CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Costello, R.
Right arrow Articles by Imbert, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Costello, R.
Right arrow Articles by Imbert, J.

Cell Growth & Differentiation, Vol 4, Issue 11 947-954, Copyright © 1993 by American Association of Cancer Research


ARTICLES

The role of NF-kappa B1 (p50/p105) gene expression in activation of human blood T-lymphocytes via CD2 and CD28 adhesion molecules

R Costello, C Cerdan, C Lipcey, M Algarte, Y Martin, PA Baeuerle, D Olive and J Imbert
Unite 119, Institut National de la Sante et de la Recherche Medicale, Marseille, France.

Stimulation of primary human T-lymphocytes via CD2 and CD28 adhesion molecules induces a long-lasting proliferation (> 3 weeks). This potent activation does not require accessory cells, such as monocytes, but depends on persistent interleukin 2 (IL-2) secretion and receptivity, which is associated with high and prolonged expression of the inducible CD25/IL-2 receptor alpha (IL-2R alpha) chain gene. The transcription factor NF-kappa B participates in the regulation of both IL-2 and IL-2R alpha genes, as well as multiple cellular genes involved in T-cell proliferation. To evaluate the role of NF-kappa B in human peripheral blood T-lymphocytes, we previously analyzed the activation of NF-kappa B-related complexes in response to CD2+CD28 costimulation. We demonstrated a long-term induction of p50/p65 heterodimer, a putative p65/c-Rel heterodimer, and a constitutive nuclear expression of KBF1/p50 homodimers. As the role of p50 remains unclear, we focused our present study on NF-kappa B1 (p50/p105) gene regulation. Using electrophoretic mobility shift assays and Western and Northern blot analyses, we studied NF-kappa B1 gene expression during T-cell stimulation via CD2+CD28. We observed a transient 4- to 5-fold increase of NF-kappa B1 gene expression at both the mRNA and protein levels, lasting for at least 24 h. p50 DNA-binding activity apparently stays highly controlled when p105 expression is enhanced by a physiological stimulus of peripheral blood T-cells. Partial inhibition of p50 and p105 expression by NF-kappa B1 antisense oligonucleotides significantly reduced T-cell proliferation and CD25/IL-2R alpha cell surface expression.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
J. Immunol.Home page
W. Lai, M. Yu, M.-N. Huang, F. Okoye, A. D. Keegan, and D. L. Farber
Transcriptional Control of Rapid Recall by Memory CD4 T Cells
J. Immunol., July 1, 2011; 187(1): 133 - 140.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Fan, R. D. Ye, and A. B. Malik
Transcriptional mechanisms of acute lung injury
Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1037 - L1050.
[Abstract] [Full Text] [PDF]


Home page
J. Immunol.Home page
X. Wang, H. Luo, H. Chen, W. Duguid, and J. Wu
Role of Proteasomes in T Cell Activation and Proliferation
J. Immunol., January 15, 1998; 160(2): 788 - 801.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
B. Kahn-Perles, C. Lipcey, P. Lecine, D. Olive, and J. Imbert
Temporal and Subunit-specific Modulations of the Rel/NF-{kappa}B Transcription Factors Through CD28 Costimulation
J. Biol. Chem., August 29, 1997; 272(35): 21774 - 21783.
[Abstract] [Full Text] [PDF]


Home page
JEMHome page
P. C. Cogswell, M. W. Mayo, and A. S. Baldwin Jr.
Involvement of Egr-1/RelA Synergy in Distinguishing T Cell Activation from Tumor Necrosis Factor-{alpha}-induced NF-{kappa}B1 Transcription
J. Exp. Med., February 3, 1997; 185(3): 491 - 498.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1993 by the American Association of Cancer Research.