CG&D
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nose, K.
Right arrow Articles by Kuroki, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nose, K.
Right arrow Articles by Kuroki, T.

Cell Growth & Differentiation, Vol 1, Issue 11 511-518, Copyright © 1990 by American Association of Cancer Research


ARTICLES

Isolation of a gene sequence induced later by tumor-promoting 12-O-tetradecanoylphorbol-13-acetate in mouse osteoblastic cells (MC3T3-E1) and expressed constitutively in ras-transformed cells

K Nose, H Saito and T Kuroki
Department of Cancer Cell Research, University of Tokyo, Japan.

Growth factors and tumor-promoting phorbol esters induce various types of "early response" genes, most of which encode transcription factors. To identify possible target genes for these early response gene products, we isolated complementary DNA clones of genes that were induced later by 12-O-tetradecanoylphorbol-13-acetate. A complementary DNA library was constructed using mRNA from mouse osteoblastic cells (MC3T3-E1) treated with 12-O-tetradecanoylphorbol-13-acetate for 4 h, and the library was screened by differential hybridization. Five independent clones were isolated. All of these genes were induced maximally 4 to 8 h after 12-O-tetradecanoylphorbol-13-acetate addition. Sequencing showed that three of them (OTS-1, -3, and -4) were those of the metallothionein II gene, and one (OTS-5) was identical to the osteopontin/2ar gene. The other (OTS-8) had no significant homology with known sequences and was considered to be a novel gene. The induction of OTS-5 and OTS-8 was decreased in the presence of cycloheximide. In mice, OTS-8 mRNA was detected in the lungs but not at appreciable levels in other organs. Open reading frame of OTS-8 complementary DNA and its in vitro transcription/translation product suggested that this gene encodes an Mr 26,000 protein. OTS-8 mRNA was induced by epidermal growth factor but not by serum. The steady-state levels of OTS-5 and OTS-8 mRNAs were significantly elevated in v-ras-transformed MC3T3 cells.


This article has been cited by other articles:


Home page
J Biol ChemHome page
H. Krishnan, J. A. Ochoa-Alvarez, Y. Shen, E. Nevel, M. Lakshminarayanan, M. C. Williams, M. I. Ramirez, W. T. Miller, and G. S. Goldberg
Serines in the Intracellular Tail of Podoplanin (PDPN) Regulate Cell Motility
J. Biol. Chem., April 26, 2013; 288(17): 12215 - 12221.
[Abstract] [Full Text] [PDF]


Home page
Cold Spring Harb Perspect MedHome page
I. Choi, S. Lee, and Y.-K. Hong
The New Era of the Lymphatic System: No Longer Secondary to the Blood Vascular System
Cold Spring Harb Perspect Med, April 1, 2012; 2(4): a006445 - a006445.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Navarro, R. E. Perez, M. Rezaiekhaligh, S. M. Mabry, and I. I. Ekekezie
T1{alpha}/podoplanin is essential for capillary morphogenesis in lymphatic endothelial cells
Am J Physiol Lung Cell Mol Physiol, October 1, 2008; 295(4): L543 - L551.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
K. Zhang, C. Barragan-Adjemian, L. Ye, S. Kotha, M. Dallas, Y. Lu, S. Zhao, M. Harris, S. E. Harris, J. Q. Feng, et al.
E11/gp38 Selective Expression in Osteocytes: Regulation by Mechanical Strain and Role in Dendrite Elongation
Mol. Cell. Biol., June 15, 2006; 26(12): 4539 - 4552.
[Abstract] [Full Text] [PDF]


Home page
Eur Respir JHome page
M.C. McElroy and M. Kasper
The use of alveolar epithelial type I cell-selective markers to investigate lung injury and repair
Eur. Respir. J., October 1, 2004; 24(4): 664 - 673.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
Y. Kato, N. Fujita, A. Kunita, S. Sato, M. Kaneko, M. Osawa, and T. Tsuruo
Molecular Identification of Aggrus/T1{alpha} as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors
J. Biol. Chem., December 19, 2003; 278(51): 51599 - 51605.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
G. Zhang, B. He, and G. F. Weber
Growth Factor Signaling Induces Metastasis Genes in Transformed Cells: Molecular Connection between Akt Kinase and Osteopontin in Breast Cancer
Mol. Cell. Biol., September 15, 2003; 23(18): 6507 - 6519.
[Abstract] [Full Text] [PDF]


Home page
EMBO J.Home page
V. Schacht, M. I. Ramirez, Y.-K. Hong, S. Hirakawa, D. Feng, N. Harvey, M. Williams, A. M. Dvorak, H. F. Dvorak, G. Oliver, et al.
T1{alpha}/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema
EMBO J., July 15, 2003; 22(14): 3546 - 3556.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
F. G. Scholl, C. Gamallo, S. Vilar?o, and M. Quintanilla
Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes
J. Cell Sci., December 15, 1999; 112(24): 4601 - 4613.
[Abstract] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. A. Gutierrez, R. F. Gonzalez, and L. G. Dobbs
Mechanical distension modulates pulmonary alveolar epithelial phenotypic expression in vitro
Am J Physiol Lung Cell Mol Physiol, February 1, 1998; 274(2): L196 - L202.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. C. McElroy, J.-F. Pittet, L. Allen, J. P. Wiener-Kronish, and L. G. Dobbs
Biochemical detection of type I cell damage after nitrogen dioxide-induced lung injury in rats
Am J Physiol Lung Cell Mol Physiol, December 1, 1997; 273(6): L1228 - L1234.
[Abstract] [Full Text] [PDF]


Home page
J Biol ChemHome page
M. I. Ramirez, A. K. Rishi, Y. X. Cao, and M. C. Williams
TGT3, Thyroid Transcription Factor I, and Sp1 Elements Regulate Transcriptional Activity of the 1.3-Kilobase Pair Promoter ofT1{alpha}, a Lung Alveolar Type I Cell Gene
J. Biol. Chem., October 17, 1997; 272(42): 26285 - 26294.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cell Growth & Differentiation
Copyright © 1990 by the American Association of Cancer Research.