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Cell Growth & Differentiation Vol. 10, 43-48, January 1999
© 1999 American Association for Cancer Research

Differential Regulation of Peroxisome Proliferator Activated Receptor {gamma}1 (PPAR{gamma}1) and PPAR{gamma}2 Messenger RNA Expression in the Early Stages of Adipogenesis1

Régis Saladin2,, 3, Lluis Fajas2, Sharon Dana, Yuan-Di Halvorsen, Johan Auwerx and Michael Briggs4

Ligand Pharmaceuticals, Inc., San Diego, California 92121-3016 [R. S., S. D., M. B.]; Institut National de la Santé et de la Recherche Médicale U325 and Département d’Athérosclérose, Institut Pasteur de Lille, 59019 Lille, France [L. F., J. A.]; and Zen-Bio, Inc., Research Triangle Park, North Carolina 27709 [Y-D. H.]

Adipocyte differentiation is driven by the expression and activation of three transcription factor families: the differentially expressed CAAT/enhancer binding proteins (C/EBPs) {alpha}, ß, and {delta}; the helix-loop-helix adipocyte differentiation and determination factor-1; and peroxisome proliferator activated receptor {gamma} (PPAR{gamma}), expressed as two isoforms, PPAR{gamma}1 and the adipocyte-specific PPAR{gamma}2. Overexpression of PPAR{gamma} can induce adipocyte differentiation; therefore, we analyzed the expression of the two PPAR{gamma} isoforms during early stages of differentiation to determine whether one was preferentially induced as an early determining event. Surprisingly, in the first 24 h, a 3–6-fold increase of PPAR{gamma}2 mRNA was observed, whereas PPAR{gamma}1 mRNA remained unchanged. PPAR{gamma}1 was induced 1 day later. Overexpression of C/EBPß has also been shown to induce adipocyte differentiation. A C/EBP site was identified only in the human PPAR{gamma}2 promoter. Its deletion blunted the response of PPAR{gamma}2 promoter to cotransfected C/EBPß or methylisobutylxanthine treatment. We hypothesize that PPAR{gamma}2 initiates adipocyte differentiation.




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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1999 by the American Association of Cancer Research.