CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quinlan, M. P.
Right arrow Articles by Hyatt, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quinlan, M. P.
Right arrow Articles by Hyatt, J. L.
Cell Growth & Differentiation Vol. 10, 839-854, December 1999
© 1999 American Association for Cancer Research

Establishment of the Circumferential Actin Filament Network Is a Prerequisite for Localization of the Cadherin-Catenin Complex in Epithelial Cells1

Margaret P. Quinlan2 and Janice L. Hyatt

Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 38163

With the adhesion molecules, the actin cytoskeleton controls cell-cell and cell-substrate interactions and participates in transmembrane signaling. The relationships between actin and adhesion complexes at the sites of adhesion have been well documented. Here we investigate by a series of studies whether a relationship exists between actin organization and the localization and function of the components of the cadherin-catenin complex (CCC) that participates in the cell-cell adherens junction. Reversible actin depolymerization reversibly affects the peripheral distribution of CCCs. Mutations in adenovirus E1A and the small GTPase rac1, but not Ha-ras, disrupt the circumferential, cortical actin filament (CAF) network and the targeting of CCC components to the cell surface. Disruption of actin stress fibers or microtubules does not interfere with CCC localization and function. Constitutive loss of the apical cortical actin ring results in epithelial cells in which components of the CCCs are found only in intracellular vesicles and never at the surface. A kinetic analysis of the de novo appearance of the CAF network and the CCCs at the cell surface was also conducted. When F-actin was dissolved, surface CCC components were internalized. Reestablishment of CAFs required about 4 h, during which time E-cadherin and {alpha}-catenin were found first in a juxtanuclear location and then in intracellular vesicles or post-Golgi carriers, similar to what was observed in cells expressing mutant E1A or rac1. Thus, disruption of preexisting CCCs resulted in their internalization and recycling to the Golgi. It was only after the regeneration of the filamentous actin ring beneath the cell surface that peripheral localization of CCCs was observed. A similar result was observed with dominant negative rac1. These data suggest that the status of cortical actin is assessed and transduced and thereby regulates the transport and delivery of cadherin and catenins to the cell surface.




This article has been cited by other articles:


Home page
JCBHome page
W. Choi, B. R. Acharya, G. Peyret, M.-A. Fardin, R.-M. Mege, B. Ladoux, A. S. Yap, A. S. Fanning, and M. Peifer
Remodeling the zonula adherens in response to tension and the role of afadin in this response
J. Cell Biol., April 25, 2016; 213(2): 243 - 260.
[Abstract] [Full Text] [PDF]


Home page
J. Neurosci.Home page
J. C. Burns and J. T. Corwin
Responses to Cell Loss Become Restricted as the Supporting Cells in Mammalian Vestibular Organs Grow Thick Junctional Actin Bands That Develop High Stability
J. Neurosci., January 29, 2014; 34(5): 1998 - 2011.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
K. Noda, J. Zhang, S. Fukuhara, S. Kunimoto, M. Yoshimura, and N. Mochizuki
Vascular Endothelial-Cadherin Stabilizes at Cell-Cell Junctions by Anchoring to Circumferential Actin Bundles through {alpha}- and {beta}-Catenins in Cyclic AMP-Epac-Rap1 Signal-activated Endothelial Cells
Mol. Biol. Cell, February 15, 2010; 21(4): 584 - 596.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
J. K. Sawyer, N. J. Harris, K. C. Slep, U. Gaul, and M. Peifer
The Drosophila afadin homologue Canoe regulates linkage of the actin cytoskeleton to adherens junctions during apical constriction
J. Cell Biol., July 13, 2009; 186(1): 57 - 73.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
C. C. F. Homem and M. Peifer
Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis
Development, March 15, 2008; 135(6): 1005 - 1018.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
M. Elbert, D. Cohen, and A. Musch
PAR1b Promotes Cell-Cell Adhesion and Inhibits Dishevelled-mediated Transformation of Madin-Darby Canine Kidney Cells
Mol. Biol. Cell, August 1, 2006; 17(8): 3345 - 3355.
[Abstract] [Full Text] [PDF]


Home page
IOVSHome page
J. M. Gonzalez Jr, J. A. Faralli, J. M. Peters, J. R. Newman, and D. M. Peters
Effect of Heparin II Domain of Fibronectin on Actin Cytoskeleton and Adherens Junctions in Human Trabecular Meshwork Cells.
, July 1, 2006; 47(7): 2924 - 2931.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
T. Sato, N. Fujita, A. Yamada, T. Ooshio, R. Okamoto, K. Irie, and Y. Takai
Regulation of the Assembly and Adhesion Activity of E-cadherin by Nectin and Afadin for the Formation of Adherens Junctions in Madin-Darby Canine Kidney Cells
J. Biol. Chem., February 24, 2006; 281(8): 5288 - 5299.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
A. Yamada, K. Irie, T. Hirota, T. Ooshio, A. Fukuhara, and Y. Takai
Involvement of the Annexin II-S100A10 Complex in the Formation of E-cadherin-based Adherens Junctions in Madin-Darby Canine Kidney Cells
J. Biol. Chem., February 18, 2005; 280(7): 6016 - 6027.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
V. S. Conlin, S. B. Curtis, Y. Zhao, E. D. W. Moore, V. C. Smith, R. M. Meloche, B. B. Finlay, and A. M. J. Buchan
Helicobacter pylori Infection Targets Adherens Junction Regulatory Proteins and Results in Increased Rates of Migration in Human Gastric Epithelial Cells
Infect. Immun., September 1, 2004; 72(9): 5181 - 5192.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
D. Lallemand, M. Curto, I. Saotome, M. Giovannini, and A. I. McClatchey
NF2 deficiency promotes tumorigenesis and metastasis by destabilizing adherens junctions
Genes & Dev., May 1, 2003; 17(9): 1090 - 1100.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
S. K. Quadri, M. Bhattacharjee, K. Parthasarathi, T. Tanita, and J. Bhattacharya
Endothelial Barrier Strengthening by Activation of Focal Adhesion Kinase
J. Biol. Chem., April 11, 2003; 278(15): 13342 - 13349.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
E. Hellsten, D. J. Bernard, J. W. Owens, M. Eckhaus, S. F. Suchy, and R. L. Nussbaum
Sertoli Cell Vacuolization and Abnormal Germ Cell Adhesion in Mice Deficient in an Inositol Polyphosphate 5-Phosphatase
Biol Reprod, May 1, 2002; 66(5): 1522 - 1530.
[Abstract] [Full Text]


Home page
Am. J. Physiol. Cell Physiol.Home page
H. Lum and K. A. Roebuck
Oxidant stress and endothelial cell dysfunction
Am J Physiol Cell Physiol, April 1, 2001; 280(4): C719 - C741.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
J.J. Peluso, A. Pappalardo, and G. Fernandez
E-Cadherin-Mediated Cell Contact Prevents Apoptosis of Spontaneously Immortalized Granulosa Cells by Regulating Akt Kinase Activity
Biol Reprod, April 1, 2001; 64(4): 1183 - 1190.
[Abstract] [Full Text]


Home page
JCBHome page
E. E. Grevengoed, J. J. Loureiro, T. L. Jesse, and M. Peifer
Abelson kinase regulates epithelial morphogenesis in Drosophila
J. Cell Biol., December 24, 2001; 155(7): 1185 - 1198.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1999 by the American Association of Cancer Research.