CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denis, G. V.
Right arrow Articles by Faller, D. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denis, G. V.
Right arrow Articles by Faller, D. V.
Cell Growth & Differentiation Vol. 11, 417-424, August 2000
© 2000 American Association for Cancer Research

RING3 Kinase Transactivates Promoters of Cell Cycle Regulatory Genes through E2F1

Gerald V. Denis2, Cyrus Vaziri, Ning Guo and Douglas V. Faller

Cancer Research Center, Boston University School of Medicine, Boston, Massachusetts 02118

RING3 is a novel, nuclear-localized, serine-threonine kinase that has elevated activity in human leukemias. RING3 transforms NIH/3T3 cells and is activated by mitogenic signals, all of which suggest that it may play a role in cell cycle-responsive transcription. We tested this hypothesis with transient transfection of RING3 into fibroblasts and assayed transactivation of the promoters of cyclin D1, cyclin A, cyclin E, and dihydrofolate reductase (dhfr) genes. RING3 transactivates these promoters in a manner dependent on ras signaling. A kinase-deficient point mutant of RING3 does not transactivate. Mutational analysis of the dhfr promoter reveals that transactivation also depends on the presence of a functional E2F binding site. Furthermore, ectopic expression of Rb protein, a negative regulator of E2F activity, suppresses the RING3-dependent transactivation of this promoter. Consistent with a potential role of E2F in RING3-dependent transcription, anti-RING3 immunoaffinity chromatography or recombinant RING3 protein affinity chromatography of nuclear extracts copurified a protein complex that contains E2F-1 and E2F-2. These data suggest that RING3 is a potentially important regulator of E2F-dependent cell cycle genes.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Mochizuki, A. Nishiyama, M. K. Jang, A. Dey, A. Ghosh, T. Tamura, H. Natsume, H. Yao, and K. Ozato
The Bromodomain Protein Brd4 Stimulates G1 Gene Transcription and Promotes Progression to S Phase
J. Biol. Chem., April 4, 2008; 283(14): 9040 - 9048.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-Y. Wu and C.-M. Chiang
The Double Bromodomain-containing Chromatin Adaptor Brd4 and Transcriptional Regulation
J. Biol. Chem., May 4, 2007; 282(18): 13141 - 13145.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. E. Lenburg, A. Sinha, D. V. Faller, and G. V. Denis
Tumor-specific and Proliferation-specific Gene Expression Typifies Murine Transgenic B Cell Lymphomagenesis
J. Biol. Chem., February 16, 2007; 282(7): 4803 - 4811.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. N. Davis, K. J. Wojno, S. Daignault, M. D. Hofer, R. Kuefer, M. A. Rubin, and M. L. Day
Elevated E2F1 Inhibits Transcription of the Androgen Receptor in Metastatic Hormone-Resistant Prostate Cancer
Cancer Res., December 15, 2006; 66(24): 11897 - 11906.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Ottinger, T. Christalla, K. Nathan, M. M. Brinkmann, A. Viejo-Borbolla, and T. F. Schulz
Kaposi's Sarcoma-Associated Herpesvirus LANA-1 Interacts with the Short Variant of BRD4 and Releases Cells from a BRD4- and BRD2/RING3-Induced G1 Cell Cycle Arrest
J. Virol., November 1, 2006; 80(21): 10772 - 10786.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S.-Y. Wu, A-Y. Lee, S. Y. Hou, J. K. Kemper, H. Erdjument-Bromage, P. Tempst, and C.-M. Chiang
Brd4 links chromatin targeting to HPV transcriptional silencing
Genes & Dev., September 1, 2006; 20(17): 2383 - 2396.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Viejo-Borbolla, M. Ottinger, E. Bruning, A. Burger, R. Konig, E. Kati, J. A. Sheldon, and T. F. Schulz
Brd2/RING3 Interacts with a Chromatin-Binding Domain in the Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen 1 (LANA-1) That Is Required for Multiple Functions of LANA-1
