by a mutation that prevents polyomavirus large T antigen from binding to the product of the retinoblastoma susceptibility gene (p105 RB). In contrast with cells containing wild-type large T, those with the Rb binding site mutant large T showed the same up-regulation of myosine heavy chain and myogenin mRNA expression as control cells. Furthermore, we correlate the cell cycle alteration induced by polyomavirus large T antigen expression with the inability of the cells to undergo terminal differentiation.

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