CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grumont, R. J.
Right arrow Articles by Gerondakis, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grumont, R. J.
Right arrow Articles by Gerondakis, S.

Cell Growth & Differentiation, Vol 5, Issue 12 1321-1331, Copyright © 1994 by American Association of Cancer Research


ARTICLES

The subunit composition of NF-kappa B complexes changes during B-cell development

RJ Grumont and S Gerondakis
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.

The transcription factor NF-kappa B, shown to be essential for expression of the immunoglobulin C kappa gene, is a key regulatory component in pre-B to B-cell differentiation. While previous studies have used lymphoid cell line models, here we examine the expression and subunit composition of rel/NF-kappa B complexes in normal murine pre-B and B lymphocytes. Two major NF-kappa B complexes are detected in pre-B and B cells. A high mobility complex, found in pre-B (Cb) and B cells (C beta) is a homodimer of the NF-kappa B subunit p50. In pre-B cells, the slower migrating complex (Ca), which is predominantly cytoplasmic, is largely comprised of p50 and p65, whereas in B cells, a nuclear and cytoplasmic complex (C alpha) of identical mobility to Ca mainly consists of p50 and p75c-rel. While p50 and p65 levels do not change during pre-B to B-cell differentiation, p75c-rel is 5- to 6-fold more abundant in B cells compared to pre-B cells, a finding consistent with the switch in NF-kappa B subunit usage. During lipopolysaccharide-induced B-cell proliferation, transient up-regulation of both the nuclear p50 homodimer and p75c-rel containing complex is mirrored by a concurrent increase in c-rel and p105 but not p65 mRNA expression, a finding consistent with rel-NF-kappa B expression in B cells being controlled by an autoregulatory mechanism.


This article has been cited by other articles:


Home page
JEMHome page
E. de Valle, G. Grigoriadis, L. A. OReilly, S. N. Willis, M. J. Maxwell, L. M. Corcoran, E. Tsantikos, J. K. S. Cornish, K. A. Fairfax, A. Vasanthakumar, et al.
NF{kappa}B1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells
J. Exp. Med., April 4, 2016; 213(4): 621 - 641.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
B. N. Alves, R. Tsui, J. Almaden, M. N. Shokhirev, J. Davis-Turak, J. Fujimoto, H. Birnbaum, J. Ponomarenko, and A. Hoffmann
I{kappa}B{varepsilon} Is a Key Regulator of B Cell Expansion by Providing Negative Feedback on cRel and RelA in a Stimulus-Specific Manner
J. Immunol., April 1, 2014; 192(7): 3121 - 3132.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
I. K. Campbell, A. van Nieuwenhuijze, E. Segura, K. O'Donnell, E. Coghill, M. Hommel, S. Gerondakis, J. A. Villadangos, and I. P. Wicks
Differentiation of Inflammatory Dendritic Cells Is Mediated by NF-{kappa}B1-Dependent GM-CSF Production in CD4 T Cells
J. Immunol., May 1, 2011; 186(9): 5468 - 5477.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. A. Mihalcik, P. M. Huddleston III, X. Wu, and D. F. Jelinek
The Structure of the TNFRSF13C Promoter Enables Differential Expression of BAFF-R during B Cell Ontogeny and Terminal Differentiation
J. Immunol., July 15, 2010; 185(2): 1045 - 1054.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
I. Isomura, S. Palmer, R. J. Grumont, K. Bunting, G. Hoyne, N. Wilkinson, A. Banerjee, A. Proietto, R. Gugasyan, L. Wu, et al.
c-Rel is required for the development of thymic Foxp3+ CD4 regulatory T cells
J. Exp. Med., December 21, 2009; 206(13): 3001 - 3014.
[Abstract] [Full Text] [PDF]