J. Virol., November 1, 2005; 79(21): 13618 - 13629.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Farina, M. Hattori, J. Qin, Y. Nakatani, N. Minato, and K. Ozato
Bromodomain Protein Brd4 Binds to GTPase-Activating SPA-1, Modulating Its Activity and Subcellular Localization
Mol. Cell. Biol., October 15, 2004; 24(20): 9059 - 9069.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L.-Y. Wong, G. A. Matchett, and A. C. Wilson
Transcriptional Activation by the Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Is Facilitated by an N-Terminal Chromatin-Binding Motif
J. Virol., September 15, 2004; 78(18): 10074 - 10085.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Spira, J. Beane, V. Shah, G. Liu, F. Schembri, X. Yang, J. Palma, and J. S. Brody
Effects of cigarette smoke on the human airway epithelial cell transcriptome
PNAS, July 6, 2004; 101(27): 10143 - 10148.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Taubert, C. Gorrini, S. R. Frank, T. Parisi, M. Fuchs, H.-M. Chan, D. M. Livingston, and B. Amati
E2F-Dependent Histone Acetylation and Recruitment of the Tip60 Acetyltransferase Complex to Chromatin in Late G1
Mol. Cell. Biol., May 15, 2004; 24(10): 4546 - 4556.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. J. Greenwald, J. R. Tumang, A. Sinha, N. Currier, R. D. Cardiff, T. L. Rothstein, D. V. Faller, and G. V. Denis
E{micro}-BRD2 transgenic mice develop B-cell lymphoma and leukemia
Blood, February 15, 2004; 103(4): 1475 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. Canaple, T. Kakizawa, and V. Laudet
The Days and Nights of Cancer Cells
Cancer Res., November 15, 2003; 63(22): 7545 - 7552.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Dey, F. Chitsaz, A. Abbasi, T. Misteli, and K. Ozato
The double bromodomain protein Brd4 binds to acetylated chromatin during interphase and mitosis
PNAS, July 22, 2003; 100(15): 8758 - 8763.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Viejo-Borbolla, E. Kati, J. A. Sheldon, K. Nathan, K. Mattsson, L. Szekely, and T. F. Schulz
A Domain in the C-Terminal Region of Latency-Associated Nuclear Antigen 1 of Kaposi's Sarcoma-Associated Herpesvirus Affects Transcriptional Activation and Binding to Nuclear Heterochromatin
J. Virol., June 15, 2003; 77(12): 7093 - 7100.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Maruyama, A. Farina, A. Dey, J. Cheong, V. P. Bermudez, T. Tamura, S. Sciortino, J. Shuman, J. Hurwitz, and K. Ozato
A Mammalian Bromodomain Protein, Brd4, Interacts with Replication Factor C and Inhibits Progression to S Phase
Mol. Cell. Biol., September 15, 2002; 22(18): 6509 - 6520.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
T. E. Crowley, E. M. Kaine, M. Yoshida, A. Nandi, and D. J. Wolgemuth
Reproductive Cycle Regulation of Nuclear Import, Euchromatic Localization, and Association with Components of Pol II Mediator of a Mammalian Double-Bromodomain Protein
Mol. Endocrinol., August 1, 2002; 16(8): 1727 - 1737.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Houzelstein, S. L. Bullock, D. E. Lynch, E. F. Grigorieva, V. A. Wilson, and R. S. P. Beddington
Growth and Early Postimplantation Defects in Mice Deficient for the Bromodomain-Containing Protein Brd4{dagger}
Mol. Cell. Biol., June 1, 2002; 22(11): 3794 - 3802.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Grundschober, F. Delaunay, A. Puhlhofer, G. Triqueneaux, V. Laudet, T. Bartfai, and P. Nef
Circadian Regulation of Diverse Gene Products Revealed by mRNA Expression Profiling of Synchronized Fibroblasts
J. Biol. Chem., December 7, 2001; 276(50): 46751 - 46758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2000 by the American Association of Cancer Research.