Cell Growth & Differentiation, Vol 3, Issue 10 685-691, Copyright © 1992 by American Association of Cancer Research

ARTICLES

Analysis of glutathione transferase P gene regulation with liver cells in primary culture

S Morimura, A Okuda, M Sakai, M Imagawa and M Muramatsu
Department of Biochemistry, University of Tokyo Faculty of Medicine, Japan.

Glutathione transferase P (GST-P) gene is specifically and highly activated during rat chemical hepatocarcinogenesis. We have previously cloned the GST-P gene and have identified putative regulatory regions. To further explore regulatory mechanisms, deletion constructs of the GST-P gene fused to the chloramphenicol acetyltransferase (CAT) structural gene were introduced into primary cultured rat hepatocytes by electroporation, and their activity was determined. The expression of the GST-P-CAT fusion gene is quite low in these cells as compared to that in both a rat fibroblast cell line, 3Y1 cells, and a rat hepatoma cell line, dRLh84. The presence of the strong enhancer GPEI did not elicit any enhancing activity at its original position, or when it was located 3' of the CAT gene, although this element does enhance CAT activity significantly when located adjacent to the promoter. Cotransfection of neither c-jun nor c-fos expression vector, nor both vectors, could enhance the CAT activity, even though GPEI consists of two phorbol ester response element-like sites. Furthermore, the expression of jun family gene was not correlated with GST-P gene expression either in primary cultured hepatocytes or in hepatoma cell lines.

This article has been cited by other articles:

				H. Wang, D. Gilham, and R. Lehner Proteomic and Lipid Characterization of Apolipoprotein B-free Luminal Lipid Droplets from Mouse Liver Microsomes: IMPLICATIONS FOR VERY LOW DENSITY LIPOPROTEIN ASSEMBLY J. Biol. Chem., November 9, 2007; 282(45): 33218 - 33226. [Abstract] [Full Text] [PDF]