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Cell Growth & Differentiation, Vol 2, Issue 2 77-83, Copyright © 1991 by American Association of Cancer Research


ARTICLES

Cell lineage specificity of expression of the murine transforming growth factor beta 3 and transforming growth factor beta 1 genes

F Watrin, L Scotto, RK Assoian and DJ Wolgemuth
Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032.

We have cloned a murine transforming growth factor (TGF) beta 3 complementary (cDNA) from normal tissue by low stringency screening of a testicular cDNA library with a TGF beta 1 probe. The coding domain of this TGF beta 3 cDNA agrees completely with the sequence reported for the TGF beta 3 cDNA isolated from the AKR-2B cell line, but the testicular clone uses a distinct and unusual polyadenylation signal resulting in an altered 3' untranslated domain. Northern blot hybridization analysis of gonadal tissues showed that both TGF beta 3 and TGF beta 1 mRNAs are detectable in the mouse testis and ovary. A detailed analysis of TGF beta 3 and TGF beta 1 gene expression in normal and germ cell-deficient male mice showed that the somatic cell compartment of the mouse testis expresses the usual-sized transcripts for both genes. However, a smaller (1.8-kilobase) TGF beta 1 mRNA is expressed selectively in male germ cells, and expression of this transcript was constitutive throughout the spermatogenic stages examined. This result demonstrates a new pattern of TGF beta 1 gene expression, consistent with cell lineage-specific transcriptional regulation during spermatogenesis.


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A Wagener, J Fickel, J Schon, A Fritzenkotter, F Goritz, and S Blottner
Seasonal variation in expression and localization of testicular transforming growth factors TGF-{beta}1 and TGF-{beta}3 corresponds with spermatogenic activity in roe deer
J. Endocrinol., November 1, 2005; 187(2): 205 - 215.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1991 by the American Association of Cancer Research.