Cell Growth & Differentiation, Vol 2, Issue 12 661-667, Copyright © 1991 by American Association of Cancer Research

ARTICLES

Involvement of wild-type p53 protein in the cell cycle requires nuclear localization

G Shaulsky, N Goldfinger, A Peled and V Rotter
Department of Cell Biology, Weizmann Institute of Science, Rehovot, Israel.

Transfection of wild-type p53 into a pre-B, p53 nonproducer cell line yielded the generation of stable clones. Although constitutively expressing the growth-suppressor wild-type p53 protein, these cells proliferate continuously in vitro. However, expression of wild-type p53 in these cells altered their cell cycle pattern and reduced their growth in vivo. When the same parental cells were transfected with a plasmid coding for a wild-type p53 lacking nuclear localization signals, a wild-type cytoplasmic p53 protein was expressed. Expression of this cytoplasmic p53 product did not exert any changes in the growth of the parental cells, suggesting that wild-type p53 affects the cell cycle only when localized in the nuclear cell compartment.

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