CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sablitzky, F.
Right arrow Articles by Phillips, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sablitzky, F.
Right arrow Articles by Phillips, R. A.

Cell Growth & Differentiation, Vol 4, Issue 6 451-459, Copyright © 1993 by American Association of Cancer Research


ARTICLES

High frequency expression of integrated proviruses derived from enhancer trap retroviruses

F Sablitzky, JI Jonsson, BL Cohen and RA Phillips
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.

Since retroviruses integrate preferentially into transcriptionally active loci, the provirus may come under the control of regulatory elements of the gene into which it integrated and thus become a functional tag for that gene. In order to determine the frequency of retroviral integration near active endogenous enhancer elements, a retroviral enhancer trap vector was constructed. Lacking the long terminal repeat enhancer, expression of the neomycin resistance (neo) gene, used as a reporter, is dependent upon endogenous enhancer elements able to activate the long terminal repeat promoter. Infection of murine fibroblast cells indicated that a high proportion of the proviral copies expressed the neo gene. Infection of hematopoietic lines confirmed this high frequency of expression of integrated proviruses. Overall, between 43 and 74% of proviruses integrated into several different cell lines expressed the neo gene. These data suggest that retroviral integration is not only dependent upon transcriptional activity of the genomic target sites, but, more specifically, retroviruses preferentially integrate near active enhancer elements which are often associated with developmentally regulated genes.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1993 by the American Association of Cancer Research.