CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grumont, R. J.
Right arrow Articles by Gerondakis, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grumont, R. J.
Right arrow Articles by Gerondakis, S.

Cell Growth & Differentiation, Vol 4, Issue 9 731-743, Copyright © 1993 by American Association of Cancer Research


ARTICLES

rel/NF-kappa B nuclear complexes that bind kB sites in the murine c-rel promoter are required for constitutive c-rel transcription in B-cells

RJ Grumont, IB Richardson, C Gaff and S Gerondakis
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.

The c-rel protooncogene, a member of a transcription factor family that includes NF-kappa B, displays a complex pattern of gene expression. To understand the basis of this expression, the regulatory region upstream of the murine c-rel transcription start sites has been cloned and characterized. Transcription of the murine c-rel gene initiates at multiple sites downstream of a GC-rich region conserved in the chicken c-rel promoter. This conserved region contains consensus transcription factor binding sites for SP-1 and NF-kappa B (kB3 site) and is sufficient for basal expression in Jurkat T-cells. In contrast, two additional NF-kappa B-like sites (kB1 and kB2) and an octamer consensus binding site, all located upstream of the conserved region, are required for expression of promoter-reporter gene constructs in the B-cell line I29B. NF-kappa B sites kB1 and kB3 bind p50/65 and p50 homodimers, whereas kB2 binds a distinct complex. The consensus octamer site, although only able to bind Oct1 and Oct2 with low affinity, appears to overlap with a binding site for a novel protein(s) expressed in I29B cells. Cotransfection studies show that p75-c-rel and a carboxyl-terminal truncated c-rel protein that lacks the known trans-activating domain both up-regulate the c-rel promoter in I29B cells via a mechanism independent of the NF-kappa B motifs, whereas a mutant c-rel protein lacking the DNA binding domain has no effect. Together, these findings suggest that, in this B-cell line, trans-activation of the c-rel promoter by rel proteins is via an indirect mechanism.


This article has been cited by other articles:


Home page
J Biol ChemHome page
A. Asatryan and N. G. Bazan
Molecular mechanisms of signaling via the docosanoid neuroprotectin D1 for cellular homeostasis and neuroprotection
J. Biol. Chem., July 28, 2017; 292(30): 12390 - 12397.
[Abstract] [Full Text] [PDF]