Home page
Cold Spring Harb. Perspect. Biol.Home page
A. Oeckinghaus and S. Ghosh
The NF-{kappa}B Family of Transcription Factors and Its Regulation
Cold Spring Harb Perspect Biol, October 1, 2009; 1(4): a000034 - a000034.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
G. R. Anderson, R. Lujan, and K. A. Martemyanov
Changes in Striatal Signaling Induce Remodeling of RGS Complexes Containing G{beta}5 and R7BP Subunits
Mol. Cell. Biol., June 1, 2009; 29(11): 3033 - 3044.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
A. Banerjee, R. Grumont, R. Gugasyan, C. White, A. Strasser, and S. Gerondakis
NF-{kappa}B1 and c-Rel cooperate to promote the survival of TLR4-activated B cells by neutralizing Bim via distinct mechanisms
Blood, December 15, 2008; 112(13): 5063 - 5073.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
Y. Zhan, S. Gerondakis, E. Coghill, D. Bourges, Y. Xu, J. L. Brady, and A. M. Lew
Glucocorticoid-Induced TNF Receptor Expression by T Cells Is Reciprocally Regulated by NF-{kappa}B and NFAT
J. Immunol., October 15, 2008; 181(8): 5405 - 5413.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
M. O'Keeffe, R. J. Grumont, H. Hochrein, M. Fuchsberger, R. Gugasyan, D. Vremec, K. Shortman, and S. Gerondakis
Distinct roles for the NF-{kappa}B1 and c-Rel transcription factors in the differentiation and survival of plasmacytoid and conventional dendritic cells activated by TLR-9 signals
Blood, November 15, 2005; 106(10): 3457 - 3464.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
E. Vigorito, L. Gambardella, F. Colucci, S. McAdam, and M. Turner
Vav proteins regulate peripheral B-cell survival
Blood, October 1, 2005; 106(7): 2391 - 2398.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
E. Hertlein, J. Wang, K. J. Ladner, N. Bakkar, and D. C. Guttridge
RelA/p65 Regulation of I{kappa}B{beta}
Mol. Cell. Biol., June 15, 2005; 25(12): 4956 - 4968.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. Doerre, K. P. Mesires, K. M. Daley, T. McCarty, S. Knoetig, and R. B. Corley
Reductions in I{kappa}B{varepsilon} and Changes in NF-{kappa}B Activity during B Lymphocyte Differentiation
J. Immunol., January 15, 2005; 174(2): 983 - 991.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
S. O'Connor, S. D. Shumway, I. J. Amanna, C. E. Hayes, and S. Miyamoto
Regulation of Constitutive p50/c-Rel Activity via Proteasome Inhibitor-Resistant I{kappa}B{alpha} Degradation in B Cells
Mol. Cell. Biol., June 1, 2004; 24(11): 4895 - 4908.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
B. Baeza-Raja and P. Munoz-Canoves
p38 MAPK-induced Nuclear Factor-{kappa}B Activity Is Required for Skeletal Muscle Differentiation: Role of Interleukin-6
Mol. Biol. Cell, April 1, 2004; 15(4): 2013 - 2026.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
Y. Nakamura, R. J. Grumont, and S. Gerondakis
NF-{kappa}B1 Can Inhibit v-Abl-Induced Lymphoid Transformation by Functioning as a Negative Regulator of Cyclin D1 Expression
Mol. Cell. Biol., August 1, 2002; 22(15): 5563 - 5574.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. Pohl, R. Gugasyan, R. J. Grumont, A. Strasser, D. Metcalf, D. Tarlinton, W. Sha, D. Baltimore, and S. Gerondakis
The combined absence of NF-{kappa}B1 and c-Rel reveals that overlapping roles for these transcription factors in the B cell lineage are restricted to the activation and function of mature cells
PNAS, April 2, 2002; 99(7): 4514 - 4519.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
S. M. Fennewald, J. F. Aronson, L. Zhang, and N. K. Herzog
Alterations in NF-{kappa}B and RBP-J{kappa} by Arenavirus Infection of Macrophages In Vitro and In Vivo
J. Virol., February 1, 2002; 76(3): 1154 - 1162.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. Grumont, H. Hochrein, M. O'Keeffe, R. Gugasyan, C. White, I. Caminschi, W. Cook, and S. Gerondakis
C-Rel Regulates Interleukin 12 P70 Expression in Cd8+ Dendritic Cells by Specifically Inducing p35 Gene Transcription
J. Exp. Med., October 15, 2001; 194(8): 1021 - 1032.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
W. F. Tam, W. Wang, and R. Sen
Cell-Specific Association and Shuttling of I{kappa}B{alpha} Provides a Mechanism for Nuclear NF-{kappa}B in B Lymphocytes
Mol. Cell. Biol., July 15, 2001; 21(14): 4837 - 4846.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
T. Kaisho, K. Takeda, T. Tsujimura, T. Kawai, F. Nomura, N. Terada, and S. Akira
I{kappa}b Kinase {alpha} Is Essential for Mature B Cell Development and Function
J. Exp. Med., February 19, 2001; 193(4): 417 - 426.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
E. R. Fields, B. J. Seufzer, E. M. Oltz, and S. Miyamoto
A Switch in Distinct I{kappa}B{alpha} Degradation Mechanisms Mediates Constitutive NF-{kappa}B Activation in Mature B Cells
J. Immunol., May 1, 2000; 164(9): 4762 - 4767.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. J. Grumont and S. Gerondakis
Rel Induces Interferon Regulatory Factor 4 (IRF-4) Expression in Lymphocytes: Modulation of Interferon-Regulated Gene Expression by Rel/Nuclear Factor {kappa}B
J. Exp. Med., April 17, 2000; 191(8): 1281 - 1292.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
S. Gupta, M. Jiang, A. Anthony, and A. B. Pernis
Lineage-Specific Modulation of Interleukin 4 Signaling by Interferon Regulatory Factor 4
J. Exp. Med., December 20, 1999; 190(12): 1837 - 1848.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
D. C. Guttridge, C. Albanese, J. Y. Reuther, R. G. Pestell, and A. S. Baldwin Jr.
NF-{kappa}B Controls Cell Growth and Differentiation through Transcriptional Regulation of Cyclin D1
Mol. Cell. Biol., August 1, 1999; 19(8): 5785 - 5799.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
R. J. Grumont, I. J. Rourke, and S. Gerondakis
Rel-dependent induction of A1 transcription is required to protect B cells from antigen receptor ligation-induced apoptosis
Genes & Dev., February 15, 1999; 13(4): 400 - 411.
[Abstract] [Full Text]


