Cell Growth & Differentiation, Vol 2, Issue 1 43-49, Copyright © 1991 by American Association of Cancer Research

ARTICLES

Expression of the jun-B gene during induction of monocytic differentiation

R Datta, ML Sherman, RM Stone and D Kufe
Laboratory of Clinical Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.

The AP-1 protein complex binds to specific DNA sequences that regulate transcription of genes responsive to certain growth factors and phorbol esters. This complex is composed of products of the jun and fos gene families. The present studies have examined the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) on the regulation of jun-B gene expression during induction of monocytic differentiation. Low levels of jun-B transcripts were present in uninduced HL-60 promyelocytic leukemia cells. In contrast, treatment with TPA was associated with rapid increases in jun-B mRNA levels that were maximal at 3 h and remained elevated at 48 h. The induction of jun-B expression by TPA in these cells preceded that of the c-jun and c-fos genes. Similar increases in jun-B transcripts were detectable in TPA-treated THP-1 and U-937 myeloid leukemia cells, although expression of this gene was transient in the more differentiated THP-1 cells. Run-on assays demonstrated low levels of jun-B gene activation in untreated HL-60 cells, whereas TPA treatment was associated with a 6-fold increase in the transcription rate of this gene. This induction of jun-B expression occurred in the absence of de novo protein synthesis. In contrast, inhibition of protein synthesis was associated with superinduction of TPA-induced jun-B mRNA levels and an increase in stability of this transcript. These findings suggest that jun-B gene expression is regulated at both the transcriptional and posttranscriptional levels during induction of monocytic differentiation.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				S. Baron, A. Escande, G. Alberola, K. Bystricky, P. Balaguer, and H. Richard-Foy Estrogen Receptor {alpha} and the Activating Protein-1 Complex Cooperate during Insulin-like Growth Factor-I-induced Transcriptional Activation of the pS2/TFF1 Gene J. Biol. Chem., April 20, 2007; 282(16): 11732 - 11741. [Abstract] [Full Text] [PDF]

				C. Grant, P. Jain, M. Nonnemacher, K. E. Flaig, B. Irish, J. Ahuja, A. Alexaki, T. Alefantis, and B. Wigdahl AP-1-directed human T cell leukemia virus type 1 viral gene expression during monocytic differentiation J. Leukoc. Biol., September 1, 2006; 80(3): 640 - 650. [Abstract] [Full Text] [PDF]

				X. Wang and G. P. Studzinski The Requirement for and Changing Composition of the Activating Protein-1 Transcription Factor during Differentiation of Human Leukemia HL60 Cells Induced by 1,25-Dihydroxyvitamin D3. Cancer Res., April 15, 2006; 66(8): 4402 - 4409. [Abstract] [Full Text] [PDF]

				L. P. Freedman Transcriptional Targets of the Vitamin D3 Receptor–Mediating Cell Cycle Arrest and Differentiation J. Nutr., February 1, 1999; 129(2): 581 - 581. [Abstract] [Full Text] [PDF]

				M. Kaneki, S. Kharbanda, P. Pandey, K. Yoshida, M. Takekawa, J.-R. Liou, R. Stone, and D. Kufe Functional Role for Protein Kinase Cbeta as a Regulator of Stress-Activated Protein Kinase Activation and Monocytic Differentiation of Myeloid Leukemia Cells Mol. Cell. Biol., January 1, 1999; 19(1): 461 - 470. [Abstract] [Full Text] [PDF]

				M Liu, M H Lee, M Cohen, M Bommakanti, and L P Freedman Transcriptional activation of the Cdk inhibitor p21 by vitamin D3 leads to the induced differentiation of the myelomonocytic cell line U937. Genes & Dev., January 15, 1996; 10(2): 142 - 153. [Abstract] [PDF]