CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wilson, H.-M. P.
Right arrow Articles by Swisshelm, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilson, H.-M. P.
Right arrow Articles by Swisshelm, K.
Cell Growth & Differentiation Vol. 13, 205-213, May 2002
© 2002 American Association for Cancer Research

Insulin-like Growth Factor Binding Protein-related Protein 1 Inhibits Proliferation of MCF-7 Breast Cancer Cells via a Senescence-like Mechanism1

Heather-Marie P. Wilson, Roger S. Birnbaum, Martin Poot, LeBris S. Quinn and Karen Swisshelm2

Department of Pathology, University of Washington School of Medicine, Seattle, Washington 98195 [H-M. P. W., M. P., K. S.]; Research Service and Geriatric Research, Education, and Clinical Center, Veterans Administration Puget Sound Health Care System, Tacoma, Washington 98493 [R. S. B.]; and Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Washington, Seattle, Washington 98195 [R. S. B., L. S. Q.]

Elevated insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) mRNA in senescent human mammary epithelial cells suggested that the IGFBP-3 gene product may inhibit cell proliferation. To test this hypothesis, we used a retroviral vector to express IGFBP-rP1 cDNA in the IGFBP-rP1-deficient MCF-7 breast cancer cell line. Compared with control vector-transduced cells, cumulative cell numbers for IGFBP-rP1-transduced polyclonal or clonal cell cultures were reduced by 39 and 74%, respectively, after 1 week. Medium conditioned by IGFBP-rP1-producing cultures reduced cumulative cell numbers in parental MCF-7 cultures by 20% compared with medium from cultures of a control vector-transduced cell line. Nuclear fragmentation analysis and cell proliferation assays completed in the presence of the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone excluded apoptosis as the responsible mechanism. The percentage of cells containing senescence-associated ß-galactosidase activity was doubled compared with control cell cultures. Flow cytometry analysis indicated that twice as many noncycling cells were present in the IGFBP-rP1-transduced MCF-7 cell cultures compared with controls. These findings indicate that IGFBP-rP1 is an inhibitor of MCF-7 breast cancer cell proliferation and may act via a cellular senescence-like mechanism.




This article has been cited by other articles:


Home page
Am. J. Physiol. Renal Physiol.Home page
T. Matsumoto, S. Hess, H. Kajiyama, T. Sakairi, M. A. Saleem, P. W. Mathieson, Y. Nojima, and J. B. Kopp
Proteomic analysis identifies insulin-like growth factor-binding protein-related protein-1 as a podocyte product
Am J Physiol Renal Physiol, October 1, 2010; 299(4): F776 - F784.
[Abstract] [Full Text] [PDF]


Home page
RNAHome page
W. M. Gommans, N. E. Tatalias, C. P. Sie, D. Dupuis, N. Vendetti, L. Smith, R. Kaushal, and S. Maas
Screening of human SNP database identifies recoding sites of A-to-I RNA editing
RNA, October 1, 2008; 14(10): 2074 - 2085.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
X.-Y. Sun, F.-X. Li, J. Li, Y.-F. Tan, Y.-S. Piao, S. Tang, and Y.-L. Wang
Determination of Genes Involved in the Early Process of Embryonic Implantation in Rhesus Monkey (Macaca mulatta) by Suppression Subtractive Hybridization
Biol Reprod, May 1, 2004; 70(5): 1365 - 1373.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
I. B. Roninson
Tumor Cell Senescence in Cancer Treatment
Cancer Res., June 1, 2003; 63(11): 2705 - 2715.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2002 by the American Association of Cancer Research.