CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by den Hertog, J.
Right arrow Articles by Kruijer, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by den Hertog, J.
Right arrow Articles by Kruijer, W.

Cell Growth & Differentiation, Vol 2, Issue 3 155-164, Copyright © 1991 by American Association of Cancer Research


ARTICLES

Neuronal differentiation in response to epidermal growth factor of transfected murine P19 embryonal carcinoma cells expressing human epidermal growth factor receptors

J den Hertog, SW de Laat, J Schlessinger and W Kruijer
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.

The human epidermal growth factor receptor (hEGF-R) was introduced into murine P19 embryonal carcinoma (EC) cells, which do not express endogenous EGF-R. Undifferentiated stable P19 EC transfectants containing multiple copies of the hEGF-R complementary DNA were isolated. These cells express functional EGF-R, exhibiting characteristic biphasic EGF binding and intrinsic tyrosine protein kinase activity. Whereas normally EGF induces the expression of multiple nuclear protooncogenes, only junB expression is induced by EGF in the HER-transfected cells. This indicates that undifferentiated P19 EC cells contain at least part of a signal transduction machinery capable of coupling to the ectopically expressed hEGF-R. Interestingly, neuronal differentiation is induced in these cells in response to EGF under culture conditions resembling those during early preimplantation embryogenesis. These results indicate that neuronal differentiation of pluripotent P19 EC cells can be induced via activation of a tyrosine protein kinase signaling pathway.


This article has been cited by other articles:


Home page
J Biol ChemHome page
A. Buist, C. Blanchetot, L. G. J. Tertoolen, and J. den Hertog
Identification of p130cas as an in VivoSubstrate of Receptor Protein-tyrosine Phosphatase {alpha}
J. Biol. Chem., July 7, 2000; 275(27): 20754 - 20761.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
O Silvennoinen, C Schindler, J Schlessinger, and D. Levy
Ras-independent growth factor signaling by transcription factor tyrosine phosphorylation
Science, September 24, 1993; 261(5129): 1736 - 1739.
[Abstract] [PDF]


Home page
J Biol ChemHome page
A. Buist, C. Blanchetot, L. G. J. Tertoolen, and J. den Hertog
Identification of p130cas as an in VivoSubstrate of Receptor Protein-tyrosine Phosphatase {alpha}
J. Biol. Chem., July 7, 2000; 275(27): 20754 - 20761.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1991 by the American Association of Cancer Research.