CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Atencio, I. A.
Right arrow Articles by Demers, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atencio, I. A.
Right arrow Articles by Demers, G. W.
Cell Growth & Differentiation Vol. 11, 247-253, May 2000
© 2000 American Association for Cancer Research


Articles

Calpain Inhibitor 1 Activates p53-dependent Apoptosis in Tumor Cell Lines

Isabella A. Atencio1, Murali Ramachandra, Paul Shabram and G. William Demers

Canji, Inc., San Diego, California 92121

Abstract

Reports suggest a role of calpains in degradation of wild-type p53, which may regulate p53 induction of apoptosis. A calpain inhibitor, n-acetyl-leu-leu-norleucinal (calpain inhibitor 1), was assessed for ability to enhance p53-dependent apoptosis in human tumor cell lines with endogenous wild-type p53 and in altered p53 cell lines with the replacement of wild-type p53 by a recombinant adenovirus (rAd-p53). Calpain inhibitor 1 treatment resulted in increased levels of activated p53, increased p21 protein, and activation of caspases. Cell lines with wild-type, but not mutated or null, p53 status arrested in G0/G1 and were sensitive to calpain inhibitor-induced apoptosis. Regardless of endogenous p53 status, calpain inhibitor treatment combined with rAd-p53, but not empty vector virus, enhanced apoptosis in tumor cell lines. These results demonstrate p53-dependent apoptosis induced by a calpain inhibitor and further suggest a role for calpains in the regulation of p53 activity and induction of apoptotic pathways.




This article has been cited by other articles:


Home page
FASEB J.Home page
D. G. Sedding, M. Homann, U. Seay, H. Tillmanns, K. T. Preissner, and R. C. Braun-Dullaeus
Calpain counteracts mechanosensitive apoptosis of vascular smooth muscle cells in vitro and in vivo
FASEB J, February 1, 2008; 22(2): 579 - 589.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
A. Jetzt, J. A. Howe, M. T. Horn, E. Maxwell, Z. Yin, D. Johnson, and C. C. Kumar
Adenoviral-Mediated Expression of a Kinase-Dead Mutant of Akt Induces Apoptosis Selectively in Tumor Cells and Suppresses Tumor Growth in Mice
Cancer Res., October 15, 2003; 63(20): 6697 - 6706.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. Sedarous, E. Keramaris, M. O'Hare, E. Melloni, R. S. Slack, J. S. Elce, P. A. Greer, and D. S. Park
Calpains Mediate p53 Activation and Neuronal Death Evoked by DNA Damage
J. Biol. Chem., July 3, 2003; 278(28): 26031 - 26038.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
R. W. Neumar, Y. A. Xu, H. Gada, R. P. Guttmann, and R. Siman
Cross-talk between Calpain and Caspase Proteolytic Systems During Neuronal Apoptosis
J. Biol. Chem., April 18, 2003; 278(16): 14162 - 14167.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
M. Ramachandra, I. Atencio, A. Rahman, M. Vaillancourt, A. Zou, J. Avanzini, K. Wills, R. Bookstein, and P. Shabram
Restoration of Transforming Growth Factor {beta} Signaling by Functional Expression of Smad4 Induces Anoikis
Cancer Res., November 1, 2002; 62(21): 6045 - 6051.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
N. O. Carragher, M. A. Westhoff, D. Riley, D. A. Potter, P. Dutt, J. S. Elce, P. A. Greer, and M. C. Frame
v-Src-Induced Modulation of the Calpain-Calpastatin Proteolytic System Regulates Transformation
Mol. Cell. Biol., January 1, 2002; 22(1): 257 - 269.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
R. Benetti, G. D. Sal, M. Monte, G. Paroni, C. Brancolini, and C. Schneider
The death substrate Gas2 binds m-calpain and increases susceptibility to p53-dependent apoptosis
EMBO J., June 1, 2001; 20(11): 2702 - 2714.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2000 by the American Association of Cancer Research.