CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Domann, F. E.
Right arrow Articles by Bowden, G. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Domann, F. E.
Right arrow Articles by Bowden, G. T.

Cell Growth & Differentiation, Vol 5, Issue 1 9-16, Copyright © 1994 by American Association of Cancer Research


ARTICLES

Stable expression of a c-JUN deletion mutant in two malignant mouse epidermal cell lines blocks tumor formation in nude mice

FE Domann, JP Levy, MJ Birrer and GT Bowden
Department of Radiation Oncology, University of Arizona, Tucson 85724.

We have stably expressed a trans-activation suppressing deletion mutant of the human c-jun gene (TAM-67) in the malignant mouse epidermal cell lines 10Gy5 and PDV. Expression of the p26 mJUN protein blocked both constitutive and inducible transcriptional trans-activation of several AP-1 responsive reporter chloramphenicol acetyltransferase constructs. p26 mJUN was able to block both 12-O-tetradecanoylphorbol-13-acetate (TPA) and okadaic acid induced expression of the mouse stromelysin gene in 10Gy5 cells and TPA induced expression of the urokinase-type plasminogen activator gene in PDV cells as determined by Northern analyses. Both genes contain TPA response elements in their promoter regions and are known to be AP-1 responsive. The presence of p26 mJUN in nuclear extracts, as determined by Western blotting, did not detectably alter the DNA binding activity of endogenous AP-1 as determined by gel shift analysis with an oligonucleotide containing a single high affinity AP-1 binding site. UV cross-linking studies coupled with Western analyses identified DNA bound cJUN but not mJUN in nuclear extracts of stably transfected cell lines, suggesting that the mutant JUN protein may exert some of its antioncogenic effects in malignant mouse epidermal cells by a mechanism(s) not involving DNA binding. Malignant mouse epidermal cells which stably expressed the mutant JUN protein were not only inhibited in their AP-1 trans-activation response, but also in their ability to form s.c. tumors in nude mice. These results indicate that inhibition of AP-1 mediated transcriptional trans-activation alone can be sufficient to suppress the tumorigenic phenotype in a subset of malignant mouse epidermal cells.


This article has been cited by other articles:


Home page
Mol Cancer ResHome page
S. Ghose, N. V. Oleinik, N. I. Krupenko, and S. A. Krupenko
10-Formyltetrahydrofolate Dehydrogenase-Induced c-Jun-NH2-Kinase Pathways Diverge at the c-Jun-NH2-Kinase Substrate Level in Cells with Different p53 Status
Mol. Cancer Res., January 1, 2009; 7(1): 99 - 107.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. Y. Zhang, A. E. Adams, T. W. Ridky, S. Tao, and P. A. Khavari
Tumor Necrosis Factor Receptor 1/c-Jun-NH2-Kinase Signaling Promotes Human Neoplasia
Cancer Res., April 15, 2007; 67(8): 3827 - 3834.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
C. P. Matthews, A. M. Birkholz, A. R. Baker, C. M. Perella, G. R. Beck Jr., M. R. Young, and N. H. Colburn
Dominant-Negative Activator Protein 1 (TAM67) Targets Cyclooxygenase-2 and Osteopontin under Conditions in which It Specifically Inhibits Tumorigenesis
Cancer Res., March 15, 2007; 67(6): 2430 - 2438.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
X. Shi-Wen, F. Rodriguez-Pascual, S. Lamas, A. Holmes, S. Howat, J. D. Pearson, M. R. Dashwood, R. M. du Bois, C. P. Denton, C. M. Black, et al.
Constitutive ALK5-Independent c-Jun N-Terminal Kinase Activation Contributes to Endothelin-1 Overexpression in Pulmonary Fibrosis: Evidence of an Autocrine Endothelin Loop Operating through the Endothelin A and B Receptors
Mol. Cell. Biol., July 15, 2006; 26(14): 5518 - 5527.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
M. A. Bachelor, S. J. Cooper, E. T. Sikorski, and G. T. Bowden
Inhibition of p38 Mitogen-Activated Protein Kinase and Phosphatidylinositol 3-Kinase Decreases UVB-Induced Activator Protein-1 and Cyclooxygenase-2 in a SKH-1 Hairless Mouse Model
Mol. Cancer Res., February 1, 2005; 3(2): 90 - 99.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
P. Papassava, V. G. Gorgoulis, D. Papaevangeliou, S. Vlahopoulos, H. van Dam, and V. Zoumpourlis
Overexpression of Activating Transcription Factor-2 Is Required for Tumor Growth and Progression in Mouse Skin Tumors
Cancer Res., December 1, 2004; 64(23): 8573 - 8584.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
M. Kielosto, P. Nummela, R. Katainen, V. Leaner, M. J. Birrer, and E. Holtta
Reversible Regulation of the Transformed Phenotype of Ornithine Decarboxylase- and Ras-Overexpressing Cells by Dominant-Negative Mutants of c-Jun
Cancer Res., June 1, 2004; 64(11): 3772 - 3779.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
S. J. Cooper, J. MacGowan, J. Ranger-Moore, M. R. Young, N. H. Colburn, and G. T. Bowden
Expression of Dominant Negative c-jun Inhibits Ultraviolet B-Induced Squamous Cell Carcinoma Number and Size in an SKH-1 Hairless Mouse Model
Mol. Cancer Res., September 1, 2003; 1(11): 848 - 854.
[Abstract] [Full Text] [PDF]


