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Cell Growth & Differentiation, Vol 2, Issue 10 495-501, Copyright © 1991 by American Association of Cancer Research


ARTICLES

Identification and cloning of a novel amplified DNA sequence in human malignant fibrous histiocytoma derived from a region of chromosome 12 frequently rearranged in soft tissue tumors

PS Meltzer, SA Jankowski, P Dal Cin, AA Sandberg, IB Paz and MA Coccia
Department of Pediatrics, University of Michigan, Ann Arbor 48109.

Amplification of cellular oncogenes occurs frequently in several human cancers and is an important mechanism of increased gene expression. Identification of amplified genes in tumor cells has proved to be a useful approach for understanding genetic alterations in cancer. Previous procedures for isolating probes from amplified DNA sequences have relied on tissue culture cells, limiting the range of tumors that can be studied and raising questions of in vitro artifact. We have circumvented these problems by combining in gel renaturation of amplified sequences with the polymerase chain reaction. Using this approach, we have identified and partially cloned a DNA amplification unit from biopsies of human malignant fibrous histiocytoma. This amplification unit is derived from chromosome 12q13-14, a site commonly involved in rearrangements in soft tissue tumors, and contains at least one transcribed region (designated SAS, for sarcoma amplified sequence).


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J Biol ChemHome page
M. I. Gallego and P. A. Lazo
Deletion in Human Chromosome Region 12q13-15 by Integration of Human Papillomavirus DNA in a Cervical Carcinoma Cell Line
J. Biol. Chem., October 13, 1995; 270(41): 24321 - 24326.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1991 by the American Association of Cancer Research.