Cell Growth & Differentiation, Vol 5, Issue 10 1051-1059, Copyright © 1994 by American Association of Cancer Research

ARTICLES

Release from G0/G1 arrest induced by dimethyl sulfoxide in human lymphoid cells: regulation of synthesis and activation of the p33cdk2 and p34cdc2 kinases

K Takase, N Terada, A Szepesi, H Teraoka, EW Gelfand and JJ Lucas
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.

Raji cells, a human Burkitt's lymphoma-derived cell line, will accumulate in a G0-like state upon prolonged (5-6 days) incubation in medium containing 1.5% dimethyl sulfoxide (DMSO). After removal of DMSO, the cells reenter the cell cycle in a synchronous manner and proliferate. After 5.5 days incubation in DMSO, S phase entry occurs at about 21-24 h after release, which is about the length of the first G1 phase of normal human lymphocytes which are stimulated in vitro to enter the cell cycle. The G0-like state of arrested cells and the sequence of events occurring after release from DMSO mimic, in most ways studied, those of normal lymphocytes. Arrested Raji cells lack many cell cycle-regulated molecules, including cyclin A, proliferating cell nuclear antigen, and the p34cdc2 kinase. They contain only hypophosphorylated p110Rb and a low level of enzymatically inactive p33cdk2 kinase. After reentering the cell cycle, a series of events occurred, including phosphorylation of p110Rb and accumulation of the cyclin A and proliferating cell nuclear antigen proteins in mid-G1 and the accumulation of the p33cdk2 and p34cdc2 proteins beginning in late G1, just prior to S-phase entry. Cyclin E levels in Raji cells appeared to be less regulated than in normal cells, with high levels of this protein being present in resting cells and throughout the entire cell cycle. The time courses of activation of the p34cdc2 and p33cdk2 kinases were similar; both became detectable at about 21 h after release and increased greatly in early S.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. Kakinuma, Y. Tanaka, R. Chinzei, M. Watanabe, K. Shimizu-saito, Y. Hara, K. Teramoto, S. Arii, C. Sato, K. Takase, et al. Human Umbilical Cord Blood as a Source of Transplantable Hepatic Progenitor Cells Stem Cells, March 1, 2003; 21(2): 217 - 227. [Abstract] [Full Text] [PDF]

				M. Fiore, R. Zanier, and F. Degrassi Reversible G1 arrest by dimethyl sulfoxide as a new method to synchronize Chinese hamster cells Mutagenesis, September 1, 2002; 17(5): 419 - 424. [Abstract] [Full Text] [PDF]

				P. Liakos, N. Bourmeyster, G. Defaye, E. M. Chambaz, and S. P. Bottari ANG II AT1 and AT2 receptors both inhibit bFGF-induced proliferation of bovine adrenocortical cells Am J Physiol Cell Physiol, October 1, 1997; 273(4): C1324 - C1334. [Abstract] [Full Text] [PDF]