CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lang, S. H.
Right arrow Articles by Maitland, N. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lang, S. H.
Right arrow Articles by Maitland, N. J.
Cell Growth & Differentiation Vol. 12, 631-640, December 2001
© 2001 American Association for Cancer Research

Experimental Prostate Epithelial Morphogenesis in Response to Stroma and Three-Dimensional Matrigel Culture1

Shona H. Lang2, Meg Stark, Anne Collins, Alan B. Paul, Michael J. Stower and Norman J. Maitland

YCR Cancer Research Unit [S. H. L., N. J. M.], Department of Biology [M. S.], University of York, Heslington, York YO10 5YW; Prostate Research Group, Department of Surgery, The Medical School, University of Newcastle, Newcastle Upon Tyne NE2 4HH, United Kingdom [A. C.]; Pyrah Department of Urology, St. James Hospital, Leeds LS9 7TF [A. B. P.]; and Department of Urology, York District Hospital, York YO31 8HE [M. J. S.], United Kingdom

To reproduce the structural and functional differentiation of human prostatic acini in vivo, prostatic epithelial and stromal cells derived from human primary cultures were cocultured in Matrigel. In the absence of stroma and serum, epithelial spheroids composed of solid masses of stratified and cuboidal cells formed. Outer cells of the spheroid expressed cytokeratins 1, 5, 10, and 14, whereas the inner cells expressed cytokeratin 18. The addition of 2% serum induced formation of a lumen surrounded by a layer of one or two cuboidal and columnar epithelial cells. The further addition of stromal cultures, dihydrotestosterone, and estrogen induced polarization of the epithelium and increased spheroid-forming efficiency. Epithelium expressed either cytokeratin 18 alone or additionally cytokeratins 1, 5, 14, and 10. All spheroid epithelium expressed prostate-specific antigen and prostate-specific membrane antigen. Androgen receptor was only detected in the presence of stroma, serum, and hormones. Thus, development of a functional and morphologically correct prostate gland in vitro is dependent on extracellular matrix, steroid hormones, and factors from stromal cells and serum.




This article has been cited by other articles:


Home page
Endocr Relat CancerHome page
N. Sampson, H. Neuwirt, M. Puhr, H. Klocker, and I. E. Eder
In vitro model systems to study androgen receptor signaling in prostate cancer
Endocr. Relat. Cancer, March 26, 2013; 20(2): R49 - R64.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
C.-P. Liao, H. Adisetiyo, M. Liang, and P. Roy-Burman
Cancer-Associated Fibroblasts Enhance the Gland-Forming Capability of Prostate Cancer Stem Cells
Cancer Res., September 15, 2010; 70(18): 7294 - 7303.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
G. V. Shah, A. Muralidharan, M. Gokulgandhi, K. Soan, and S. Thomas
Cadherin Switching and Activation of {beta}-Catenin Signaling Underlie Proinvasive Actions of Calcitonin-Calcitonin Receptor Axis in Prostate Cancer
J. Biol. Chem., January 9, 2009; 284(2): 1018 - 1030.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Renal Physiol.Home page
S. K. Addla, M. D. Brown, C. A. Hart, V. A. C. Ramani, and N. W. Clarke
Characterization of the Hoechst 33342 side population from normal and malignant human renal epithelial cells
Am J Physiol Renal Physiol, September 1, 2008; 295(3): F680 - F687.
[Abstract] [Full Text] [PDF]


Home page
CarcinogenesisHome page
X. Liu, J. D. Allen, J. T. Arnold, and M. R. Blackman
Lycopene inhibits IGF-I signal transduction and growth in normal prostate epithelial cells by decreasing DHT-modulated IGF-I production in co-cultured reactive stromal cells
Carcinogenesis, April 1, 2008; 29(4): 816 - 823.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
J. Miki, B. Furusato, H. Li, Y. Gu, H. Takahashi, S. Egawa, I. A. Sesterhenn, D. G. McLeod, S. Srivastava, and J. S. Rhim
Identification of Putative Stem Cell Markers, CD133 and CXCR4, in hTERT-Immortalized Primary Nonmalignant and Malignant Tumor-Derived Human Prostate Epithelial Cell Lines and in Prostate Cancer Specimens
Cancer Res., April 1, 2007; 67(7): 3153 - 3161.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
I. Kogan, N. Goldfinger, M. Milyavsky, M. Cohen, I. Shats, G. Dobler, H. Klocker, B. Wasylyk, M. Voller, T. Aalders, et al.
hTERT-Immortalized Prostate Epithelial and Stromal-Derived Cells: an Authentic In vitro Model for Differentiation and Carcinogenesis
Cancer Res., April 1, 2006; 66(7): 3531 - 3540.
[Abstract] [Full Text] [PDF]


Home page
Endocr Relat CancerHome page
K-M Fung, E N S Samara, C Wong, A Metwalli, R Krlin, B Bane, C Z Liu, J T Yang, J V Pitha, D J Culkin, et al.
Increased expression of type 2 3{alpha}-hydroxysteroid dehydrogenase/type 5 17{beta}-hydroxysteroid dehydrogenase (AKR1C3) and its relationship with androgen receptor in prostate carcinoma.
Endocr. Relat. Cancer, March 1, 2006; 13(1): 169 - 180.
[Abstract] [Full Text] [PDF]


Home page
Endocr Relat CancerHome page
D M Peehl
Primary cell cultures as models of prostate cancer development
Endocr. Relat. Cancer, March 1, 2005; 12(1): 19 - 47.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
G. D. Richardson, C. N. Robson, S. H. Lang, D. E. Neal, N. J. Maitland, and A. T. Collins
CD133, a novel marker for human prostatic epithelial stem cells
J. Cell Sci., July 15, 2004; 117(16): 3539 - 3545.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. Futaki, Y. Hayashi, M. Yamashita, K. Yagi, H. Bono, Y. Hayashizaki, Y. Okazaki, and K. Sekiguchi
Molecular Basis of Constitutive Production of Basement Membrane Components: GENE EXPRESSION PROFILES OF ENGELBRETH-HOLM-SWARM TUMOR AND F9 EMBRYONAL CARCINOMA CELLS
J. Biol. Chem., December 12, 2003; 278(50): 50691 - 50701.
[Abstract] [Full Text] [PDF]


Home page
Mol Cancer ResHome page
C. P. Tran, C. Lin, J. Yamashiro, and R. E. Reiter
Prostate Stem Cell Antigen Is a Marker of Late Intermediate Prostate Epithelial Cells
Mol. Cancer Res., December 1, 2002; 1(2): 113 - 121.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2001 by the American Association of Cancer Research.