CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kraemer, M.
Right arrow Articles by Binétruy, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kraemer, M.
Right arrow Articles by Binétruy, B.
Cell Growth & Differentiation Vol. 10, 193-200, March 1999
© 1999 American Association for Cancer Research

Rat Embryo Fibroblasts Transformed by c-Jun Display Highly Metastatic and Angiogenic Activities in Vivo and Deregulate Gene Expression of Both Angiogenic and Antiangiogenic Factors1

Michel Kraemer2, Roselyne Tournaire2, Valerie Dejong, Nicole Montreau, Dominique Briane, Claude Derbin and Bernard Binétruy3

Université Paris XIII, UFR Léonard de Vinci, Laboratoire d’Oncologie cellulaire et d’imagerie des tumeurs, 93017 Bobigny cedex [M.K., R.T., D.B., C.D.], and Institut de Recherche sur le Cancer, 94801, Villejuif, cedex [V.D., N.M., B.B.], France

The comparative tumorigenicity in rats and nude mice of cell lines derived from FR3T3 and transformed by either c-jun, ras, SV40 lt, or bovine papilloma virus type 1 (BPV1) oncogenes was investigated. c-Jun-transformed cells were as tumorigenic and metastatic as Ras-transformed cells. Latencies were short, and numerous pulmonary metastases were observed in all injected animals. In contrast, tumors induced by s.c. injection of SV40-transformed cells developed slower, and none of the animals who received injections i.v. presented with metastases. BPV1-transformed cells had an intermediate tumorigenic and metastatic activity. Microvessels present in the different tumors were revealed by immunostaining with Griffonia (Bandeiraea) Simplicifolia lectin 1. Tumors obtained with c-Jun-transformed cells exhibited more neovascularization than those induced by the other oncogenes. By comparison to FR3T3 cells or SV40- or BPV1-transformed cells, c-Jun-transformed fibroblasts repress the antiangiogenic thrombospondin-1 and SPARC genes, whereas we found that they express higher levels of gene expression of the angiogenic vascular endothelial growth factor. Finally, as compared with cells before passage in animals, thrombospondin-1, SPARC, and VEGF gene expression was also deregulated in cell lines isolated from primary tumors induced by BPV1-transformants. Our results indicate that the high transforming potential of c-Jun, evidenced as soon as transformation is established in vitro, correlates with deregulation of gene expression of both angiogenic and antiangiogenic factors leading to rapid neovascularization of tumors.




This article has been cited by other articles:


Home page
Cancer Res.Home page
U. E. Knies-Bamforth, S. B. Fox, R. Poulsom, G. I. Evan, and A. L. Harris
c-Myc Interacts with Hypoxia to Induce Angiogenesis In vivo by a Vascular Endothelial Growth Factor-Dependent Mechanism
Cancer Res., September 15, 2004; 64(18): 6563 - 6570.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
A. Alfranca, M. D. Gutierrez, A. Vara, J. Aragones, F. Vidal, and M. O. Landazuri
c-Jun and Hypoxia-Inducible Factor 1 Functionally Cooperate in Hypoxia-Induced Gene Transcription
Mol. Cell. Biol., January 1, 2002; 22(1): 12 - 22.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
R. Thomas, L. D. True, J. A. Bassuk, P. H. Lange, and R. L. Vessella
Differential Expression of Osteonectin/SPARC during Human Prostate Cancer Progression
Clin. Cancer Res., March 1, 2000; 6(3): 1140 - 1149.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1999 by the American Association of Cancer Research.