CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guo, X. J.
Right arrow Articles by Denhardt, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guo, X. J.
Right arrow Articles by Denhardt, D. T.

Cell Growth & Differentiation, Vol 1, Issue 7 333-338, Copyright © 1990 by American Association of Cancer Research


ARTICLES

Identification of a serum-inducible messenger RNA (5B10) as the mouse homologue of calcyclin: tissue distribution and expression in metastatic, ras-transformed NIH 3T3 cells

XJ Guo, AF Chambers, CL Parfett, P Waterhouse, LC Murphy, RE Reid, AM Craig, DR Edwards and DT Denhardt
Cancer Research Laboratory, University of Western Ontario, London, Canada.

A mouse mRNA, provisionally designated 5B10, has been cloned based on its inducibility by serum in quiescent murine fibroblasts. Here we report the full-length complementary DNA sequence and a partial characterization. There are about five copies of the gene in the mouse genome. Sequence analysis of the 5B10 coding region reveals 94 and 97% amino acid identity to human and rat calcyclin, respectively. Although the coding region has been highly conserved during evolution of the rodent and human genomes, the untranslated flanking sequences differ significantly. A protein of Mr about 8000 was produced by in vitro translation of the mRNA transcribed in vitro from 5B10 complementary DNA in a riboprobe vector. An antiserum raised against a portion of the predicted human calcyclin protein cross-reacted with this mouse protein. 5B10 mRNA was found in greatest amount in organs containing proliferating cells, e.g., epidermis, skin, stomach, uterus of pregnant mouse, placenta, and decidua. Brain, liver, mature thymus, and skeletal muscle had little or no detectable 5B10 mRNA. 5B10 mRNA levels were higher in cells treated with 7,12-dimethylbenzanthracene and 12-O-tetradecanoylphorbol-13-acetate than in their normal counterparts, suggesting a role in tumorigenesis. In addition, high 5B10 mRNA levels were associated with metastatic ability in a series of ras-transformed cells, in proportion to levels of ras p21 expressed by the cells, implicating 5B10 even more deeply in carcinogenesis.


This article has been cited by other articles:


Home page
J Biol ChemHome page
M. Nowotny, S. Bhattacharya, A. Filipek, A. M. Krezel, W. Chazin, and J. Kuznicki
Characterization of the Interaction of Calcyclin (S100A6) and Calcyclin-binding Protein
J. Biol. Chem., October 6, 2000; 275(40): 31178 - 31182.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
K. Komatsu, A. Andoh, S. Ishiguro, N. Suzuki, H. Hunai, Y. Kobune-Fujiwara, M. Kameyama, J. Miyoshi, H. Akedo, and H. Nakamura
Increased Expression of S100A6 (Calcyclin), a Calcium-binding Protein of the S100 Family, in Human Colorectal Adenocarcinomas
Clin. Cancer Res., January 1, 2000; 6(1): 172 - 177.
[Abstract] [Full Text]


Home page
J Biol ChemHome page
T. B. Stradal and M. Gimona
Ca2+-dependent Association of S100A6 (Calcyclin) with the Plasma Membrane and the Nuclear Envelope
J. Biol. Chem., October 29, 1999; 274(44): 31593 - 31596.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. Nowotny, S. Bhattacharya, A. Filipek, A. M. Krezel, W. Chazin, and J. Kuznicki
Characterization of the Interaction of Calcyclin (S100A6) and Calcyclin-binding Protein
J. Biol. Chem., October 6, 2000; 275(40): 31178 - 31182.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1990 by the American Association of Cancer Research.