Home page
Cancer Epidemiol. Biomarkers Prev.Home page
W. B. Bair III, N. Hart, J. Einspahr, G. Liu, Z. Dong, D. Alberts, and G. T. Bowden
Inhibitory Effects of Sodium Salicylate and Acetylsalicylic Acid on UVB-induced Mouse Skin Carcinogenesis
Cancer Epidemiol. Biomarkers Prev., December 1, 2002; 11(12): 1645 - 1652.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
E. J. Thompson, J. MacGowan, M. R. Young, N. Colburn, and G. T. Bowden
A Dominant Negative c-jun Specifically Blocks Okadaic Acid-induced Skin Tumor Promotion
Cancer Res., June 1, 2002; 62(11): 3044 - 3047.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
Q.-B. She, N. Chen, A. M. Bode, R. A. Flavell, and Z. Dong
Deficiency of c-Jun-NH2-terminal Kinase-1 in Mice Enhances Skin Tumor Development by 12-O-Tetradecanoylphorbol-13-Acetate
Cancer Res., March 1, 2002; 62(5): 1343 - 1348.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
M. R. Young, R. Nair, N. Bucheimer, P. Tulsian, N. Brown, C. Chapp, T.-C. Hsu, and N. H. Colburn
Transactivation of Fra-1 and Consequent Activation of AP-1 Occur Extracellular Signal-Regulated Kinase Dependently
Mol. Cell. Biol., January 15, 2002; 22(2): 587 - 598.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
N. Chen, W.-Y. Ma, Q.-B. She, E. Wu, G. Liu, A. M. Bode, and Z. Dong
Transactivation of the Epidermal Growth Factor Receptor Is Involved in 12-O-Tetradecanoylphorbol-13-acetate-induced Signal Transduction
J. Biol. Chem., December 14, 2001; 276(50): 46722 - 46728.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
G. Liu, A. Bode, W.-Y. Ma, S. Sang, C.-T. Ho, and Z. Dong
Two Novel Glycosides from the Fruits of Morinda Citrifolia (Noni) Inhibit AP-1 Transactivation and Cell Transformation in the Mouse Epidermal JB6 Cell Line
Cancer Res., August 1, 2001; 61(15): 5749 - 5756.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
G. Liu, N. Chen, A. Kaji, A. M. Bode, C. A. Ryan, and Z. Dong
Proteinase inhibitors I and II from potatoes block UVB-induced AP-1 activity by regulating the AP-1 protein compositional patterns in JB6 cells
PNAS, May 8, 2001; 98(10): 5786 - 5791.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
S. Zhong, J. A. Quealy, A. M. Bode, M. Nomura, A. Kaji, W.-Y. Ma, and Z. Dong
Organ-specific Activation of Activator Protien-1 in Transgenic Mice by 12-O-Tetradecanoylphorbol-13-acetate with Different Administration Methods
Cancer Res., May 1, 2001; 61(10): 4084 - 4091.
[Abstract] [Full Text]


Home page
Cancer Res.Home page
T.-C. Hsu, R. Nair, P. Tulsian, C. E. Camalier, G. A. Hegamyer, M. R. Young, and N. H. Colburn
Transformation Nonresponsive Cells Owe Their Resistance to Lack of p65/Nuclear Factor-{{kappa}}B Activation
Cancer Res., May 1, 2001; 61(10): 4160 - 4168.
[Abstract] [Full Text]


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. P. Zhong, W.-Y. Ma, J. A. Quealy, Y. Zhang, and Z. Dong
Organ-specific distribution of AP-1 in AP-1 luciferase transgenic mice during the maturation process
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2001; 280(2): R376 - R381.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
S. E. Rutberg, T. L. Adams, A. Glick, M. T. Bonovich, C. Vinson, and S. H. Yuspa
Activator Protein 1 Transcription Factors Are Fundamental to v-rasHa-induced Changes in Gene Expression in Neoplastic Keratinocytes
Cancer Res., November 1, 2000; 60(22): 6332 - 6338.
[Abstract] [Full Text]


Home page
Cancer Res.Home page
Y.-P. Lu, Y.-R. Lou, X. H. Li, J. G. Xie, D. Brash, M.-T. Huang, and A. H. Conney
Stimulatory Effect of Oral Administration of Green Tea or Caffeine on Ultraviolet Light-induced Increases in Epidermal Wild-Type p53, p21(WAF1/CIP1), and Apoptotic Sunburn Cells in SKH-1 Mice
Cancer Res., September 1, 2000; 60(17): 4785 - 4791.
[Abstract] [Full Text]


