CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yehiely, F.
Right arrow Articles by Oren, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yehiely, F.
Right arrow Articles by Oren, M.

Cell Growth & Differentiation, Vol 3, Issue 11 803-809, Copyright © 1992 by American Association of Cancer Research


ARTICLES

The gene for the rat heat-shock cognate, hsc70, can suppress oncogene-mediated transformation

F Yehiely and M Oren
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.

In cells transformed by mutant mouse p53 plus ras, the former protein is found to be complexed with the heat-shock protein cognate hsc70. To determine whether hsc70 can directly affect neoplastic transformation, nonestablished rat embryo fibroblasts (REF) were transfected with rat genomic hsc70 DNA in conjunction with various oncogenes. We report here that the hsc70 gene could efficiently suppress focus induction by mutant p53 plus ras, as well as by myc plus ras. No inhibitory effect of hsc70 was detectable in assays monitoring the ability of REF to be immortalized by mutant p53, arguing against a nonspecific deleterious effect of the hsc70 genomic clone on REF survival and proliferation. Lines generated in the presence of the hsc70 plasmid produced augmented levels of hsc70. Plasmids encoding only short NH2-terminal fragments of hsc70 could also, in some cases, partially reduce oncogene-mediated focus formation. However, a maximal inhibitory effect required the production of a functional hsc70 protein. The data presented here raise the possibility that hsc70 may be directly involved in the modulation of oncogene-mediated transformation.


This article has been cited by other articles:


Home page
MCPHome page
R. D. Unwin, A. Pierce, R. B. Watson, D. W. Sternberg, and A. D. Whetton
Quantitative Proteomic Analysis Using Isobaric Protein Tags Enables Rapid Comparison of Changes in Transcript and Protein Levels in Transformed Cells
Mol. Cell. Proteomics, July 1, 2005; 4(7): 924 - 935.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
F. Buttitta, C. Martella, F. Barassi, L. Felicioni, S. Salvatore, S. Rosini, T. D'Antuono, A. Chella, F. Mucilli, R. Sacco, et al.
Int6 Expression Can Predict Survival in Early-Stage Non-Small Cell Lung Cancer Patients
Clin. Cancer Res., May 1, 2005; 11(9): 3198 - 3204.
[Abstract] [Full Text] [PDF]


Home page
Microbiol. Mol. Biol. Rev.Home page
C. S. Sullivan and J. M. Pipas
T Antigens of Simian Virus 40: Molecular Chaperones for Viral Replication and Tumorigenesis
Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 179 - 202.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Akakura, M. Yoshida, Y. Yoneda, and S. Horinouchi
A Role for Hsc70 in Regulating Nucleocytoplasmic Transport of a Temperature-sensitive p53 (p53Val-135)
J. Biol. Chem., May 4, 2001; 276(18): 14649 - 14657.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Hansen, C. A. Midgley, D. P. Lane, B. C. Freeman, R. I. Morimoto, and T. R. Hupp
Modification of Two Distinct COOH-terminal Domains Is Required for Murine p53 Activation by Bacterial Hsp70
J. Biol. Chem., November 29, 1996; 271(48): 30922 - 30928.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Akakura, M. Yoshida, Y. Yoneda, and S. Horinouchi
A Role for Hsc70 in Regulating Nucleocytoplasmic Transport of a Temperature-sensitive p53 (p53Val-135)
J. Biol. Chem., May 4, 2001; 276(18): 14649 - 14657.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1992 by the American Association of Cancer Research.