Cell Growth & Differentiation, Vol 3, Issue 3 165-174, Copyright © 1992 by American Association of Cancer Research

ARTICLES

mmCGM1a: a mouse carcinoembryonic antigen gene family member, generated by alternative splicing, functions as an adhesion molecule

K McCuaig, C Turbide and N Beauchemin
McGill Cancer Centre, McGill University, Montreal, Canada.

Carcinoembryonic antigen is a human tumor marker and the prototype of a large family of immunoglobulin-like proteins. We have been developing a mouse model for this large protein family and have cloned a complementary DNA (cDNA) for a mouse carcinoembryonic antigen gene family member (mmCGM1a). Two transcripts expressed in several different adult mouse tissues hybridize to this cDNA, a 1.8-kilobase and a 4.6-kilobase mRNA. Sequences of many related cDNA clones indicate that they are most likely encoded by a single gene which undergoes alternative splicing. The protein encoded by the mmCGM1a cDNA shares 69% of the amino acid residues in the NH2-terminal domain with a rat liver ecto-ATPase and with the human biliary glycoprotein. Mouse fibroblast transfectant cells expressing the mmCGM1a protein on their cell surface exhibit calcium- and temperature-independent adhesion in vitro which can be specifically inhibited by an antibody raised against a carcinoembryonic antigen-related 120 kilodalton protein.

This article has been cited by other articles:

				K. J. Dery, S. Gaur, M. Gencheva, Y. Yen, J. E. Shively, and R. K. Gaur Mechanistic Control of Carcinoembryonic Antigen-related Cell Adhesion Molecule-1 (CEACAM1) Splice Isoforms by the Heterogeneous Nuclear Ribonuclear Proteins hnRNP L, hnRNP A1, and hnRNP M J. Biol. Chem., May 6, 2011; 286(18): 16039 - 16051. [Abstract] [Full Text] [PDF]

				S. J. Bender, J. M. Phillips, E. P. Scott, and S. R. Weiss Murine Coronavirus Receptors Are Differentially Expressed in the Central Nervous System and Play Virus Strain-Dependent Roles in Neuronal Spread J. Virol., November 1, 2010; 84(21): 11030 - 11044. [Abstract] [Full Text] [PDF]

				A. Hirai, N. Ohtsuka, T. Ikeda, R. Taniguchi, D. Blau, K. Nakagaki, H. S. Miura, Y. Ami, Y. K. Yamada, S. Itohara, et al. Role of Mouse Hepatitis Virus (MHV) Receptor Murine CEACAM1 in the Resistance of Mice to MHV Infection: Studies of Mice with Chimeric mCEACAM1a and mCEACAM1b J. Virol., July 1, 2010; 84(13): 6654 - 6666. [Abstract] [Full Text] [PDF]

				E. Hemmila, C. Turbide, M. Olson, S. Jothy, K. V. Holmes, and N. Beauchemin Ceacam1a-/- Mice Are Completely Resistant to Infection by Murine Coronavirus Mouse Hepatitis Virus A59 J. Virol., September 15, 2004; 78(18): 10156 - 10165. [Abstract] [Full Text] [PDF]

				U. Sundberg, N. Beauchemin, and B. Obrink The cytoplasmic domain of CEACAM1-L controls its lateral localization and the organization of desmosomes in polarized epithelial cells J. Cell Sci., March 1, 2004; 117(7): 1091 - 1104. [Abstract] [Full Text] [PDF]

				H. S. Miura, K. Nakagaki, and F. Taguchi N-Terminal Domain of the Murine Coronavirus Receptor CEACAM1 Is Responsible for Fusogenic Activation and Conformational Changes of the Spike Protein J. Virol., January 1, 2004; 78(1): 216 - 223. [Abstract] [Full Text] [PDF]

				C. Houde, S. Roy, N. Leung, D. W. Nicholson, and N. Beauchemin The Cell Adhesion Molecule CEACAM1-L Is a Substrate of Caspase-3-mediated Cleavage in Apoptotic Mouse Intestinal Cells J. Biol. Chem., May 9, 2003; 278(19): 16929 - 16935. [Abstract] [Full Text] [PDF]

				B. D. Zelus, J. H. Schickli, D. M. Blau, S. R. Weiss, and K. V. Holmes Conformational Changes in the Spike Glycoprotein of Murine Coronavirus Are Induced at 37{degrees}C either by Soluble Murine CEACAM1 Receptors or by pH 8 J. Virol., January 15, 2003; 77(2): 830 - 840. [Abstract] [Full Text] [PDF]

				D. Schumann, C.-J. Chen, B. Kaplan, and J. E. Shively Carcinoembryonic Antigen Cell Adhesion Molecule 1 Directly Associates with Cytoskeleton Proteins Actin and Tropomyosin J. Biol. Chem., December 14, 2001; 276(50): 47421 - 47433. [Abstract] [Full Text] [PDF]

				R. Kammerer, D. Stober, B. B. Singer, B. Obrink, and J. Reimann Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 on Murine Dendritic Cells Is a Potent Regulator of T Cell Stimulation J. Immunol., June 1, 2001; 166(11): 6537 - 6544. [Abstract] [Full Text] [PDF]

				S. Sadekova, N. Lamarche-Vane, X. Li, and N. Beauchemin The CEACAM1-L Glycoprotein Associates with the Actin Cytoskeleton and Localizes to Cell-Cell Contact through Activation of Rho-like GTPases Mol. Biol. Cell, January 1, 2000; 11(1): 65 - 77. [Abstract] [Full Text]

				M. Huber, L. Izzi, P. Grondin, C. Houde, T. Kunath, A. Veillette, and N. Beauchemin The Carboxyl-terminal Region of Biliary Glycoprotein Controls Its Tyrosine Phosphorylation and Association with Protein-tyrosine Phosphatases SHP-1 and SHP-2 in Epithelial Cells J. Biol. Chem., January 1, 1999; 274(1): 335 - 344. [Abstract] [Full Text] [PDF]

				D. Finkenzeller, B. Kromer, J. Thompson, and W. Zimmermann cea5, a Structurally Divergent Member of the Murine Carcinoembryonic Antigen Gene Family, Is Exclusively Expressed during Early Placental Development in Trophoblast Giant Cells J. Biol. Chem., December 12, 1997; 272(50): 31369 - 31376. [Abstract] [Full Text] [PDF]

				C.-J. Chen, T.-T. Lin, and J. E. Shively Role of Interferon Regulatory Factor-1 in the Induction of Biliary Glycoprotein (Cell CAM-1) by Interferon-{gamma} J. Biol. Chem., November 8, 1996; 271(45): 28181 - 28188. [Abstract] [Full Text] [PDF]

				M. Edlund, I. Blikstad, and B. obrink Calmodulin Binds to Specific Sequences in the Cytoplasmic Domain of C-CAM and Down-regulates C-CAM Self-association J. Biol. Chem., January 19, 1996; 271(3): 1393 - 1399. [Abstract] [Full Text] [PDF]

				I. Hunter, M. Lindh, and B. Obrink Differential regulation of C-CAM isoforms in epithelial cells J. Cell Sci., May 1, 1994; 107(5): 1205 - 1216. [Abstract] [PDF]