Home page
J. Leukoc. Biol.Home page
T. T. Chang, I. Walther, C.-F. Li, J. Boonyaratanakornkit, G. Galleri, M. A. Meloni, P. Pippia, A. Cogoli, and M. Hughes-Fulford
The Rel/NF-{kappa}B pathway and transcription of immediate early genes in T cell activation are inhibited by microgravity
J. Leukoc. Biol., December 1, 2012; 92(6): 1133 - 1145.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
W. N. Khan
B Cell Receptor and BAFF Receptor Signaling Regulation of B Cell Homeostasis
J. Immunol., September 15, 2009; 183(6): 3561 - 3567.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
J. C. Nolz, M. E. Fernandez-Zapico, and D. D. Billadeau
TCR/CD28-Stimulated Actin Dynamics Are Required for NFAT1-Mediated Transcription of c-rel Leading to CD28 Response Element Activation
J. Immunol., July 15, 2007; 179(2): 1104 - 1112.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
M. W. Hansberger, J. A. Campbell, P. Danthi, P. Arrate, K. N. Pennington, K. B. Marcu, D. W. Ballard, and T. S. Dermody
I{kappa}B Kinase Subunits {alpha} and {gamma} Are Required for Activation of NF-{kappa}B and Induction of Apoptosis by Mammalian Reovirus
J. Virol., February 1, 2007; 81(3): 1360 - 1371.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
P.-Y. Chang and S. Miyamoto
Nuclear Factor-{kappa}B Dimer Exchange Promotes a p21waf1/cip1 Superinduction Response in Human T Leukemic Cells
Mol. Cancer Res., February 1, 2006; 4(2): 101 - 112.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
E. Vigorito, L. Gambardella, F. Colucci, S. McAdam, and M. Turner
Vav proteins regulate peripheral B-cell survival
Blood, October 1, 2005; 106(7): 2391 - 2398.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. Doerre, K. P. Mesires, K. M. Daley, T. McCarty, S. Knoetig, and R. B. Corley
Reductions in I{kappa}B{varepsilon} and Changes in NF-{kappa}B Activity during B Lymphocyte Differentiation
J. Immunol., January 15, 2005; 174(2): 983 - 991.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
I. J. Frew, V. E. Hammond, R. A. Dickins, J. M. W. Quinn, C. R. Walkley, N. A. Sims, R. Schnall, N. G. Della, A. J. Holloway, M. R. Digby, et al.
Generation and Analysis of Siah2 Mutant Mice
Mol. Cell. Biol., December 15, 2003; 23(24): 9150 - 9161.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
A. Hoffmann, T. H. Leung, and D. Baltimore
Genetic analysis of NF-{kappa}B/Rel transcription factors defines functional specificities
EMBO J., October 15, 2003; 22(20): 5530 - 5539.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. Grumont, H. Hochrein, M. O'Keeffe, R. Gugasyan, C. White, I. Caminschi, W. Cook, and S. Gerondakis
C-Rel Regulates Interleukin 12 P70 Expression in Cd8+ Dendritic Cells by Specifically Inducing p35 Gene Transcription
J. Exp. Med., October 15, 2001; 194(8): 1021 - 1032.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
U. D. Bajpai, K. Zhang, M. Teutsch, R. Sen, and H. H. Wortis
Bruton's Tyrosine Kinase Links the B Cell Receptor to Nuclear Factor {kappa}b Activation
J. Exp. Med., May 15, 2000; 191(10): 1735 - 1744.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
E. R. Fields, B. J. Seufzer, E. M. Oltz, and S. Miyamoto
A Switch in Distinct I{kappa}B{alpha} Degradation Mechanisms Mediates Constitutive NF-{kappa}B Activation in Mature B Cells
J. Immunol., May 1, 2000; 164(9): 4762 - 4767.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
R. J. Grumont and S. Gerondakis
Rel Induces Interferon Regulatory Factor 4 (IRF-4) Expression in Lymphocytes: Modulation of Interferon-Regulated Gene Expression by Rel/Nuclear Factor {kappa}B
J. Exp. Med., April 17, 2000; 191(8): 1281 - 1292.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
S. Doerre and R. B. Corley
Constitutive Nuclear Translocation of NF-{kappa}B in B Cells in the Absence of I{kappa}B Degradation
J. Immunol., July 1, 1999; 163(1): 269 - 277.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. M. Sheehy and M. S. Schlissel
Overexpression of RelA Causes G1 Arrest and Apoptosis in a Pro-B Cell Line
J. Biol. Chem., March 26, 1999; 274(13): 8708 - 8716.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
R. J. Grumont, I. J. Rourke, and S. Gerondakis
Rel-dependent induction of A1 transcription is required to protect B cells from antigen receptor ligation-induced apoptosis
Genes & Dev., February 15, 1999; 13(4): 400 - 411.
[Abstract] [Full Text]


Home page
Mol. Cell. Biol.Home page
S. Miyamoto, B. J. Seufzer, and S. D. Shumway
Novel I{kappa}B{alpha} Proteolytic Pathway in WEHI231 Immature B Cells
Mol. Cell. Biol., January 1, 1998; 18(1): 19 - 29.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
H. Yasui, W. Butscher, M. Cohen, N. Spriggs, R. Wersto, E. C. Kohn, L. Liotta, and K. Gardner
Selective Inhibition of Mitogen-induced Transactivation of the HIV Long Terminal Repeat by Carboxyamidotriazole: CALCIUM INFLUX BLOCKADE REPRESSES HIV-1 TRANSCRIPTIONAL ACTIVATION
J. Biol. Chem., November 7, 1997; 272(45): 28762 - 28770.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
F Kontgen, R J Grumont, A Strasser, D Metcalf, R Li, D Tarlinton, and S Gerondakis
Mice lacking the c-rel proto-oncogene exhibit defects in lymphocyte proliferation, humoral immunity, and interleukin-2 expression.
Genes & Dev., August 15, 1995; 9(16): 1965 - 1977.
[Abstract] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1993 by the American Association of Cancer Research.