CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feliciello, A.
Right arrow Articles by Gottesman, M. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feliciello, A.
Right arrow Articles by Gottesman, M. E.
Cell Growth & Differentiation Vol. 11, 649-654, December 2000
© 2000 American Association for Cancer Research


Articles

Thyroid Transcription Factor 1 Phosphorylation Is Not Required for Protein Kinase A-dependent Transcription of the Thyroglobulin Promoter 1

Antonio Feliciello2, Giovanna Allevato2, Anna Maria Musti, Daniele De Brasi, Adriana Gallo, Vittorio Enrico Avvedimento3 and Max E. Gottesman

Dipartimento di Biologia e Patologia Molecolare e Cellulare, Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche, Università "Federico II," 80131 Napoli, Italy [A. F., G. A., A. M. M., D. D. B., A. G., V. E. A.]; Dipartimento Farmacobiologico, Facoltà di Farmacia, Università della Calabria, 87100 Rende (Cosenza), Italy [A. M. M.]; Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro, 88100 Catanzaro, Italy [V. E. A.]; and Institute of Cancer Research, Columbia University, New York, New York 10032 [M. E. G.]

Abstract

Thyroid transcription factor 1 (TTF1) is a nuclear homeodomain protein that binds to and activates the promoters of several thyroid-specific genes, including that of the thyroglobulin gene (pTg). These genes are also positively regulated by thyroid-stimulating hormone/cyclic AMP (cAMP)/protein kinase A (PKA) signaling. We asked whether PKA directly activates TTF1. We show that cAMP/PKA activates pTg and a synthetic target promoter carrying TTF1 binding site repeats in several cell types. Activation depends on TTF1. Phosphopeptide mapping indicates that TTF1 is constitutively phosphorylated at multiple sites, and that cAMP stimulated phosphorylation of one site, serine 337, in vivo. However, alanine substitution at this residue or at all sites of phosphorylation did not reduce PKA activation of pTg. Thus, PKA stimulates TTF1 transcriptional activity in an indirect manner, perhaps by recruiting to or removing from the target promoter another regulatory factor(s).




This article has been cited by other articles:


Home page
J Biol ChemHome page
M. deFelice, D. Silberschmidt, R. DiLauro, Y. Xu, S. E. Wert, T. E. Weaver, C. J. Bachurski, J. C. Clark, and J. A. Whitsett
TTF-1 Phosphorylation Is Required for Peripheral Lung Morphogenesis, Perinatal Survival, and Tissue-specific Gene Expression
J. Biol. Chem., September 12, 2003; 278(37): 35574 - 35583.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. Billiard, S. S. Grewal, L. Lukaesko, P. J. S. Stork, and P. Rotwein
Hormonal Control of Insulin-like Growth Factor I Gene Transcription in Human Osteoblasts: DUAL ACTIONS OF cAMP-DEPENDENT PROTEIN KINASE ON CCAAT/ENHANCER-BINDING PROTEIN {delta}
J. Biol. Chem., August 17, 2001; 276(33): 31238 - 31246.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
J. Billiard, S. S. Grewal, L. Lukaesko, P. J. S. Stork, and P. Rotwein
Hormonal Control of Insulin-like Growth Factor I Gene Transcription in Human Osteoblasts: DUAL ACTIONS OF cAMP-DEPENDENT PROTEIN KINASE ON CCAAT/ENHANCER-BINDING PROTEIN {delta}
J. Biol. Chem., August 17, 2001; 276(33): 31238 - 31246.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 2000 by the American Association of Cancer Research.