CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Azar, C. G.
Right arrow Articles by Brodeur, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Azar, C. G.
Right arrow Articles by Brodeur, G. M.

Cell Growth & Differentiation, Vol 1, Issue 9 421-428, Copyright © 1990 by American Association of Cancer Research


ARTICLES

Multiple defects of the nerve growth factor receptor in human neuroblastomas

CG Azar, NJ Scavarda, CP Reynolds and GM Brodeur
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.

Neuroblastoma is a tumor of postganglionic sympathetic origin, and nerve growth factor (NGF) is normally required for the survival and differentiation of sympathetic neuroblasts. Since the biological activity of NGF is mediated by the NGF receptor (NGFR), we hypothesized that defects in the NGF/NGFR pathway may play a role in maintenance of the undifferentiated state of neuroblastomas. To test this hypothesis, we examined the structure of the NGFR at the DNA, RNA, and protein levels in a panel of 10 neuroblastoma cell lines. In addition, we examined the function of the NGFR in these lines by analysis of NGF binding kinetics, as well as by the ability of NGF to induce c-fos expression and neurite outgrowth. Southern blot analysis showed that all 10 cell lines possess apparently normal NGFR genes. Northern blot and ligand binding/immunoprecipitation assays revealed four receptor-positive cell lines (NGP, NLF, SK-N-SH, and LA-N-6), with NGFR mRNA and protein of expected sizes (3.8 kilobases and Mr approximately 75,000, respectively). NGF binding assays and Scatchard analyses were performed on the four NGFR-positive lines. The NGP line possesses only low-affinity receptor (Kd approximately 3.5 x 10(-9)), whereas the other three lines express both low- and high-affinity forms (Kd approximately 10(-9) and Kd approximately 10(-11), respectively). However, none of the 10 lines exhibited a response to NGF treatment as assayed by c-fos mRNA induction and neurite extension.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
Ann Clin Lab SciHome page
K. Yamauchi, M. Tozuka, E. Hidaka, I. Ueno, K. Matsuda, and T. Katsuyama
Internalization of {beta}-Amyloid Causes Downregulation of Apolipoprotein E mRNA Expression in Neuroblastoma Cells
Ann. Clin. Lab. Sci., January 1, 2003; 33(1): 68 - 78.
[Abstract] [Full Text] [PDF]


Home page
Ann Clin Lab SciHome page
K. Yamauchi, M. Tozuka, H. Hidaka, T. Nakabayashi, M. Sugano, and T. Katsuyama
Isoform-specific Effect of Apolipoprotein E on Endocytosis of {beta}-Amyloid in Cultures of Neuroblastoma Cells
Ann. Clin. Lab. Sci., January 1, 2002; 32(1): 65 - 74.
[Abstract] [Full Text] [PDF]


Home page
Cell Growth Differ.Home page
A. Edsjo, B. Hallberg, S. Fagerstrom, C. Larsson, H. Axelson, and S. Pahlman
Differences in Early and Late Responses between Neurotrophin-stimulated trkA- and trkC-transfected SH-SY5Y Neuroblastoma Cells
Cell Growth Differ., January 1, 2001; 12(1): 39 - 50.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
B. B. Chang, S. P. Persengiev, J. G. de Diego, M. P. Sacristan, D. M. Zanca, and D. L. Kilpatrick
Proximal Promoter Sequences Mediate Cell-specific and Elevated Expression of the Favorable Prognosis Marker TrkA in Human Neuroblastoma Cells
J. Biol. Chem., January 2, 1998; 273(1): 39 - 44.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1990 by the American Association of Cancer Research.