CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jetten, A. M.
Right arrow Articles by Lotan, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jetten, A. M.
Right arrow Articles by Lotan, R.

Cell Growth & Differentiation, Vol 3, Issue 8 549-556, Copyright © 1992 by American Association of Cancer Research


ARTICLES

Expression of a preprorelaxin-like gene during squamous differentiation of rabbit tracheobronchial epithelial cells and its suppression by retinoic acid

AM Jetten, SH Bernacki, EE Floyd, NA Saunders, J Pieniazek and R Lotan
Cell Biology Section, Nationai Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina 27709.

Squamous cell differentiation in tracheobronchial epithelial cells is accompanied by many biochemical and molecular changes. One of the molecular changes in rabbit tracheal epithelial (RbTE) cells is the differential expression of a squamous cell-specific mRNA encoded by the complementary DNA SQ10. In this study, we sequenced SQ10 complementary DNA and showed that this gene encodes a preprorelaxin-like protein. The DNA sequence of the coding region of SQ10 has 68% identity with the human preprorelaxin mRNA, whereas the deduced amino acid sequence exhibits 46% identity with human preprorelaxin. An antiserum (pepIV-Ab) was raised against a synthetic 22-amino acid oligopeptide of the protein encoded by SQ10. Immunoblot analysis of cellular extracts of squamous-differentiated cells showed that this antiserum reacted with proteins of 22 and 20 kilodaltons, possibly constituting prepro- and proforms of this protein. These proteins were undetectable in undifferentiated RbTE cells. In agreement with these observations, PepIV-Ab specifically stained the cytosol of squamous-differentiated RbTE cells but failed to stain undifferentiated cells. PepIV-Ab recognized a 20 and 16 kilodalton polypeptide in medium conditioned by squamous-differentiated RbTE cells, indicating that the prorelaxin-like protein is secreted. The amino acid sequences of three peptides that were obtained after tryptic digestion of the secreted 16 kilodalton protein were identical to sequences encoded by SQ10. Retinoids which have been shown to inhibit squamous differentiation suppressed the induction of SQ10 protein as well as mRNA in a concentration-dependent manner. The concentration at which retinoic acid caused a 50% inhibition of SQ10 mRNA levels was approximately 5 nM.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
J Biol ChemHome page
A. Medvedev, N. A. Saunders, H. Matsuura, A. Chistokhina, and A. M. Jetten
Regulation of the Transglutaminase I Gene: IDENTIFICATION OF DNA ELEMENTS INVOLVED IN ITS TRANSCRIPTIONAL CONTROL IN TRACHEOBRONCHIAL EPITHELIAL CELLS
J. Biol. Chem., February 5, 1999; 274(6): 3887 - 3896.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. J. Austin, W. Fujimoto, K. W. Marvin, T. M. Vollberg, L. Lorand, and A. M. Jetten
Cloning and Regulation of Cornifin , a New Member of the Cornifin/spr Family: SUPPRESSION BY RETINOIC ACID RECEPTOR-SELECTIVE RETINOIDS
J. Biol. Chem., February 16, 1996; 271(7): 3737 - 3742.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1992 by the American Association of Cancer Research.