This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Flory, M. R. Articles by Davis, T. N. Search for Related Content PubMed PubMed Citation Articles by Flory, M. R. Articles by Davis, T. N.

Pcp1p, an Spc110p-related Calmodulin Target at the Centrosome of the Fission Yeast Schizosaccharomyces pombe¹

Molecular and Cellular Biology Program [M. R. F., T. N. D.] and Department of Biochemistry [T. N. D.], University of Washington, Seattle, Washington 98195; Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309 [M. M.]; and Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710 [J. D. J., A. R. M.]

In the budding yeast Saccharomyces cerevisiae, the calmodulin-binding protein Spc110p/Nuf1p facilitates mitotic spindle formation from the fungal centrosome or spindle pole body (SPB). The human Spc110p orthologue kendrin is a centrosomal, calmodulin-binding pericentrin isoform that is specifically overexpressed in carcinoma cells. Here we establish an evolutionary and functional link between Spc110p and kendrin through identification and analysis of similar calmodulin-binding proteins in the fission yeast Schizosaccharomyces pombe (Pcp1p, pole target of calmodulin in S. pombe) and the filamentous fungus Aspergillus nidulans. Like Spc110p and kendrin, Pcp1p and the A. nidulans protein contain predicted coiled-coil secondary structure and a COOH-terminal calmodulin-binding region. Green fluorescent protein fusions of Pcp1p localize to the SPB as analyzed by fluorescence and immunoelectron microscopy. Pcp1p overexpression causes chromosome missegregation, multiple mitotic spindle fragments, and multiple abnormal SPB-like structures, a phenotype remarkably similar to that of many human carcinoma lines, which exhibit chromosome and spindle defects, and supernumerary centrosomes.

This article has been cited by other articles:

				K.-W. Fong, Y.-K. Choi, J. B. Rattner, and R. Z. Qi CDK5RAP2 Is a Pericentriolar Protein That Functions in Centrosomal Attachment of the {gamma}-Tubulin Ring Complex Mol. Biol. Cell, January 1, 2008; 19(1): 115 - 125. [Abstract] [Full Text] [PDF]

				J. E. Sillibourne, B. Delaval, S. Redick, M. Sinha, and S. J. Doxsey Chromatin Remodeling Proteins Interact with Pericentrin to Regulate Centrosome Integrity Mol. Biol. Cell, September 1, 2007; 18(9): 3667 - 3680. [Abstract] [Full Text] [PDF]

				E. L. Grishchuk, I. S. Spiridonov, and J. R. McIntosh Mitotic Chromosome Biorientation in Fission Yeast Is Enhanced by Dynein and a Minus-end-directed, Kinesin-like Protein Mol. Biol. Cell, June 1, 2007; 18(6): 2216 - 2225. [Abstract] [Full Text] [PDF]

				C. Wiese and Y. Zheng Microtubule nucleation: {gamma}-tubulin and beyond J. Cell Sci., October 15, 2006; 119(20): 4143 - 4153. [Abstract] [Full Text] [PDF]

				J. A. Rosenberg, G. C. Tomlin, W. H. McDonald, B. E. Snydsman, E. G. Muller, J. R. Yates III, and K. L. Gould Ppc89 Links Multiple Proteins, Including the Septation Initiation Network, to the Core of the Fission Yeast Spindle-Pole Body Mol. Biol. Cell, September 1, 2006; 17(9): 3793 - 3805. [Abstract] [Full Text] [PDF]

				I. Samejima, P. C. C. Lourenco, H. A. Snaith, and K. E. Sawin Fission Yeast mto2p Regulates Microtubule Nucleation by the Centrosomin-related Protein mto1p Mol. Biol. Cell, June 1, 2005; 16(6): 3040 - 3051. [Abstract] [Full Text] [PDF]

				L. L. Freeman-Cook, E. B. Gomez, E. J. Spedale, J. Marlett, S. L. Forsburg, L. Pillus, and P. Laurenson Conserved Locus-Specific Silencing Functions of Schizosaccharomyces pombe sir2+ Genetics, March 1, 2005; 169(3): 1243 - 1260. [Abstract] [Full Text] [PDF]

				W. C. Zimmerman, J. Sillibourne, J. Rosa, and S. J. Doxsey Mitosis-specific Anchoring of {gamma} Tubulin Complexes by Pericentrin Controls Spindle Organization and Mitotic Entry Mol. Biol. Cell, August 1, 2004; 15(8): 3642 - 3657. [Abstract] [Full Text] [PDF]

				S. Venkatram, J. J. Tasto, A. Feoktistova, J. L. Jennings, A. J. Link, and K. L. Gould Identification and Characterization of Two Novel Proteins Affecting Fission Yeast {gamma}-tubulin Complex Function Mol. Biol. Cell, May 1, 2004; 15(5): 2287 - 2301. [Abstract] [Full Text] [PDF]

				C. R. Kahl and A. R. Means Regulation of Cell Cycle Progression by Calcium/Calmodulin-Dependent Pathways Endocr. Rev., December 1, 2003; 24(6): 719 - 736. [Abstract] [Full Text] [PDF]