CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Datta, R.
Right arrow Articles by Kufe, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Datta, R.
Right arrow Articles by Kufe, D.

Cell Growth & Differentiation, Vol 3, Issue 9 637-644, Copyright © 1992 by American Association of Cancer Research


ARTICLES

Down-regulation of cell cycle control genes by ionizing radiation

R Datta, R Hass, H Gunji, R Weichselbaum and D Kufe
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.

The cellular responses to ionizing radiation include growth arrest, DNA repair, and lethality. However, little is known about the signaling events responsible for these responses. The present studies have examined the effects of ionizing radiation on the expression of genes involved in cell cycle control. The results demonstrate that the treatment of asynchronous cells with 20 Gy ionizing radiation is associated with transient down-regulation of the cdc2, cyclin A, cyclin B, and cdc25 genes. This effect was associated with transient induction of the c-jun gene. RNA stability studies demonstrate that the down-regulation of gene expression following ionizing radiation exposure is at least in part due to a decrease in transcript half-life. Other studies were performed with elutriated cells enriched for populations in G1 and S phases. Treatment of G1 enriched cell populations with 10 Gy resulted in a selective decrease in cyclin B mRNA levels, whereas this effect on cyclin B expression was less pronounced at 5 Gy and undetectable at 1 Gy. Similar results were obtained with S phase enriched cells. Taken together with clonogenic survival studies, these findings indicate that down-regulation of cell cycle control gene expression is associated with lethality, whereas lower doses of ionizing radiation have little, if any, effect on the expression of these genes. The findings also suggest that DNA damage may activate signaling events which regulate expression of cell cycle control genes.


This article has been cited by other articles:


Home page
Cancer Res.Home page
L. Geng, K. C. Cuneo, A. Fu, T. Tu, P. W. Atadja, and D. E. Hallahan
Histone Deacetylase (HDAC) Inhibitor LBH589 Increases Duration of {gamma}-H2AX Foci and Confines HDAC4 to the Cytoplasm in Irradiated Non-Small Cell Lung Cancer
Cancer Res., December 1, 2006; 66(23): 11298 - 11304.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
H. Miyata, Y. Doki, H. Yamamoto, K. Kishi, H. Takemoto, Y. Fujiwara, T. Yasuda, M. Yano, M. Inoue, H. Shiozaki, et al.
Overexpression of CDC25B Overrides Radiation-induced G2-M Arrest and Results in Increased Apoptosis in Esophageal Cancer Cells
Cancer Res., April 1, 2001; 61(7): 3188 - 3193.
[Abstract] [Full Text]


Home page
EMBO J.Home page
W. Wang, M. C. Caldwell, S. Lin, H. Furneaux, and M. Gorospe
HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation
EMBO J., May 15, 2000; 19(10): 2340 - 2350.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
H. Barth, M. Klingler, K. Aktories, and V. Kinzel
Clostridium botulinum C2 Toxin Delays Entry into Mitosis and Activation of p34cdc2 Kinase and cdc25-C Phosphatase in HeLa cells
Infect. Immun., October 1, 1999; 67(10): 5083 - 5090.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
T. M. Passalaris, J. A. Benanti, L. Gewin, T. Kiyono, and D. A. Galloway
The G2 Checkpoint Is Maintained by Redundant Pathways
Mol. Cell. Biol., September 1, 1999; 19(9): 5872 - 5881.
[Abstract] [Full Text] [PDF]


Home page
Hum Exp ToxicolHome page
D. A Boothman, E. Odegaard, C.-R. Yang, K. Hosley, and M. S Mendonca
Molecular analyses of adaptive survival responses (ASRs): role of ASRs in radiotherapy
Human and Experimental Toxicology, August 1, 1998; 17(8): 448 - 453.
[Abstract] [PDF]


Home page
Genes Dev.Home page
M Pagano, A M Theodoras, S W Tam, and G F Draetta
Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts.
Genes & Dev., July 15, 1994; 8(14): 1627 - 1639.
[Abstract] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1992 by the American Association of Cancer Research.