Home page
J. Immunol.Home page
S. Gupta, D. Xia, M. Jiang, S. Lee, and A. B. Pernis
Signaling Pathways Mediated by the TNF- and Cytokine-Receptor Families Target a Common cis-Element of the IFN Regulatory Factor 1 Promoter
J. Immunol., December 1, 1998; 161(11): 5997 - 6004.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. J. Grumont, I. J. Rourke, L. A. O'Reilly, A. Strasser, K. Miyake, W. Sha, and S. Gerondakis
B Lymphocytes Differentially Use the Rel and Nuclear Factor {kappa}B1 (NF-{kappa}B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
J. Exp. Med., March 2, 1998; 187(5): 663 - 674.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
J. H. Caamano, C. A. Rizzo, S. K. Durham, D. S. Barton, C. Raventos-Suarez, C. M. Snapper, and R. Bravo
Nuclear Factor (NF)-{kappa}B2 (p100/p52) Is Required for Normal Splenic Microarchitecture and B Cell-mediated Immune Responses
J. Exp. Med., January 19, 1998; 187(2): 185 - 196.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
W. C. Sha
Regulation of Immune Responses by NF-{kappa}B/Rel Transcription Factors
J. Exp. Med., January 19, 1998; 187(2): 143 - 146.
[Full Text] [PDF]


Home page
Genes Dev.Home page
I M Verma, J K Stevenson, E M Schwarz, D Van Antwerp, and S Miyamoto
Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.
Genes & Dev., November 15, 1995; 9(22): 2723 - 2735.
[PDF]


Home page
Genes Dev.Home page
F Kontgen, R J Grumont, A Strasser, D Metcalf, R Li, D Tarlinton, and S Gerondakis
Mice lacking the c-rel proto-oncogene exhibit defects in lymphocyte proliferation, humoral immunity, and interleukin-2 expression.
Genes & Dev., August 15, 1995; 9(16): 1965 - 1977.
[Abstract] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1994 by the American Association of Cancer Research.