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Cell Growth & Differentiation Vol. 11, 163-171, March 2000
© 2000 American Association for Cancer Research

Expression of the A-raf Proto-Oncogene in the Normal Adult and Embryonic Mouse1

Jennifer C. A. Luckett, Martin B. Hüser, Nikolaos Giagtzoglou2, Jane E. Brown and Catrin A. Pritchard3

Department of Biochemistry [J. C. A. L., M. B. H., N. G., C. A. P.] and Division of Biomedical Services [J. E. B.], University of Leicester, Leicester LE1 7RH, United Kingdom

We have determined the expression pattern of the A-raf proto-oncogene in the embryonic and adult mouse. Western blot analysis of protein lysates from tissues of adult mice show that p69A-raf is ubiquitously expressed, but that levels of expression vary among different tissues. To determine the cell-specific expression pattern of A-raf, we generated transgenic mice expressing the ß-galactosidase reporter gene from the A-raf promoter. We show that A-raf expression is highly specific within a given tissue, and we identify cell types expressing this gene in the adult testis, epididymis, vas deferens, seminal vesicle, ovary, oviduct, bladder, kidney, intestine, heart, spleen, thymus, and cerebellum. In the embryo, ubiquitous expression of the reporter gene is observed, but the highest levels of expression are specifically detected in the embryonic heart at stages 9.5–11.5 days post-coitum.




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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2000 by the American Association of Cancer Research.