CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thompson, N. L.
Right arrow Articles by Hixson, D. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thompson, N. L.
Right arrow Articles by Hixson, D. C.

Cell Growth & Differentiation, Vol 4, Issue 4 257-268, Copyright © 1993 by American Association of Cancer Research


ARTICLES

Spatiotemporal expression of two cell-cell adhesion molecule 105 isoforms during liver development

NL Thompson, MA Panzica, G Hull, SH Lin, TR Curran, PA Gruppuso, O Baum, W Reutter and DC Hixson
Department of Medical Oncology, Rhode Island Hospital, Brown University, Providence 02903.

The rat liver adhesion molecule cell CAM 105 has been postulated to be involved in liver histogenesis. Recently, it was shown to exist in two isoforms that differ in the length of their cytoplasmic domains (O. Culic, Q-H. Huang, D. Flanagan, D. C. Hixson, and S. H. Lin, Biochem. J., 285: 47-53, 1992). Isoform-specific differences in phosphorylation and aggregation function have been observed. To study the possible roles of these isoforms during liver development, we have used both complementary DNA probes and isoform-specific antibodies to examine their temporal and spatial expression. Northern blot analysis revealed low steady-state levels of a 4.0-kilobase RNA at 15-20 days gestation, which increased dramatically at birth and remained at least 12-fold higher than fetal levels in neonatal and adult liver. Additional polyadenylated RNA species of 6.0 and 2.9 kilobases were detected after birth. Steady-state levels of cell CAM 105 RNA in cultured adult and fetal hepatocytes were comparable to in vivo levels, respectively, and were not influenced by treatment with transforming growth factor beta or by culture density. Increases in cell CAM 105 protein demonstrated by immunoblot analysis correlated with the RNA increases, suggesting that regulation of cell CAM 105 expression is largely transcriptional during development. Ratios of the long and short isoforms remained relatively constant after birth. Isoform-specific antipeptide antibodies localized both isoforms primarily to maturing bile canalicular domains of hepatocytes during liver development. The long isoform could not be detected in fetal liver in situ prior to 20 days, however, suggesting that specific roles may exist for these molecules during development.


This article has been cited by other articles:


Home page
Cancer Res.Home page
N. A. Laurie, M. M. Comegys, M. P. Carreiro, J. F. Brown, D. L. Flanagan, K. E. Brilliant, and D. C. Hixson
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1a-4L Suppression of Rat Hepatocellular Carcinomas
Cancer Res., December 1, 2005; 65(23): 11010 - 11017.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
I. Hunter, M. Lindh, and B. Obrink
Differential regulation of C-CAM isoforms in epithelial cells
J. Cell Sci., May 1, 1994; 107(5): 1205 - 1216.
[Abstract] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1993 by the American Association of Cancer Research.