CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Daly, R. J.
Right arrow Articles by Darbre, P. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Daly, R. J.
Right arrow Articles by Darbre, P. D.

Cell Growth & Differentiation, Vol 2, Issue 9 457-464, Copyright © 1991 by American Association of Cancer Research


ARTICLES

Autocrine production of insulin-like growth factor II using an inducible expression system results in reduced estrogen sensitivity of MCF-7 human breast cancer cells

RJ Daly, WH Harris, DY Wang and PD Darbre
Department of Pharmacology, New York University Medical Center, New York 10016.

An inducible expression vector, utilizing the metal response elements from the human metallothionein IIA promoter and encoding human prepro-insulin-like growth factor II (IGF-II), was transfected into MCF-7 McG cells, an MCF-7 subline which exhibits an estrogen dependent phenotype in vitro and does not express detectable levels of IGF-II mRNA. Two stably transfected clones, designated MI5 and MI7, which expressed IGF-II mRNA in a Zn2+ regulated manner, were isolated. Clone MI5 did not secrete detectable levels of IGF-II activity, as determined by radioimmunoassay of conditioned medium, but clone MI7 secreted high levels of IGF-II activity in a Zn2+ inducible fashion. Clone MI5 and control clones transfected with the selection plasmid pSV2 neo and the control plasmid pSP65 continued to display an estrogen dependent phenotype in vitro. However, under both anchorage dependent and anchorage independent growth conditions, clone MI7 cells exhibited an estrogen responsive, rather than dependent, phenotype. Moreover, when grown in the presence of inducing concentrations of Zn2+, MI7 cells were either virtually (for anchorage dependent growth) or completely (anchorage independent growth) unresponsive to estradiol. Both the basal growth rate in the absence of metal ions and the Zn2+ induced increases in cell proliferation could be inhibited by the monoclonal antibody alpha-IR3, which blocks the binding site of the IGF-I receptor. The antiestrogens tamoxifen and 4-hydroxytamoxifen were found to enhance the growth stimulation resulting from Zn2+ induced IGF-II production in MI7 cells.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
Endocr Relat CancerHome page
C. M Staka, R. I Nicholson, and J. M W Gee
Acquired resistance to oestrogen deprivation: role for growth factor signalling kinases/oestrogen receptor cross-talk revealed in new MCF-7X model
Endocr. Relat. Cancer, July 1, 2005; 12(Supplement_1): S85 - S97.
[Abstract] [Full Text] [PDF]


Home page
Endocr Relat CancerHome page
R I Nicholson, C Staka, F Boyns, I R Hutcheson, and J M W Gee
Growth factor-driven mechanisms associated with resistance to estrogen deprivation in breast cancer: new opportunities for therapy
Endocr. Relat. Cancer, December 1, 2004; 11(4): 623 - 641.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page

Clin. Cancer Res., January 1, 2004; 10(1): 346s - 354s.



Home page
J Biol ChemHome page
R. L. Stephen, L. E. Shaw, C. Larsen, D. Corcoran, and P. D. Darbre
Insulin-like Growth Factor Receptor Levels Are Regulated by Cell Density and by Long Term Estrogen Deprivation in MCF7 Human Breast Cancer Cells
J. Biol. Chem., October 26, 2001; 276(43): 40080 - 40086.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. Lu and G. Serrero
Mediation of estrogen mitogenic effect in human breast cancer MCF-7 cells by PC-cell-derived growth factor (PCDGF/granulin precursor)
PNAS, January 2, 2001; 98(1): 142 - 147.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
E. J. Goetzl, H. Dolezalova, Y. Kong, and L. Zeng
Dual Mechanisms for Lysophospholipid Induction of Proliferation of Human Breast Carcinoma Cells
Cancer Res., September 1, 1999; 59(18): 4732 - 4737.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
O. W. J. Prall, E. M. Rogan, E. A. Musgrove, C. K. W. Watts, and R. L. Sutherland
c-Myc or Cyclin D1 Mimics Estrogen Effects on Cyclin E-Cdk2 Activation and Cell Cycle Reentry
Mol. Cell. Biol., August 1, 1998; 18(8): 4499 - 4508.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. Lu and G. Serrero
Mediation of estrogen mitogenic effect in human breast cancer MCF-7 cells by PC-cell-derived growth factor (PCDGF/granulin precursor)
PNAS, December 22, 2000; (2000) 11525198.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1991 by the American Association of Cancer Research.