CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ever, L.
Right arrow Articles by Don, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ever, L.
Right arrow Articles by Don, J.
Cell Growth & Differentiation Vol. 10, 19-26, January 1999
© 1999 American Association for Cancer Research

Two Alternatively Spliced Meig1 Messenger RNA Species Are Differentially Expressed in the Somatic and in the Germ-Cell Compartments of the Testis1

Leah Ever, Rachel Steiner, Sarah Shalom and Jeremy Don2

Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel

Previous studies regarding the transcriptional pattern of the murine Meig1 gene (formally designated meg1) suggested that its transcription is restricted to germ cells at the first meiotic prophase, in both primary spermatocytes and primary oocytes. However, protein analysis revealed that certain forms of the MEIG1 protein exist in testes of early postnatal pups at stages that have no germ cells in the testis, excluding very few primitive type A spermatogonia cells. This suggested that MEIG1 expression is not confined to germ cells. In this study, we show that testicular somatic cells do, indeed, express MEIG1. This is especially evident in Leydig cells, where this protein is highly abundant. We also demonstrate that alternatively spliced mRNAs of Meig1 are differentially transcribed in the germ cell and the somatic compartments of the testis. There is a very low level of somatic transcript, whether labile or transcriptionally regulated, in contrast to the abundant MEIG1 protein in the somatic cells. This implies that the somatic transcript is very efficiently translated and reconfirms that protein levels do not necessarily reflect transcript abundancy. Structural features of the Meig1 transcript that would be expected to inhibit translation are discussed in light of the efficient translation of this RNA species.




This article has been cited by other articles:


Home page
DevelopmentHome page
W. Li, W. Tang, M. E. Teves, Z. Zhang, L. Zhang, H. Li, K. J. Archer, D. L. Peterson, D. C. Williams Jr, J. F. Strauss III, et al.
A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella
Development, March 1, 2015; 142(5): 921 - 930.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
Z. Zhang, X. Shen, D. R. Gude, B. M. Wilkinson, M. J. Justice, C. J. Flickinger, J. C. Herr, E. M. Eddy, and J. F. Strauss III
MEIG1 is essential for spermiogenesis in mice
PNAS, October 6, 2009; 106(40): 17055 - 17060.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
A. Guais, B. Solhonne, N. Melaine, G. Guellaen, and F. Bulle
Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells
Biol Reprod, January 1, 2004; 70(1): 204 - 213.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1999 by the American Association of Cancer Research.