CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boise, L. H.
Right arrow Articles by Westin, E. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boise, L. H.
Right arrow Articles by Westin, E. H.

Cell Growth & Differentiation, Vol 3, Issue 1 53-61, Copyright © 1992 by American Association of Cancer Research

ARTICLES

Altered expression of cell cycle related genes including c-myb in a subclone of HL-60 that exhibits reversible differentiation

LH Boise, S Grant and EH Westin
Department of Pharmacology/Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.

A differentiation resistant subclone of HL-60, DMSOr, was removed from the selective pressure of dimethyl sulfoxide and characterized with a new stable phenotype of reversible differentiation. DMSOr cells, when treated with 1.25% dimethyl sulfoxide, differentiated in a manner similar to the parental HL-60 with respect to morphological changes, increase in superoxide production, and withdrawal from cell cycle. Upon removal of the dimethyl sulfoxide at points normally associated with commitment to terminal differentiation, DMSOr reverted to the immature phenotype. This demonstrates an uncoupling of the morphological, functional, and antiproliferative effects of differentiation from commitment to terminal differentiation. Associated with the reversible phenotype of DMSOr was an altered expression of the c-myb oncogene. In HL-60, c-myb expression was down-regulated by 72 h and completely diminished by 144 h. Northern blot analysis of DMSOr demonstrated greater levels of expression of c-myb at 72 and 144 h. Similar results were shown with histone H4, cdc2 kinase, and, to a lesser extent, ornithine decarboxylase. The c-myb related gene B-myb did not show altered regulation during differentiation. The results suggest that altered expression of genes that control cell cycle may be critical for the reversible phenotype of DMSOr.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. M. Luchetti, P. Paroncini, P. Majlingova, J. Frampton, M. Mucenski, S. S. Baroni, P. Sambo, J. Golay, M. Introna, and A. Gabrielli
Characterization of the c-Myb-responsive Region and Regulation of the Human Type I Collagen alpha 2 Chain Gene by c-Myb
J. Biol. Chem., January 10, 2003; 278(3): 1533 - 1541.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1992 by the American Association of Cancer Research.