Home page
Cancer Res.Home page
J. Y. Chung, C. Huang, X. Meng, Z. Dong, and C. S. Yang
Inhibition of Activator Protein 1 Activity and Cell Growth by Purified Green Tea and Black Tea Polyphenols in H-ras-transformed Cells: Structure-Activity Relationship and Mechanisms Involved
Cancer Res., September 1, 1999; 59(18): 4610 - 4617.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. R. Young, J.-J. Li, M. Rincon, R. A. Flavell, B. K. Sathyanarayana, R. Hunziker, and N. Colburn
Transgenic mice demonstrate AP-1 (activator protein-1) transactivation is required for tumor promotion
PNAS, August 17, 1999; 96(17): 9827 - 9832.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
C. Huang, W.-Y. Ma, J. Li, A. Goranson, and Z. Dong
Requirement of Erk, but Not JNK, for Arsenite-induced Cell Transformation
J. Biol. Chem., May 21, 1999; 274(21): 14595 - 14601.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. F. Rosenberger, J. S. Finch, A. Gupta, and G. T. Bowden
Extracellular Signal-regulated Kinase 1/2-mediated Phosphorylation of JunD and FosB Is Required for Okadaic Acid-induced Activator Protein 1 Activation
J. Biol. Chem., January 8, 1999; 274(2): 1124 - 1130.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
W. Chen, A. H. Borchers, Z. Dong, M. B. Powell, and G. T. Bowden
UVB Irradiation-induced Activator Protein-1 Activation Correlates with Increased c-fos Gene Expression in a Human Keratinocyte Cell Line
J. Biol. Chem., November 27, 1998; 273(48): 32176 - 32181.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
A. Malliri, M. Symons, R. F. Hennigan, A. F.L. Hurlstone, R. F. Lamb, T. Wheeler, and B. W. Ozanne
The Transcription Factor AP-1 Is Required for EGF-induced Activation of Rho-like GTPases, Cytoskeletal Rearrangements, Motility, and In Vitro Invasion of A431 Cells
J. Cell Biol., November 16, 1998; 143(4): 1087 - 1099.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
S. M. Jacobs-Helber, A. Wickrema, M. J. Birrer, and S. T. Sawyer
AP1 Regulation of Proliferation and Initiation of Apoptosis in Erythropoietin-Dependent Erythroid Cells
Mol. Cell. Biol., July 1, 1998; 18(7): 3699 - 3707.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. Huang, W.-Y. Ma, M. R. Young, N. Colburn, and Z. Dong
Shortage of mitogen-activated protein kinase is responsible for resistance to AP-1 transactivation and transformation in mouse JB6 cells
PNAS, January 6, 1998; 95(1): 156 - 161.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
C. Huang, W.-Y. Ma, D. Hanenberger, M. P. Cleary, G. T. Bowden, and Z. Dong
Inhibition of Ultraviolet B-induced Activator Protein-1 (AP-1) Activity by Aspirin in AP-1-Luciferase Transgenic Mice
J. Biol. Chem., October 17, 1997; 272(42): 26325 - 26331.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. D. Klein, A. H. Borchers, P. Sundareshan, C. Bougelet, M. R. Berkman, R. B. Nagle, and G. T. Bowden
Interleukin-1{beta} Secreted from Monocytic Cells Induces the Expression of Matrilysin in the Prostatic Cell Line LNCaP
J. Biol. Chem., May 30, 1997; 272(22): 14188 - 14192.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. Huang, W.-Y. Ma, M. I. Dawson, M. Rincon, R. A. Flavell, and Z. Dong
Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid
PNAS, May 27, 1997; 94(11): 5826 - 5830.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
C. Huang, P. C. Schmid, W.-Y. Ma, H. H. O. Schmid, and Z. Dong
Phosphatidylinositol-3 Kinase Is Necessary for 12-O-Tetradecanoylphorbol-13-acetate-induced Cell Transformation and Activated Protein 1 Activation
J. Biol. Chem., February 14, 1997; 272(7): 4187 - 4194.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
Z. Dong, R.-H. Xu, J. Kim, S.-N. Zhan, W.-Y. Ma, N. H. Colburn, and H.-f. Kung
AP-1/Jun Is Required for Early Xenopus Development and Mediates Mesoderm Induction by Fibroblast Growth Factor but Not by Activin
J. Biol. Chem., April 26, 1996; 271(17): 9942 - 9946.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
E. Lengyel, E. Stepp, R. Gum, and D. Boyd
Involvement of a Mitogen-activated Protein Kinase Signaling Pathway in the Regulation of Urokinase Promoter Activity by c-Ha-ras
J. Biol. Chem., September 29, 1995; 270(39): 23007 - 23012.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
G. Liu, N. Chen, A. Kaji, A. M. Bode, C. A. Ryan, and Z. Dong
Proteinase inhibitors I and II from potatoes block UVB-induced AP-1 activity by regulating the AP-1 protein compositional patterns in JB6 cells
PNAS, May 8, 2001; 98(10): 5786 - 5791.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1994 by the American Association of Cancer Research.